<i>Agrobacterium tumefaciens</i>
            Is a Diazotrophic Bacterium

Kanvinde, Lalita; Sastry, G. R. K.

doi:10.1128/aem.56.7.2087-2092.1990

Cited by 47 publications

(18 citation statements)

References 18 publications

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“…The amplification of part of the nifH gene was also successfully carried out for Agrobacterium tumefaciens C58, which displayed a band corresponding to the fragment observed for E. cloacae. These results confirm at the molecular level recent reports on the diazotrophic status of A. tumefaciens C58 (18).…”

Section: Fgph273'supporting

confidence: 91%

Frankia genus-specific characterization by polymerase chain reaction

Simonet

Grosjean

Misra

et al. 1991

Appl Environ Microbiol

134

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The polymerase chain reaction (PCR) is an in vitro procedure for primer-directed enzymatic amplification of specific template nucleic acid sequences. In order to determine whether a given actinomycete isolated from an actinorhiza (nodule) belongs to the genus Frankia or is a contaminant, we have developed a test based on the PCR. Primers complementary to sequences of two DNA regions corresponding to the nif genes (nifH and nifD) and the rRNA genes (16S and 23S) were specifically chosen to differentially amplify DNAs from Frankia strains but not those from other microorganisms. A series of positive and negative controls were set up by using universal or selective primers resulting in a discriminant amplification, which could be detected after agarose gel electrophoresis. In the nif region, degenerate oligonucleotide primers were used to amplify a target common to all the nitrogen-fixing microorganisms tested, while another set of primers amplified a target with a high specificity for Frankia strains. In the rRNA gene region, universal and specific primers were characterized and tested with DNAs from a wide range of microorganisms. The efficiency of this rapid and sensitive PCR assay was tested with an isolate obtained from Alnus nepalensis nodules, confirming results obtained by nodulation tests.

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Section: Fgph273'supporting

confidence: 91%

Frankia genus-specific characterization by polymerase chain reaction

Simonet

Grosjean

Misra

et al. 1991

Appl Environ Microbiol

134

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“…This result is surprising since the DNA sequence of nifH is highly conserved among nitrogen-fixers (Ruvkun & Auzubel 1980;Normand et al 1988) and a Klebsiella pneumoniae nifHDK probe has frequently been used to detect homologous genes in very distant organisms. However, diazotrophic strains of Agrobacterium (Kanvinde & Sastry 1990) and of Methylosinus (Oakley & Murrel1988) were found to lack homology to the Klebsiella nif gene probe. It should be also noted that the bean isolates of group E formed symbiotically ineffective nodules on both P. vulgaris and L. leucocephala (Laguerre et al 1993;N.…”

Section: Comparison Of Populations Of Rhizobium 301mentioning

confidence: 99%

Comparison of geographically distant populations of Rhizobium isolated from root nodules of Phaseolus vulgaris

1993

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Seventy-two rhizobial strains were isolated from the root nodules of french beans (Phaseolus vulgaris). They were sampled from two geographically distant field populations and 18 additional different sites in France. They were characterized by a) plasmid profiles, (b) RFLP analysis of total cellular DNA using various chromosomal and symbiotic gene probes (including nifH from Rhizobium etli bv. phaseoli) and c) their ability to nodulate a potential alternative host, L. leucocephala. Over half of the isolates were ascribed to Rhizobium leguminosarum bv. phaseoli on the basis of the hybridization analysis, the possession of multiple copies of n i p and their inability to nodulate L. leucocephala. The remaining isolates belonged to 2 groups which were shown to be genomically distinct from R. leguminosarum bv. phaseoli, R. etli bv. phaseoli and R. tropici. Most members of these two groups shared with R. tropici the ability to nodulate L. leucocephala and, for isolates of only one of these groups, the presence of one copy of n i p . Members of each of the 3 taxa were widely distributed in France and circumstantial evidence of pSym transfer between them was shown. R. leguminosarum bv. phaseoli and one of the two novel groups co-occurred within the two geographically distant populations. Individual genotypes were conserved between them. The finding of a third taxon at various other locations indicated additional diversity among rhizobia nodulating beans.

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“…In this investigation, a structure resembles to root nodule often found in Leguminaceae plant roots was observed and assumed similar to typical nitrogen-fixing root nodules produced by Rhizobium sp. A few literatures reported that although A. tumefaciens is known as pathogenic bacterium for crown gall formation in roots, this bacterium was also found as similar to diazotrophs and report that A. tumefaciens can fix nitrogen and are capable of growing on nitrogen-free medium [47, 48]. However, the our finding is the first report as per current knowledge that shows A. tumefaciens can produce some sort of root nodules type structure with Vachellia pachyceras roots and have the ability to grow on nitrogen-free medium and reduce acetylene to ethylene at low level.…”

Section: Discussionmentioning

confidence: 99%

Divulging diazotrophic bacterial community structure in Kuwait desert ecosystems and their N2-fixation potential

Suleiman

Quoreshi

Bhat

et al. 2019

Preprint

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2 24 Abstract 25 Kuwait is a semi-arid region with harsh climatic conditions with poor available soil nutrient 26 essential for the growth of plants. Kuwait's ecosystem is relatively N-poor ecosystem when 27 compared to the other ecosystems. Biological nitrogen fixation (BNF) is a spontaneous process in 28 which diazotrophic bacteria fixes the atmospheric nitrogen directly in to the bionetwork. At 29 present, there is limited information on free-living and root associated nitrogen-fixing bacteria, 30 their potential to fix nitrogen to aid natural plant communities in the desert of Kuwait. In this study, 31 free-living N 2 -fixing bacteria were enriched and isolated from the rhizospheric soil of three 32 keystone native plant species of Kuwait; such as Rhanterium epapposum, Farsetia aegyptia, and 33 Haloxylon salicornicum. Root associated bacteria were directly isolated from the root nodules of 34 Vachellia pachyceras. In this study, a number of free-living and root associated dizotrophs were 35 isolated from various rhizospheric soils of three native shrubs and root nodules from one tree 36 species. The screened isolates were assessed for nitrogen-fixing ability and identified using 37 Acetylene Reduction Assay (ARA) and 16s rRNA gene sequencing, respectively. Our study 38 successfully identified all the 50 nitrogen-fixers isolated initially and out of that, 78% were 39 confirmed as nitrogen-fixers using ARA. Among the identified nitrogen fixers, the genus 40 Rhizobium is dominant in rhizospheric soil of Rhanterium epapposum, whereas Pseudomonas and 41 Rhizobium are dominant in the rhizospheric soil of Farsetia aegyptia, and Haloxylon salicornicum 42 respectively. The species Agrobacterium tumefaciens is found dominant in the root nodules of V.43 pachyceras. The current results indicate that plant species and their rhizospheric effects are 44 important drivers for specificity of microbial diversity in arid soils. To our knowledge, this study 45 is the first investigation of culture-based isolation, molecular identification, and evaluation of N 2 -46 fixing ability of diazotrophs from Kuwait desert environment. 3 47 Introduction 48 Symbiotic bacterial association with plants mediates the most of the nitrogen fixation process in 49 terrestrial ecosystems. Nevertheless, non-symbiotic N 2 fixation by free-living nitrogen-fixing 50 bacteria harboring in soil systems can considerably contribute to the nitrogen fixation pool in 51 various ecosystems, particularly in desert ecosystems [1, 2]. Nitrogen is an essential element 52 required for plant growth and development, considered a limiting factor in plant productivity [3, 53 4] and can affect life of microbes and other living organisms [5]. Nitrogen is an important 54 component of amino acids, the building blocks of proteins; chlorophyll, the green pigment required 55 for photosynthesis; ATP, the primary energy carrier; nucleic acid, the genetic material of living 56 organisms [6]. Thus, nitrogen plays a fundamental role in the growth and productivity of crops.57 Nitro...

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Agrobacterium tumefaciens Is a Diazotrophic Bacterium

Cited by 47 publications

References 18 publications

Frankia genus-specific characterization by polymerase chain reaction

Frankia genus-specific characterization by polymerase chain reaction

Comparison of geographically distant populations of Rhizobium isolated from root nodules of Phaseolus vulgaris

Divulging diazotrophic bacterial community structure in Kuwait desert ecosystems and their N2-fixation potential

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