<i>Aeromonas</i>spp. from marketed Yesso scallop (<i>Patinopecten yessoensis</i>): molecular characterization, phylogenetic analysis, virulence properties and antimicrobial susceptibility

Silva, B.C.J. De; Hossain, Sabrina; Dahanayake, P.S.; Heo, Gang‐Joon

doi:10.1111/jam.14106

Cited by 35 publications

(29 citation statements)

References 51 publications

(45 reference statements)

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“…2018; De Silva et al . 2019). High occurrence (68%) of A. hydrophila from retailed seafood products was reported in one German study (Ullmann et al .…”

Section: Discussionmentioning

confidence: 99%

Aeromonas spp. isolated from ready‐to‐eat seafood on the Norwegian market: prevalence, putative virulence factors and antimicrobial resistance

et al. 2020

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Aims We aim to investigate the prevalence, putative virulence factors and antimicrobial resistance of mesophilic Aeromonas isolated from ready‐to‐eat (RTE) seafood available on the Norwegian market, and to assess the potential risks by consuming RTE seafood to consumers. Methods and Results The prevalence of mesophilic Aeromonas in 148 RTE seafood was investigated and the highest prevalence was found in retail sushi (17%), followed by oysters (10%), fresh salmon loins (10%) and scallops (4%). Among 43 Aeromonas isolates, 75% of them were identified as A. media, 23% as A. salmonicida and 2% as A. bestiarum based on partial gryB gene sequencing. Aeromonas isolates were potentially pathogenic due to the presence of four virulence genes: alt (73%), hylA (22%), aerA (17%) and act (6%). In addition, all isolates were resistant to ampicillin and erythromycin. Most of the isolates (98%) were multidrug resistant. Conclusions The occurrence of potentially pathogenic and multidrug‐resistant Aeromonas strains in RTE seafood implies a potential risk to consumers. Our finding suggests that RTE seafood could be a potential vehicle for the transfer of virulent and multidrug‐resistant Aeromonas. Significance and Impact of the Study To our knowledge, this is the first study to report multiple antibiotic resistance in Aeromonas associated with RTE seafood in Norway.

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“…2018; De Silva et al . 2019). High occurrence (68%) of A. hydrophila from retailed seafood products was reported in one German study (Ullmann et al .…”

Section: Discussionmentioning

confidence: 99%

Aeromonas spp. isolated from ready‐to‐eat seafood on the Norwegian market: prevalence, putative virulence factors and antimicrobial resistance

et al. 2020

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“…Ramadan et al (2018) found the occurrence of aerA (70%), ast (46%), hlyA (42%) and act (32%) from 50 examined A. hydrophila isolates in marketed mullet. De Silva et al (2019) reported the detected frequency of aerA (78%), hlyA (75%), act (75%), alt (59%) and ast (47%) among 23 Aeromonas isolates from marketed scallops. All the results showed that the prevalence rates of the same virulence gene were different in different samples, which led to a variety of virulence gene combinations.…”

Section: Tissue Samplesmentioning

confidence: 99%

“…The most reviewed virulence factors of Aeromonas sp. are aerolysin (encoded by the gene aer , similar description is suitable for other virulence factors here‐in‐after), haemolysin ( hly ), enterotoxin including labile temperature cytotoxic enterotoxin ( alt ), stable temperature cytotoxic enterotoxin ( ast ) and cytotoxic enterotoxin ( act ), temperature‐sensitive protease ( epr ), extracellular serine protease ( ahp ), serine protease ( ser ), flagellin ( fla ), lipases ( lip ), elastase ( ela ), ADP‐ribosyltransferase toxin ( aex T) and DNases ( exu ) (Li et al ; Yi et al ; Jung‐Schroers et al ; De Silva et al ). To predict the virulence strength of Aeromonas strains and its clinical symptoms, the establishment of a rapid method to distinguish virulent and nonvirulent Aeromonas strains by screening major virulence factors is needed.…”

Section: Introductionmentioning

confidence: 99%

A novel multiplex PCR assay for rapid detection of virulent Aeromonas in cultured eels

Xiong

Huang

Guo

et al. 2019

J of Applied Microbiology

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Aims Under intensive and stressful aquaculture conditions, cultured eels are highly susceptible to virulent Aeromonas sp. infections. To rapidly and simultaneously confirm Aeromonas isolate and its virulence, a two‐tube multiplex PCR (mPCR) assay incorporating gyrB gene for genus‐specific recognition and seven major virulence genes for virulence assessment was developed. Methods and Results Eight pairs of primers were designed and divided into two groups—gyrB, ahpA, epr and aerA in tube 1 and alt, act, ast and hlyA in tube 2. The optimized mPCR conditions were the same except for their final concentrations. The specificity of the mPCR was validated by the extracted DNA of 10 Aeromonas and 8 non‐Aeromonas species, or mixed DNA templates. Detection limits were determined to be 200 copies per μl in tube 1 and 20 copies per μl in tube 2. The mPCR reproducibility was tested by both artificial challenge and clinical samples. Conclusions The results showed this two‐tube mPCR assay was rapid, specific, sensitive and reliable. Significance and Impact of the Study To our knowledge, this is the first report to distinguish virulent Aeromonas isolates from nonvirulent ones by seven popular and major virulence genes at the genus‐specific level. And it will be useful for large‐scale screening of virulent Aeromonas sp. in cultured eels.

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“…2015; De Silva et al . 2019). The genus Aeromonas consists of Gram negative, facultative anaerobic, rod‐shaped bacteria (Martin‐Carnahan and Joseph 2005).…”

Section: Introductionmentioning

confidence: 99%

Growth and spoilage metabolites production of a mesophilic Aeromonas salmonicida strain in Atlantic salmon ( Salmo salar L.) during cold storage in modified atmosphere

Jakobsen

Shumilina

Lied

et al. 2020

J. Appl. Microbiol.

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Aims The aim of the study was to quantify the growth kinetic parameters and spoilage‐associated metabolites of an inoculated strain of Aeromonas salmonicida in pre‐rigor filleted Atlantic salmon (Salmo salar L.) stored in vacuum (VP) or modified atmosphere (MAP 60/40% CO2/N2) at 4 and 8°C. Methods and Results The maximum growth rate of A. salmonicida in VP salmon stored at 4°C was 0·56 ± 0·04 day−1 with no detectable lag‐phase and the concentration of Aeromonas reached 8·33 log CFU per g after 10 days. The growth rates and maximum population density of Aeromonas in MAP salmon were lower but the applied atmosphere did not inhibit the growth. A selection of metabolites associated with fish spoilage were quantified using 1H nuclear magnetic resonance (NMR) spectroscopy. The concentration of trimethylamine (TMA) was significantly affected by storage time and temperature, packaging atmosphere and inoculation with A. salmonicida (General Linear Model (GLM), P < 0·001 for all factors). Conclusion The study presents preliminary results on A. salmonicida as a potential spoilage organism in vacuum‐packaged salmon during cold storage. The combination of refrigeration and a packaging atmosphere consisting of 60/40 % CO2/N2 did not completely inhibit the growth but prevented the formation of TMA. Significance and Impact of the Study Little information is available on the spoilage potential of Aeromonas spp. in minimally processed salmon products under different packaging conditions. The study clearly demonstrates the importance of hurdle technology and provides data to further elucidate the significance of Aeromonas spp. as a spoilage organism.

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Aeromonasspp. from marketed Yesso scallop (Patinopecten yessoensis): molecular characterization, phylogenetic analysis, virulence properties and antimicrobial susceptibility

Abstract: To our knowledge, this is the first study reporting Aeromonas spp. in scallop. This implies that not only the common varieties like oysters, but other bivalves can also harbour potentially pathogenic aeromonads which may have impacts on consumer health.

Cited by 35 publications

References 51 publications

Aeromonas spp. isolated from ready‐to‐eat seafood on the Norwegian market: prevalence, putative virulence factors and antimicrobial resistance

Aeromonas spp. isolated from ready‐to‐eat seafood on the Norwegian market: prevalence, putative virulence factors and antimicrobial resistance

A novel multiplex PCR assay for rapid detection of virulent Aeromonas in cultured eels

Growth and spoilage metabolites production of a mesophilic Aeromonas salmonicida strain in Atlantic salmon ( Salmo salar L.) during cold storage in modified atmosphere

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