<i>Agaricus bisporus</i>-derived β-glucan enter macrophages and adipocytes by CD36 receptor

Li, Xiumin; Zhang, Xiufen; Pang, Lei; Yao, Liyun; Shangguan, Zhaoshui; Pan, Yutian

doi:10.1080/14786419.2018.1556654

Cited by 12 publications

(10 citation statements)

References 12 publications

(1 reference statement)

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“…However, it is not clear if TLR2 is the specific β-glucan receptor because it is not normally expressed in the cells 18 . Interestingly, a recent study suggests the role of CD36, a scavenger receptor, as a β-glucan receptor in both macrophages and 3T3-L1 adipocytes 48 . Thus, future investigation is required to identify the specific β-glucan receptor on adipocytes.…”

Section: Discussionmentioning

confidence: 99%

Fungal-like particles and macrophage-conditioned medium are inflammatory elicitors for 3T3-L1 adipocytes

Jakkawanpitak

Hutadilok‐Towatana

Sermwittayawong

2020

Sci Rep

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fungal-like particles and macrophage-conditioned medium are inflammatory elicitors for 3T3-L1 adipocytes chanawee Jakkawanpitak, nongporn Hutadilok-towatana & Decha Sermwittayawong ✉ Adipocytes from white-adipose tissue are known to produce inflammatory cytokines, which play a major role in energy balance and metabolism. While they can respond to pathogen-associated molecular pattern (PAMPs) such as lipopolysaccharide (LPS) from bacteria, it is not known whether adipocytes can be stimulated by fungal cells. Previously, adipocytes were shown to produce toll-like receptor 2 (TLR2), a β-glucan receptor, suggesting that they could respond to β-glucan on the fungal cell wall. In this study, we show that heat-killed yeast induce an inflammatory response in adipocytes. Using fungal-like particles, namely laminarin-coated beads (LCB), we find that these particles trigger the expression of many key inflammatory genes in dose-and time-dependent fashions in adipocytes. these results suggest that β-glucan on the fungal cell wall is sufficient to elicit an inflammatory response in adipocytes. In addition, we show that both LCB and LCB-treated conditioned medium from RAW 264.7 murine macrophages (LCB-RM) induce the expression of those inflammatory genes through iKKβ-iκBα proteins. Together, we conclude that the fungal-like particles and the conditioned medium elicit an inflammatory response in adipocytes through the canonical or classical NF-κB pathway.

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Section: Discussionmentioning

confidence: 99%

Fungal-like particles and macrophage-conditioned medium are inflammatory elicitors for 3T3-L1 adipocytes

Jakkawanpitak

Hutadilok‐Towatana

Sermwittayawong

2020

Sci Rep

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“…Among the latter, Dectin-1 is the best characterized receptor, reported to bind β-glucan from different sources, and is expressed on the surface of monocytes, macrophages, neutrophils, DC and T lymphocytes [22]. Other PRR, including lactosylceramide receptor, mannose receptor, complement and scavenger receptors were reported to directly bind β-glucan or to cooperate with Dectin-1 for its recognition [15,21,23]. Recently, β-glucan was shown to stimulate NK cell cytotoxic activity through direct binding to the NKp30 activating receptor [24].…”

Section: Dietary β-Glucans: Main Features and Sourcesmentioning

confidence: 99%

Shaping the Innate Immune Response by Dietary Glucans: Any Role in the Control of Cancer?

Cornò

Gessani

Conti

2020

Cancers

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β-glucans represent a heterogeneous group of naturally occurring and biologically active polysaccharides found in many kinds of edible mushrooms, baker’s yeast, cereals and seaweeds, whose health-promoting effects have been known since ancient times. These compounds can be taken orally as food supplements or as part of daily diets, and are safe to use, nonimmunogenic and well tolerated. A main feature of β-glucans is their capacity to function as biological response modifiers, exerting regulatory effects on inflammation and shaping the effector functions of different innate and adaptive immunity cell populations. The potential to interfere with processes involved in the development or control of cancer makes β-glucans interesting candidates as adjuvants in antitumor therapies as well as in cancer prevention strategies. Here, the regulatory effects of dietary β-glucans on human innate immunity cells are reviewed and their potential role in cancer control is discussed.

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“…Interestingly, a recent report suggested cluster of differentiation 36 (CD36), a scavenger receptor, as a candidate for a b-glucan receptor on the surface of macrophages and differentiated adipocytes 17 . CD36 plays a major role as a receptor that mediates the uptake of fatty acids in skeletal muscles and adipose tissues 41 .…”

Section: Possible B-glucan Receptors On 3t3-l1 Adipocytesmentioning

confidence: 99%

“…Interestingly, TLR2 has been suggested as a receptor candidate for b-glucan in 3T3-L1 adipocytes due to its upregulation in the cells upon the treatment with lipopolysaccharide or LPS 15,16 . In addition to TLR2, a recent study suggested the CD36, a scavenger receptor, to be a b-glucan receptor on both macrophages and 3T3-L1 adipocytes 17 . Therefore, while the structure and function of b-glucan receptors in the immune cells have been well characterized, it remains unclear for what the b-glucan receptor is on the 3T3-L1 adipocytes.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of the Fungal-like Particles in the Inhibition of Adipogenesis in 3t3-l1 Adipocytes

Jakkawanpitak

Inafuku

Oku

et al. 2021

Preprint

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The dynamic ability of adipocytes in adipose tissue to store lipid in response to changes in the nutritional input and inflammatory elicitors has a major impact on human health. Previously, we established laminarin-coated beads or LCB as an inflammatory elicitor for adipocytes. However, it was not clear whether LCB inhibits lipid accumulation in adipocytes. Here, we show that LCB acts in the early stage of adipogenesis through both IRAK and SYK pathways, resulting in the activation of the AMPK and NF-kB complexes, which subsequently cause cell cycle arrest, suppression of C/EBPb, PPARg, C/EBPa, FAS, FABP4, and ACC proteins, downregulation of other transcription factors and enzymes, such as Pparg, C/ebpa, Srebp-1, Lpl, and Fas gene expression, inhibition of adipogenesis, and stimulation of an inflammatory response. Unlike the inhibition of adipogenesis, LCB could stimulate an inflammatory response at any stage of differentiation. In addition, we find that Tlr2 and Clec7a/Dectin-1 but not Tlr4 and Cd36 gene expression are upregulated upon the treatment with LCB, suggesting that TLR2 and CLEC7A/Dectin-1 might be the b-glucan receptors for the cells. Together, we present the mechanism of LCB, as fungal-like particles, that elicit an inflammatory response and inhibit adipogenesis at the early stage of differentiation.

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Agaricus bisporus-derived β-glucan enter macrophages and adipocytes by CD36 receptor

Cited by 12 publications

References 12 publications

Fungal-like particles and macrophage-conditioned medium are inflammatory elicitors for 3T3-L1 adipocytes

Fungal-like particles and macrophage-conditioned medium are inflammatory elicitors for 3T3-L1 adipocytes

Shaping the Innate Immune Response by Dietary Glucans: Any Role in the Control of Cancer?

Mechanism of the Fungal-like Particles in the Inhibition of Adipogenesis in 3t3-l1 Adipocytes

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