<i>Aeromonas hydrophila</i>: Ecology and Toxigenicity of Isolates from an Estuary

Kaper, James B.; Lockman, Hank; Colwell, Rita R.; Joseph, Sam W.

doi:10.1111/j.1365-2672.1981.tb00900.x

Cited by 207 publications

(127 citation statements)

References 58 publications

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“…(1980) antimicrobial pressure. While we were able to achieve transfer Boonyaratpalin, S. (1989) to receive large numbers of bacteria (Austin 1983) Kaper et al 1981). Restriction of the use of drugs Aeromonas and Plesiomonas.…”

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confidence: 97%

Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from cultured fish, Telapia ( Telapia mossambica )

Radu¹,

Rusul²,

Mutalib³

et al. 1997

Letters in Applied Microbiology

104

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Strains of Aeromonas hydrophila isolates from skin lesions of the common freshwater fish, Telapia mossambica, were screened for the presence of plasmid DNA by agarose gel electrophoresis and tested for susceptibility to 10 antimicrobial agents. Of the 21 fish isolates examined, all were resistant to ampicillin and sensitive to gentamycin. Most isolates were resistant to streptomycin (57%), tetracycline (48%) and erythromycin (43%). While seven of 21 isolates harboured plasmids, with sizes ranging from 3 to 63·4 kilobase pair (kb), it was only possible to associate the presence of a plasmid with antibiotic resistance (ampicillin and tetracycline) in strain AH11. Both the plasmid and the associated antimicrobial resistance could be transferred to an Escherichia coli recipient by single‐step conjugation at a frequency of 4·3×10−3 transconjugants per donor cell.

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confidence: 97%

Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from cultured fish, Telapia ( Telapia mossambica )

Radu¹,

Rusul²,

Mutalib³

et al. 1997

Letters in Applied Microbiology

104

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“…Therefore, the presence of these bacteria, by itself, is not indicative of disease and, consequently, stress is often considered to be a contributing factor in outbreaks of disease caused by these bacteria. (16,17,19,26).…”

Section: Discussionmentioning

confidence: 99%

Pathological findings of experimental Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)

Yardımcı¹,

Aydin²

2011

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:In this study, clinical findings with macroscopic and microscopic pathologic changes of the tissues and organs after necropsy examination at the 1 st , 2 nd , 3 rd , 5 th and 7 th days following intraperitoneally injection of Nile tilapia (Oreochromis niloticus) with Aeromonas hydrophila was aimed to be observed. Clinically; weakness, anorexia, swimming closer to surface, darkness in color with hyperemia and lysis of the fins were observed. In the macroscopic examination the liver was seen to be yellowish brown and crispy with haemorrhagic and greyish white foci on the surface. The gall bladder was tightly full with emerald green bile. The kidney and hearth had haemorrhagic foci whilst intestine lumina observed to be filled with yellow coloured mucoid liquid. The degenerative changes, cytoplasmic fat vacuols and lymphocyte infiltration in lever with focal necrosis of hepatocytes and pancreatic cells were observed histologically. The skin, spleen, gill and eyes did not reveal any significant symptoms. The haemorrhagies and intensive lymphocyte infiltrations in liver, kidney and hearth both in macroscopic and microscopic levels revealed a visceral haemorrhagic septicemia. In this sense; clinical, macroscopic and microscopic findings observed during the present study complied with the Aeromonas hydrophila infections recorded in the other fresh water fishes.

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“…Aerolysin is a pore-forming toxin secreted by the human pathogen Aeromonas hydrophila and has been shown to be an important virulence factor produced by this bacterium (1)(2)(3)(4)(5). A. hydrophila has been implicated in a variety of diseases ranging from gastroenteritis to deep wound infection and septicemia.…”

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confidence: 99%

Aerolysin Induces G-protein Activation and Ca2+Release from Intracellular Stores in Human Granulocytes

Krause¹,

Fivaz²,

Monod³

et al. 1998

Journal of Biological Chemistry

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Aerolysin is a pore-forming toxin that plays a key role in the pathogenesis of Aeromonas hydrophila infections. In this study, we have analyzed the effect of aerolysin on human granulocytes (HL-60 cells). Proaerolysin could bind to these cells, was processed into active aerolysin, and led to membrane depolarization, indicating that granulocytes are potential targets for this toxin. Fura-2 measurements were used to analyze the effect of aerolysin on cytosolic [Ca 2؉] homeostasis. As expected for a pore-forming toxin, aerolysin addition led to Ca 2؉ influx across the plasma membrane. In addition, the toxin triggered Ca 2؉ release from agonist and thapsigargin-sensitive intracellular Ca 2؉ stores. This Ca 2؉ release was independent of the aerolysin-induced Ca 2؉ influx and occurred in two kinetically distinct phases: an initial rapid and transient phase and a second, more sustained, phase. The first, but not the second phase was sensitive to pertussis toxin. Activation of pertussis toxin-sensitive G-proteins appeared to be a consequence of pore formation, rather than receptor activation through aerolysinbinding, as it: (i) was not observed with a binding competent, insertion-incompetent aerolysin mutant, (ii) had a marked lag time, and (iii) was also observed in response to other bacterial pore-forming toxins (staphylococcal ␣-toxin, streptolysin O) which are thought to bind to different receptors. G-protein activation through pore-forming toxins stimulated cellular functions, as evidenced by pertussis toxin-sensitive chemotaxis. Our results demonstrate that granulocytes are potential target cells for aerolysin and that in these cells, Ca 2؉ signaling in response to a pore-forming toxin involves G-proteindependent cell activation and Ca 2؉ release from intracellular stores.

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Aeromonas hydrophila: Ecology and Toxigenicity of Isolates from an Estuary

Cited by 207 publications

References 58 publications

Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from cultured fish, Telapia ( Telapia mossambica )

Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from cultured fish, Telapia ( Telapia mossambica )

Pathological findings of experimental Aeromonas hydrophila infection in Nile tilapia (Oreochromis niloticus)

Aerolysin Induces G-protein Activation and Ca2+Release from Intracellular Stores in Human Granulocytes

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