Agromyces silvae sp. nov., Rathayibacter soli sp. nov., and Nocardioides terrisoli sp. nov., Isolated from Soil

Abstract

Three Gram-stain-positive, aerobic, rod-shaped, and non-motile bacteria, labelled as W11 T , SW19 T , and YR1 T , were isolated from soil, and performed their polyphasic taxonomic investigation. The phylogenetic and 16S rRNA gene sequence analysis showed that strains W11 T , SW19 T , and YR1 T belonged to the genera Agromyces , Rathayibacter , and Nocardioides , respectively. Strain W11 T was closely affiliated with Agromyces cavernae SYSU K20354 T (98.1%), strain SW19 T showed the closest affiliation with Rathayibacter rubneri ZW T2_19 T (97.0%), and strain YR1 T was most closely related to Nocardioides marmorisolisilvae KIS18-7 T (98.0%). The genome sizes of strains W11 T , SW19 T , and YR1 T were 4,181,720 bp, 4,740,677 bp, and 4,228,226 bp, respectively, with DNA G+C contents of 70.5%, 64.2%, and 69.7%, respectively. Average nucleotide identity and digital DNA–DNA hybridization values of W11 T , SW19 T , and YR1 T with their respective reference species were <79.6% and <23.6%, respectively. The predominant cellular fatty acids detected in strain W11 T were anteiso-C 15:0 , iso-C 16:0 , and anteiso-C 17:0 . In strain SW19 T , they were summed feature 9 (C 16:0 10-methyl and/or iso-C 17:1 ω 9c), anteiso-C 17:0 , and anteiso-C 15:0 . Strain YR1 T exhibited C 18:1 ω 9c, C 18:0 10-methyl, TBSA, and anteiso-C 15:0 as its major cellular fatty acids. Overall, the polyphasic taxonomic comparisons indicated that strains W11 T , SW19 T , and YR1 T represent novel species within the genera Agromyces , Rathayibacter , and Nocardioides , respectively. Accordingly, we propose the names Agromyces silvae sp. nov., with the type strain W11 T (=KCTC 49818 T =NBRC 115999 T ), Rathayibacter soli sp. nov., with the type strain SW19 T (=KCTC 49860 T =NBRC 116108 T ), and Nocardioides terrisoli sp. nov., with the type strain YR1 T ...