<i>Acinetobacter septicus</i>
            sp. nov. Association with a Nosocomial Outbreak of Bacteremia in a Neonatal Intensive Care Unit

Kılıç, Abdullah; Li, Haijing; Mellmann, Alexander; Başustaoğlu, Ahmet; Kul, Mustafa; Şenses, Zeynep; Aydoğan, Hakan; Stratton, Charles W.; Harmsen, Dag; Tang, Yi‐Wei

doi:10.1128/jcm.01876-07

Cited by 49 publications

(49 citation statements)

References 42 publications

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“…3). Additionally, all A. ursingii strains tested (BAA-617 T , NIPH 177, NIPH 375, NIPH 706;Nemec et al, 2000Nemec et al, , 2001, AK001 (Kilic et al, 2008;Nemec et al, 2001Nemec et al, , 2008 and A. beijerinckii also consistently displayed UV-induced mutagenesis. The mutations were only observed after overnight growth, as no rifampicin-resistant mutants were present immediately after UV-C exposure (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Diverse responses to UV light exposure in Acinetobacter include the capacity for DNA damage-induced mutagenesis in the opportunistic pathogens Acinetobacter baumannii and Acinetobacter ursingii

et al. 2012

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Error-prone and error-free DNA damage repair responses that are induced in most bacteria after exposure to various chemicals, antibiotics or radiation sources were surveyed across the genus Acinetobacter. The error-prone SOS mutagenesis response occurs when DNA damage induces a cell's umuDC-or dinP-encoded error-prone polymerases. The model strain Acinetobacter baylyi ADP1 possesses an unusual, regulatory umuD allele (umuDAb) with an extended 59 region and only incomplete fragments of umuC. Diverse Acinetobacter species were investigated for the presence of umuDC and their ability to conduct UV-induced mutagenesis. Unlike ADP1, most Acinetobacter strains possessed multiple umuDC loci containing either umuDAb or a umuD allele resembling that of Escherichia coli. The nearly omnipresent umuDAb allele was the ancestral umuD in Acinetobacter, with horizontal gene transfer accounting for over half of the umuDC operons. Despite multiple umuD(Ab)C operons in many strains, only three species conducted UV-induced mutagenesis: Acinetobacter baumannii, Acinetobacter ursingii and Acinetobacter beijerinckii. The type of umuDC locus or mutagenesis phenotype a strain possessed was not correlated with its error-free response of survival after UV exposure, but similar diversity was apparent. The survival of 30 Acinetobacter strains after UV treatment ranged over five orders of magnitude, with the Acinetobacter calcoaceticus-A. baumannii (Acb) complex and haemolytic strains having lower survival than non-Acb or non-haemolytic strains. These observations demonstrate that a genus can possess a range of DNA damage response mechanisms, and suggest that DNA damage-induced mutation could be an important part of the evolution of the emerging pathogens A. baumannii and A. ursingii. INTRODUCTIONWhen bacteria are exposed to DNA-damaging agents, such as UV light or chemical mutagens, a wide variety of SOS response genes (genes that play critical roles in repairing damaged DNA) are specifically induced (Friedberg et al., 1995;Walker, 1996). In the model developed by experimentation in Escherichia coli, SOS genes are negatively regulated by the LexA repressor binding to the SOS box in the promoter (Mount et al., 1972). DNA damage causes the activation of RecA, which facilitates LexA self-cleavage and releases the repression of SOS genes such as umuDC (Little et al., 1980;1981). UmuD initially causes a pause in cell division while DNA repair and replication occurs (Opperman et al., 1999). However, within minutes, UmuD homodimerizes and carries out intermolecular self-cleavage, facilitated by activated RecA* interaction (Nohmi et al., 1988). Two cleaved UmuD9 molecules then associate with UmuC to form DNA polymerase V, which carries out error-prone, trans-lesion DNA replication (SOS mutagenesis) (Reuven et al., 1999;Tang et al., 1999). Another errorprone polymerase commonly involved in SOS mutagenesis as part of the DNA damage response is DinP (also called DinB), DNA polymerase IV, which can function either by itself (Kim et al., 1997) or with U...

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Section: Resultsmentioning

confidence: 99%

Diverse responses to UV light exposure in Acinetobacter include the capacity for DNA damage-induced mutagenesis in the opportunistic pathogens Acinetobacter baumannii and Acinetobacter ursingii

et al. 2012

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“…Of those, 60 isolates (0.4‰; see Table S1 in the supplemental material) had a 16S rRNA gene homology of Ͻ99% to members of accepted taxa on the date of the first interpretation. A total of 11 of the 60 sequences with a 16S rRNA homology of Ͻ99% in the first-time analysis could be allocated to a species established during the study term as a novel species by others: Acinetobacter septicus (16,20), Brevibacterium ravenspurgense (17), Corynebacterium freiburgense (12), Corynebacterium massiliense (n ϭ 2) (18), C. mastitidis (10,18), C. pyruviciproducens (26), C. ureicelerivorans (11,31), Neisseria zoodegmatis (28), Paenibacillus barengoltzii (21), and the reclassified Campy- lobacter ureolyticus (previously known as Bacteroides ureolyticus) (27). We calculated dendrograms to assess phylogenetic relationships (Fig.…”

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confidence: 99%

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confidence: 99%

Recognition of Potentially Novel Human Disease-Associated Pathogens by Implementation of Systematic 16S rRNA Gene Sequencing in the Diagnostic Laboratory

Keller

Rampini²,

Büchler

et al. 2010

J Clin Microbiol

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Clinical isolates that are difficult to identify by conventional means form a valuable source of novel human pathogens. We report on a 5-year study based on systematic 16S rRNA gene sequence analysis. We found 60 previously unknown 16S rRNA sequences corresponding to potentially novel bacterial taxa. For 30 of 60 isolates, clinical relevance was evaluated; 18 of the 30 isolates analyzed were considered to be associated with human disease.16S rRNA gene sequence analysis is broadly considered the "gold standard" in bacterial identification (6,29). In daily clinical diagnostics, accurate bacterial identification is essential in judging whether a bacterial isolate is to be considered the causative agent of an infectious disease or merely a colonizer. In our study, we aimed to characterize the bacterial diversity encountered in a diagnostic laboratory by revealing potentially novel, clinically relevant species, according to the current species definition by the Clinical and Laboratory Standards Institute (22).Routine 16S rRNA gene sequencing is implemented in our laboratory and is a fixed part of our diagnostic algorithms for identification of bacterial isolates (1, 2, 32). We retrospectively reanalyzed 16S rRNA gene sequences collected during 2004 to 2008 to identify potentially novel bacterial taxa of clinical relevance. The Institute of Medical Microbiology (IMM) serves the 850-bed University Hospital of Zurich and surrounding smaller hospitals. Bacterial isolates from blood, cerebrospinal fluid, wounds, joint aspirates, respiratory samples, genitourinary swabs, feces, and urine were recovered by culture on appropriate media according to standard procedures (19). Isolates that could not be identified by phenotypic methods underwent sequencing. 16S rRNA gene analysis was performed as previously described (1). Homology analyses were performed using the SmartGene Integrated Database Network System (IDNS) (24) and NCBI GenBank databases. For the first screening of our large data collection, we selected isolates with sequence homology of Ͻ99.0% to members of described taxa, regarding these as potentially novel species; isolates with sequence homology of Ͻ95% were regarded as representatives of a novel genus (2). The boundary for novel families was Ͻ87.5% homology and, for novel orders, Ͻ78.4% 16S rRNA sequence homology (30). After the first screening, we used more stringent cutoff values (Ͻ97.5% for species) for taxa with significant interspecies 16S rRNA divergence; i.e., members of the Paenibacillaceae family and the Clostridiales order (6, 25).During the 5-year study period, 1,663 cultured isolates were subjected to 16S rRNA gene sequence analysis (Table 1). Of those, 60 isolates (0.4‰; see Table S1 in the supplemental material) had a 16S rRNA gene homology of Ͻ99% to members of accepted taxa on the date of the first interpretation. A total of 11 of the 60 sequences with a 16S rRNA homology of Ͻ99% in the first-time analysis could be allocated to a species established during the study term as a novel species by othe...

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“…в последние десятилетия Acinetobacter baumannii стал клинически значи-мым патогеном, вызывающим широкий спектр ИСМП, а также инфекций, возникающих в зо-нах военных действий и стихийных бедствий [9]. Однако ряд исследований показали, что необыч-ные и недавно описанные виды Acinetobacter, такие как Acinetobacter septicus и Acinetobacter bereziniae также вовлечены в эпидемический процесс, а некоторые из них были устойчивы к карбапенемам [10][11][12].…”

Section: Introductionunclassified

Prevalence Acinetobacter spp. in Kemerovo Region Healthcare Settings

Шмакова¹,

Штернис²,

Желнина³

et al. 2018

Epidemiology and Vaccine Prevention

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ВведениеЭпидемиология инфекций, связанных с оказа-нием медицинской помощи (ИСМП), вызванных возбудителями группы сапронозов, представляет для медицинского сообщества как научный, так и практический интерес. Он обусловлен:

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Acinetobacter septicus sp. nov. Association with a Nosocomial Outbreak of Bacteremia in a Neonatal Intensive Care Unit

Cited by 49 publications

References 42 publications

Diverse responses to UV light exposure in Acinetobacter include the capacity for DNA damage-induced mutagenesis in the opportunistic pathogens Acinetobacter baumannii and Acinetobacter ursingii

Diverse responses to UV light exposure in Acinetobacter include the capacity for DNA damage-induced mutagenesis in the opportunistic pathogens Acinetobacter baumannii and Acinetobacter ursingii

Recognition of Potentially Novel Human Disease-Associated Pathogens by Implementation of Systematic 16S rRNA Gene Sequencing in the Diagnostic Laboratory

Prevalence Acinetobacter spp. in Kemerovo Region Healthcare Settings

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