<i>AGT1</i>
            , Encoding an α-Glucoside Transporter Involved in Uptake and Intracellular Accumulation of Trehalose in
            <i>Saccharomyces cerevisiae</i>

Plourde-Owobi, Lucile; Durner, Sophie; Parrou, Jean-Luc; Wieczorke, Roman; Goma, G.; François, Jean

doi:10.1128/jb.181.12.3830-3832.1999

Cited by 32 publications

(11 citation statements)

References 24 publications

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“…In S. cerevisiae, Agt1p (also called Mal11p) is a high-affinity H ϩ -trehalose symporter (152). The deletion of this gene causes decreased tolerance to peroxide and heat shock (153).…”

Section: Trehalose Transportersmentioning

confidence: 99%

Central Role of the Trehalose Biosynthesis Pathway in the Pathogenesis of Human Fungal Infections: Opportunities and Challenges for Therapeutic Development

Thammahong

Puttikamonkul

Perfect

et al. 2017

Microbiol Mol Biol Rev

101

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Invasive fungal infections cause significant morbidity and mortality in part due to a limited antifungal drug arsenal. One therapeutic challenge faced by clinicians is the significant host toxicity associated with antifungal drugs. Another challenge is the fungistatic mechanism of action of some drugs. Consequently, the identification of fungus-specific drug targets essential for fitness remains a significant goal of medical mycology research. The trehalose biosynthetic pathway is found in a wide variety of organisms, including human-pathogenic fungi, but not in humans. Genes encoding proteins involved in trehalose biosynthesis are mechanistically linked to the metabolism, cell wall homeostasis, stress responses, and virulence of, , and. While there are a number of pathways for trehalose production across the tree of life, the TPS/TPP (trehalose-6-phosphate synthase/trehalose-6-phosphate phosphatase) pathway is the canonical pathway found in human-pathogenic fungi. Importantly, data suggest that proteins involved in trehalose biosynthesis play other critical roles in fungal metabolism and fitness that remain to be fully elucidated. By further defining the biology and functions of trehalose and its biosynthetic pathway components in pathogenic fungi, an opportunity exists to leverage this pathway as a potent antifungal drug target. The goal of this review is to cover the known roles of this important molecule and its associated biosynthesis-encoding genes in the human-pathogenic fungi studied to date and to employ these data to critically assess the opportunities and challenges facing development of this pathway as a therapeutic target.

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“…In S. cerevisiae, Agt1p (also called Mal11p) is a high-affinity H ϩ -trehalose symporter (152). The deletion of this gene causes decreased tolerance to peroxide and heat shock (153).…”

Section: Trehalose Transportersmentioning

confidence: 99%

Central Role of the Trehalose Biosynthesis Pathway in the Pathogenesis of Human Fungal Infections: Opportunities and Challenges for Therapeutic Development

Thammahong

Puttikamonkul

Perfect

et al. 2017

Microbiol Mol Biol Rev

101

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“…Unlike Mal61p which is speci¢c for maltose uptake, Agt1p can also actively transport other K-gluco-sides including trehalose, turanose, isomaltose, K-methylglucoside and maltotriose [119]. The proposed function of Agt1p to actively transport trehalose from the medium [118] was genetically con¢rmed by showing that a maltose-positive yeast strain defective in trehalose synthase subunit (tps1 mutant) no longer accumulates trehalose upon deletion of AGT1 in the presence of a low concentration of the disaccharide ( 6 4 mg ml 31 ) in the medium [122]. However, measurable intracellular trehalose in a agt1vtps1v double mutant cultivated with a high exogenous concentration of the disaccharide ( s 10 mg ml 31 ) supports the existence of a low a¤nity trehalose uptake.…”

Section: Trehalose Transportmentioning

confidence: 99%

Reserve carbohydrates metabolism in the yeast Saccharomyces cerevisiae

François¹

2001

FEMS Microbiology Reviews

323

368

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Glycogen and trehalose are the two glucose stores of yeast cells. The large variations in the cell content of these two compounds in response to different environmental changes indicate that their metabolism is controlled by complex regulatory systems. In this review we present information on the regulation of the activity of the enzymes implicated in the pathways of synthesis and degradation of glycogen and trehalose as well as on the transcriptional control of the genes encoding them. cAMP and the protein kinases Snf1 and Pho85 appear as major actors in this regulation. From a metabolic point of view, glucose-6-phosphate seems the major effector in the net synthesis of glycogen and trehalose. We discuss also the implication of the recently elucidated TOR-dependent nutrient signalling pathway in the control of the yeast glucose stores and its integration in growth and cell division. The unexpected roles of glycogen and trehalose found in the control of glycolytic flux, stress responses and energy stores for the budding process, demonstrate that their presence confers survival and reproductive advantages to the cell. The findings discussed provide for the first time a teleonomic value for the presence of two different glucose stores in the yeast cell.

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“…To explain this ¢nding a biosynthetic pathway for trehalose using adenine-diphospho-glucose instead of uridinediphospho-glucose was proposed [10]. However, a gene encoding such activity has not yet been identi¢ed and the trehalose accumulation seems to be due to its uptake from complex media by a broad-speci¢city transporter encoded by the AGT1 gene [11].…”

Section: The Biosynthesis Of Trehalose In Yeastsmentioning

confidence: 99%

The importance of a functional trehalose biosynthetic pathway for the life of yeasts and fungi

Gancedo

Flores

2004

FEMS Yeast Research

199

148

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The view of the role of trehalose in yeast has changed in the last few years. For a long time considered a reserve carbohydrate, it gained new importance when its function in the acquisition of thermotolerance was demonstrated. More recently the cellular processes in which the trehalose biosynthetic pathway has been implicated range from the control of glycolysis to sporulation and infectivity by certain fungal pathogens. There is now enough experimental evidence to conclude that trehalose 6-phosphate, an intermediate of trehalose biosynthesis, is an important metabolic regulator in such different organisms as yeasts or plants. Its inhibition of hexokinase plays a key role in the control of the glycolytic flux in Saccharomyces cerevisiae but other, likely important, sites of action are still unknown. We present examples of the phenotypes produced by mutations in the two steps of the trehalose biosynthetic pathway in different yeasts and fungi, and whenever possible examine the molecular explanations advanced to interpret them.

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AGT1 , Encoding an α-Glucoside Transporter Involved in Uptake and Intracellular Accumulation of Trehalose in Saccharomyces cerevisiae

Cited by 32 publications

References 24 publications

Central Role of the Trehalose Biosynthesis Pathway in the Pathogenesis of Human Fungal Infections: Opportunities and Challenges for Therapeutic Development

Central Role of the Trehalose Biosynthesis Pathway in the Pathogenesis of Human Fungal Infections: Opportunities and Challenges for Therapeutic Development

Reserve carbohydrates metabolism in the yeast Saccharomyces cerevisiae

The importance of a functional trehalose biosynthetic pathway for the life of yeasts and fungi

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