<i>Aeromonas hydrophila</i>
            with Plasmid-Borne Class A Extended-Spectrum β-Lactamase TEM-24 and Three Chromosomal Class B, C, and D β-Lactamases, Isolated from a Patient with Necrotizing Fasciitis

Fosse, T.; Giraud‐Morin, C.; Madinier, I.; Mantoux, F.; Lacour, Jean‐Philippe; Ortonne, Jean‐Paul

doi:10.1128/aac.48.6.2342-2343.2004

Cited by 36 publications

(26 citation statements)

References 13 publications

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“…Much less frequently, sporadic cases of infection involving aeromonads have been published where the infecting strain possessed a class A ␤-lactamase belonging to the TEM family of ESBLs, a trait typically associated with the family Enterobacteriaceae. Most of these ceftazidime-resistant infections have been reported from France, where an outbreak clone of Enterobacter aerogenes possessing TEM-24 may have horizontally transferred this 180-kb plasmid to different Aeromonas species (95,191). Aeromonas isolates containing class B MBLs are extremely problematic, as they cannot routinely be detected using commercial products such as Etest for ESBLs (AB Biodisk, Solna, Sweden).…”

Section: ␤-Lactamases and Extended-spectrum ␤-Lactamases (Esbls)mentioning

confidence: 99%

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

2010

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SUMMARY Over the past decade, the genus Aeromonas has undergone a number of significant changes of practical importance to clinical microbiologists and scientists alike. In parallel with the molecular revolution in microbiology, several new species have been identified on a phylogenetic basis, and the genome of the type species, A. hydrophila ATCC 7966, has been sequenced. In addition to established disease associations, Aeromonas has been shown to be a significant cause of infections associated with natural disasters (hurricanes, tsunamis, and earthquakes) and has been linked to emerging or new illnesses, including near-drowning events, prostatitis, and hemolytic-uremic syndrome. Despite these achievements, issues still remain regarding the role that Aeromonas plays in bacterial gastroenteritis, the extent to which species identification should be attempted in the clinical laboratory, and laboratory reporting of test results from contaminated body sites containing aeromonads. This article provides an extensive review of these topics, in addition to others, such as taxonomic issues, microbial pathogenicity, and antimicrobial resistance markers.

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Section: ␤-Lactamases and Extended-spectrum ␤-Lactamases (Esbls)mentioning

confidence: 99%

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

2010

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“…(33, 58, 102, 103). In one particularly disturbing report, a plasmid-borne extended-spectrum ␤-lactamase and three chromosomal ␤-lactamases were isolated from a patient with necrotizing fasciitis (27). AHA2368 and -3370 encode putative phenazine (antibiotic) biosynthesis proteins, and products of AHA2416, -2375, and -1645 contain domains found in antibiotic biosynthesis monooxygenases.…”

Section: Vol 188 2006mentioning

confidence: 99%

Genome Sequence of Aeromonas hydrophila ATCC 7966 ^T : Jack of All Trades

et al. 2006

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“…(10, 34) and actually bear an increasing clinical importance. From a functional standpoint, class C ␤-lactamases are typically characterized by a very efficient hydrolysis of narrow-spectrum cephalosporins but are also able to degrade more recently found extended-spectrum cephalosporins and cephamycins and, thus, can confer resistance to these agents, especially in strains where the ␤-lactamase is derepressed or plasmid encoded (9,11,34).Aeromonads are ubiquitous waterborne organisms, and some Aeromonas species are known to cause human gastroenteritis or serious opportunistic infections, such as septicemia and cellulitis (20,27). Since these species are present in both the environment and the clinical setting, aeromonads have been identified as a reservoir of antibiotic resistance genes, including ␤-lactamase determinants such as the bla FOX -like and bla CMY-1 -like genes (19,24,34), which have been successfully transferred to more important opportunistic human pathogens, in particular members of the Enterobacteriaceae.…”

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confidence: 99%

Genetic and Biochemical Characterization of TRU-1, the Endogenous Class C β-Lactamase from Aeromonas enteropelogenes

Luca

Giraud‐Morin

Rossolini

et al. 2010

Antimicrob Agents Chemother

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Aeromonas enteropelogenes (formerly A. tructi) was described to be an ampicillin-susceptible and cephalothinresistant Aeromonas species, which suggests the production of a cephalosporinase. Strain ATCC 49803 was susceptible to amoxicillin, cefotaxime, and imipenem but resistant to cefazolin (MICs of 2, 0.032, 0.125, and >256 g/ml, respectively) and produced an inducible ␤-lactamase. Cefotaxime-resistant mutants (MIC, 32 g/ml) that showed constitutive ␤-lactamase production could be selected in vitro. The gene coding for the cephalosporinase of A. enteropelogenes ATCC 49803 was cloned, and its biochemical properties were investigated. Escherichia coli transformants showing resistance to various ␤-lactams carried a 3.5-kb plasmid insert whose sequence revealed a 1,146-bp open reading frame (ORF) encoding a class C ␤-lactamase, named TRU-1, showing the highest identity scores with A. punctata CAV-1 (75%), A. salmonicida AmpC (75%), and A. hydrophila CepH (71%). The bla TRU-1 locus includes open reading frames (ORFs) showing significant homology with genes found in the genomes of other Aeromonas species, although it exhibits a different organization, as reflected by the presence of additional ORFs located downstream of the ␤-lactamase gene in the A. hydrophila and A. salmonicida genomes. Specific PCR assays were negative for cphA-like and bla OXA-12 -like genes in three A. enteropelogenes ATCC strains. Purified TRU-1 showed a broad substrate profile, efficiently hydrolyzing benzylpenicillin, cephalothin, cefoxitin, and, although with significantly lower turnover rates, oxyiminocephalosporins. Cephaloridine and cefepime were poorly recognized by the enzyme, as reflected by the high K m values observed with these substrates. Thus far, A. enteropelogenes represents the only known example of an Aeromonas species that produces only one ␤-lactamase belonging to molecular class C.Resistance to ␤-lactam antibiotics in Gram-negative bacilli is mediated mainly by the production of ␤-lactamases, which are divided into four major molecular classes, classes A, B, C, and D (3, 11). Genes encoding AmpC (class C) ␤-lactamases are generally found on the chromosome of many Gram-negative, aerobic, rod-shaped bacteria and are usually inducible (38). About 20 years ago, plasmid-borne class C enzymes were identified in important clinical bacterial species that do not naturally produce significant amounts of these types of ␤-lactamase (such as Klebsiella pneumoniae or Escherichia coli) or do not possess an ampC gene (e.g., Salmonella spp.) (10, 34) and actually bear an increasing clinical importance. From a functional standpoint, class C ␤-lactamases are typically characterized by a very efficient hydrolysis of narrow-spectrum cephalosporins but are also able to degrade more recently found extended-spectrum cephalosporins and cephamycins and, thus, can confer resistance to these agents, especially in strains where the ␤-lactamase is derepressed or plasmid encoded (9,11,34).Aeromonads are ubiquitous waterborne organisms, and some Aeromona...

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Aeromonas hydrophila with Plasmid-Borne Class A Extended-Spectrum β-Lactamase TEM-24 and Three Chromosomal Class B, C, and D β-Lactamases, Isolated from a Patient with Necrotizing Fasciitis

Cited by 36 publications

References 13 publications

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

Genome Sequence of Aeromonas hydrophila ATCC 7966 ^T : Jack of All Trades

Genetic and Biochemical Characterization of TRU-1, the Endogenous Class C β-Lactamase from Aeromonas enteropelogenes

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Aeromonas hydrophila with Plasmid-Borne Class A Extended-Spectrum β-Lactamase TEM-24 and Three Chromosomal Class B, C, and D β-Lactamases, Isolated from a Patient with Necrotizing Fasciitis

Cited by 36 publications

References 13 publications

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

The GenusAeromonas: Taxonomy, Pathogenicity, and Infection

Genome Sequence of Aeromonas hydrophila ATCC 7966 T : Jack of All Trades

Genetic and Biochemical Characterization of TRU-1, the Endogenous Class C β-Lactamase from Aeromonas enteropelogenes

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Genome Sequence of Aeromonas hydrophila ATCC 7966 ^T : Jack of All Trades