Aggregatibacter actinomycetemcomitans serotypes associate with periodontal and coronary artery disease status

Abstract

Aggregatibacter actinomycetemcomitans serotypes "b" and "c" associate with both periodontal and CAD status. Detectable serotypes associate with the quantity and the serology of the bacterium emphasizing both local and systemic effect of the A. actinomycetemcomitans serotypes.

Mediators between oral dysbiosis and cardiovascular diseases

“…We recently showed that serotypes b and c were more frequently recognized in patients with CAD than in the non‐CAD reference group and that those serotypes were associated with increased risk of stable CAD. Both the quantities of bacteria and serum antibody levels against A. actinomycetemcomitans were highest in patients harbouring serotype c, and the lowest antibody levels were detected in patients with serotype a . It has also been shown, in vitro, that LPS from different strains of A. actinomycetemcomitans differ in their proatherogenic properties , further supporting the idea of the diverse virulence potential among A. actinomycetemcomitans serotypes.…”

Serum lipopolysaccharide neutralizing capacity in ischemic stroke

“…The amounts of A. actinomycetemcomitans and Porphyromonas gingivalis were determined with qPCR as previously described [28,34]. The serotype of A. actinomycetemcomitans in saliva of the bacterium-positive participants was further examined with A. actinomycetemcomitans serotype-specific qPCR assay as previously described [14] with minor modifications. qPCR reactions with total volume of 20 μl contained 1× Kapa SYBR Fast Universal qPCR master mix (Kapa Biosystems, Merck) supplemented with ROX reference dye, 2 μl of salivary DNA and 0.2 μM forward and reverse primers specific to A. actinomycetemcomitans serotypes A-E [14].…”

“…The serotype of A. actinomycetemcomitans in saliva of the bacterium-positive participants was further examined with A. actinomycetemcomitans serotype-specific qPCR assay as previously described [14] with minor modifications. qPCR reactions with total volume of 20 μl contained 1× Kapa SYBR Fast Universal qPCR master mix (Kapa Biosystems, Merck) supplemented with ROX reference dye, 2 μl of salivary DNA and 0.2 μM forward and reverse primers specific to A. actinomycetemcomitans serotypes A-E [14]. Each qPCR plate included a 10-fold dilution series of standard DNA extracted from a reference strain representing particular serotype (ATCC 29523 = serotype A, ATCC 43718 = serotype B, ATCC 33384 = serotype C, IDH 781 = serotype D, IDH 1705 = serotype E).…”

“…The serotypes of this species are characterized by structural differences in the Oantigen of LPS [13]. Diverse serotypes may differ in immunogenicity, which probably plays a role in both periodontitis and cardiovascular diseases [14].…”

Melatonin ameliorates periodontitis‐related inflammatory stress at cardiac left ventricular tissues in rats

“…Today, we have much evidence relating periodontal diseases with the pathogenesis of atherosclerotic cardiovascular diseases that can cause death by heart attack or advanced heart failure. [1][2][3][4] Present evidence shows that periodontal bacteria contribute to the initiation or advancement of the atherosclerotic changes on the vascular structures directly and/or by stimulating host immuno-inflammatory response. 2 Among the possible mechanisms, shown to be the most prevalent are; change in T-cell response, triggering endothelial dysfunction, modification of low-density lipoproteins and stimulation of maturation and activity of monocytes.…”