<i>Agrobacterium tumefaciens</i>: a natural tool for plant transformation

Riva, G; Gonzalez-Cabrera, Joel; Vázquez-Padrón, Roberto I.; Ayra‐Pardo, Camilo

doi:10.2225/vol1-issue3-fulltext-1

Cited by 69 publications

(41 citation statements)

References 84 publications

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“…In present study, a negative correlation of survival frequencies was observed with increase in hydrogen ion concentration in the media (low pH) on both selection as well regeneration media. In other studies, it was suggested that acidic pH of the infection medium enhanced the transformation frequency (De La Riva et al, 1998;Saini and Jaiwal., 2007).…”

Section: Ph Of the Infection Mediummentioning

confidence: 95%

Resourceful and High Efficiency Agrobacterium Mediated Transformation of Maize (Zea mays L.) Using Coleoptilar Nodal Explants

Sreenu¹,

Kumar²,

Reddy³

et al. 2016

Int. J. Curr. Res. Biosci. Plant Biol.

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A b s t r a c t A r t i c l e I n f oColeoptilar nodal explants, which are independent of season, were used to develop high efficiency transformation method in maize. Conditions which affect Agrobacterium-mediated genetic transformation have been standardized by using these explants on hygromycin selection regime. Hyper virulent Agrobacterium strain EHA105 containing gateway vector pMDC99 with Ascorbate -Glutathione pathway coding genes harboring with hygromycin phosphotransferase (hpt II) and bar as plant selectable marker genes was used. The survival frequency of calli at three different stages viz., at the end of I sub-culture on normal medium and II selection as well as on regeneration media supplemented with hygromycin were taken into consideration for the assessment of optimal conditions after checking with PCR. Of the different parameters used with different conditions, one day pre-conditioning of explants, 0.8 optical density of bacterial culture in plain MS liquid infection medium having 5.8 pH along with 200 µM acetosyrengone, 15 minutes infection time for the explants after treating with 0.1% Macerozyme, application of vacuum infiltration for 10 minutes, three days of co-cultivation period were found to be optimal for getting high frequency of transformation through Agrobacterium-mediated genetic transformation in maize. Transformation frequency of 3.25% was obtained on average with these optimal conditions, for which the explants were co-cultivated with these conditions and inoculated on callus induction medium having hygromycin at 10 mg l -1 for selection. The selected calli were transferred onto regeneration medium supplemented with BAP and Kinetin, at a concentration of 1 mg l -1 each for plantlet development. Putative transformants were screened by using hpt II gene specific primers and confirmed after performing southern analysis.

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Section: Ph Of the Infection Mediummentioning

confidence: 95%

Resourceful and High Efficiency Agrobacterium Mediated Transformation of Maize (Zea mays L.) Using Coleoptilar Nodal Explants

Sreenu¹,

Kumar²,

Reddy³

et al. 2016

Int. J. Curr. Res. Biosci. Plant Biol.

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“…In addition, predictable and stable expression of transgenes remains problematic. Several excellent reviews have appeared recently that describe in detail various aspects of Agrobacterium biology (44,73,109,325,327,328,384,385). In this review, I describe how scientists utilized knowledge of basic Agrobacterium biology to develop Agrobacterium as a "tool" for plant genetic engineering.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Gelvin

2003

Microbiol Mol Biol Rev

1,167

687

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SUMMARY Agrobacterium tumefaciens and related Agrobacterium species have been known as plant pathogens since the beginning of the 20th century. However, only in the past two decades has the ability of Agrobacterium to transfer DNA to plant cells been harnessed for the purposes of plant genetic engineering. Since the initial reports in the early 1980s using Agrobacterium to generate transgenic plants, scientists have attempted to improve this “natural genetic engineer” for biotechnology purposes. Some of these modifications have resulted in extending the host range of the bacterium to economically important crop species. However, in most instances, major improvements involved alterations in plant tissue culture transformation and regeneration conditions rather than manipulation of bacterial or host genes. Agrobacterium-mediated plant transformation is a highly complex and evolved process involving genetic determinants of both the bacterium and the host plant cell. In this article, I review some of the basic biology concerned with Agrobacterium-mediated genetic transformation. Knowledge of fundamental biological principles embracing both the host and the pathogen have been and will continue to be key to extending the utility of Agrobacterium for genetic engineering purposes.

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“…Plant growth regulators and types of explant are the most important factors for callus induction and shoot regeneration phases (Mineo, 1990). Transformation, by means of Agrobacterium-mediated or direct gene transfer methods is a widely-used technique for genetic improvement of crops (de la Riva, 1998). The transformation techniques are essentially based on callus formation and regeneration of transformed calli.…”

Section: Discussionmentioning

confidence: 99%

Regeneration ability and genetic transformation of root type chicory (Cichorium intybus var. sativum)

Maroufi¹

2012

Afr. J. Biotechnol.

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Agrobacterium tumefaciens: a natural tool for plant transformation

Cited by 69 publications

References 84 publications

Resourceful and High Efficiency Agrobacterium Mediated Transformation of Maize (Zea mays L.) Using Coleoptilar Nodal Explants

Resourceful and High Efficiency Agrobacterium Mediated Transformation of Maize (Zea mays L.) Using Coleoptilar Nodal Explants

Agrobacterium -Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Regeneration ability and genetic transformation of root type chicory (Cichorium intybus var. sativum)

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