<i>Aggregatibacter actinomycetemcomitans</i> Growth in Biofilm versus Planktonic State: Differential Expression of Proteins

Llama‐Palacios, Arancha; Potupa, Oksana; Sánchez, María Carmen; Figuero, Elena; Herrera, David; Sanz, Mariano

doi:10.1021/acs.jproteome.7b00127

Cited by 22 publications

(21 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Differential gene expressions between A. actinomycetemcomitans in its planktonic and biofilm states had been observed previously [44], and our study confirmed their observations on several genes. The gene PTS mannose transporter subunit IIAB (D7S_01753) was found to be upregulated by both studies.…”

Section: Discussionsupporting

confidence: 92%

Transcriptomic Analysis of Aggregatibacter actinomycetemcomitans Core and Accessory Genes in Different Growth Conditions

et al. 2019

View full text Add to dashboard Cite

Aggregatibacter actinomycetemcomitans genome can be divided into an accessory gene pool (found in some but not all strains) and a core gene pool (found in all strains). The functions of the accessory genes (genomic islands and non-island accessory genes) are largely unknown. We hypothesize that accessory genes confer critical functions for A. actinomycetemcomitans in vivo. This study examined the expression patterns of accessory and core genes of A. actinomycetemcomitans in distinct growth conditions. We found similar expression patterns of island and non-island accessory genes, which were generally lower than the core genes in all growth conditions. The median expression levels of genomic islands were 29%–37% of the core genes in enriched medium but elevated to as high as 63% of the core genes in nutrient-limited media. Several putative virulence genes, including the cytolethal distending toxin operon, were found to be activated in nutrient-limited conditions. In conclusion, genomic islands and non-island accessory genes exhibited distinct patterns of expression from the core genes and may play a role in the survival of A. actinomycetemcomitans in nutrient-limited environments.

show abstract

Section: Discussionsupporting

confidence: 92%

Transcriptomic Analysis of Aggregatibacter actinomycetemcomitans Core and Accessory Genes in Different Growth Conditions

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Therefore, it is a useful tool to study how A. actinomycetemcomitans orchestrates proteomic changes in the context of biofilms. Using 2D gels, Llama-Palacios et al discovered 87 protein spots differently expressed (1.5-fold, p < 0.05) between planktonic and mono-species biofilm cultures of A. actinomycetemcomitans [171]. Then, using MALDI-TOF MS, 13 upregulated proteins (from 24 proteins spots) and 37 downregulated proteins (from 50 spots) were identified.…”

Section: Localization In Biofilms and Proteomic Interactions With Othmentioning

confidence: 99%

Virulence and Pathogenicity Properties of Aggregatibacter actinomycetemcomitans

et al. 2019

View full text Add to dashboard Cite

Aggregatibacter actinomycetemcomitans is a periodontal pathogen colonizing the oral cavity of a large proportion of the human population. It is equipped with several potent virulence factors that can cause cell death and induce or evade inflammation. Because of the large genetic diversity within the species, both harmless and highly virulent genotypes of the bacterium have emerged. The oral condition and age, as well as the geographic origin of the individual, influence the risk to be colonized by a virulent genotype of the bacterium. In the present review, the virulence and pathogenicity properties of A. actinomycetemcomitans will be addressed.

show abstract

“…These outer membrane vesicles contain many effector proteins that are internalized into human host cells. 67,73 including the ability to attain a more realistic representation of the cell state, 76 the ability to connect various pathways via the observation of pathway intermediates, [84][85][86] and an unbiased evaluation of the cell state under specific conditions. 87,88 Monitoring metabolite profiles in body fluids to understand disease etiology has been a common focus of this approach.…”

Section: Proteomic In Vitro Studiesmentioning

confidence: 99%

Host‐microbe interactions: Profiles in the transcriptome, the proteome, and the metabolome

Nguyen

Sedghi

Ganther

et al. 2019

Periodontology 2000

View full text Add to dashboard Cite

Periodontal studies using transcriptomics, proteomics, and metabolomics encompass the collection of mRNA transcripts, proteins, and small‐molecule chemicals in the context of periodontal health and disease. The number of studies using these approaches has significantly increased in the last decade and they have provided new insight into the pathogenesis and host‐microbe interactions that define periodontal diseases. This review provides an overview of current molecular findings using ‐omic approaches that underlie periodontal disease, including modulation of the host immune response, tissue homeostasis, and complex metabolic processes of the host and the oral microbiome. Integration of these ‐omic approaches will broaden our perspective of the molecular mechanisms involved in periodontal disease, advancing and improving the diagnosis and treatment of various stages and forms of periodontal disease.

show abstract

Aggregatibacter actinomycetemcomitans Growth in Biofilm versus Planktonic State: Differential Expression of Proteins

Cited by 22 publications

References 72 publications

Transcriptomic Analysis of Aggregatibacter actinomycetemcomitans Core and Accessory Genes in Different Growth Conditions

Transcriptomic Analysis of Aggregatibacter actinomycetemcomitans Core and Accessory Genes in Different Growth Conditions

Virulence and Pathogenicity Properties of Aggregatibacter actinomycetemcomitans

Host‐microbe interactions: Profiles in the transcriptome, the proteome, and the metabolome

Contact Info

Product

Resources

About