2021
DOI: 10.1002/advs.202002794
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AKAP1 Deficiency Attenuates Diet‐Induced Obesity and Insulin Resistance by Promoting Fatty Acid Oxidation and Thermogenesis in Brown Adipocytes

Abstract: Altering the balance between energy intake and expenditure is a major strategy for treating obesity. Nonetheless, despite the progression in antiobesity drugs on appetite suppression, therapies aimed at increasing energy expenditure are limited. Here, knockout ofAKAP1, a signaling hub on outer mitochondrial membrane, renders mice resistant to diet‐induced obesity.AKAP1 knockout significantly enhances energy expenditure and thermogenesis in brown adipose tissues (BATs) of obese mice. Restoring AKAP1 expression … Show more

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Cited by 20 publications
(20 citation statements)
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“…HCC tissues and cell lines were processed for Western blot as previously described (Ji et al , 2021). Western blot was then analyzed for band intensity with ImageJ software (National Institutes of Health, MD).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…HCC tissues and cell lines were processed for Western blot as previously described (Ji et al , 2021). Western blot was then analyzed for band intensity with ImageJ software (National Institutes of Health, MD).…”
Section: Methodsmentioning
confidence: 99%
“…LifeAct‐mCherry transduction was also performed using a customized retrovirus (Genepharma, Shanghai, China) according to the supplier’s instructions, by addition of HiTransG (GeneChem, Shanghai, China), a viral transduction reagent, to achieve a MOI of 200 IU/cell. Immunofluorescence was performed as described previously (Ji et al , 2021). Then, the HCC cells and tissues were viewed using an Olympus FV3000 laser‐scanning confocal microscope (Tokyo, Japan).…”
Section: Methodsmentioning
confidence: 99%
“…Furthermore, the progressive accumulation of Annexin A2 (AnxA2) in the myofiber matrix causes muscle-resident fibro/adipogenic precursors (FAP) differentiation into adipocytes, and depletion of AnxA2 prevents FAP adipogenesis and muscle loss [ 62 ]. ACSL1 is required for beta oxidation in adipocytes, and it is associated to adipose insulin sensitivity, adipogenesis and adipocyte fatty acid uptake [ 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 ]. ELAVL1 (also named HuR) is an important repressor of adipogenesis.…”
Section: Resultsmentioning
confidence: 99%
“…We recently demonstrated that AKAP1 could recruit dynaminrelated protein 1 (Drp1) to promote mitochondrial fission, causing mitochondrial dysfunction and podocyte injury in DKD [4,50]. AKAP1 deletion attenuated diet-induced obesity and insulin resistance by promoting fatty acid oxidation and thermogenesis in brown adipocytes [51]. AKAP1 interacts with the NADH-ubiquinone oxidoreductase subunit to maintain mitochondrial complex Ⅰ activity and ATP production [52].…”
Section: Discussionmentioning
confidence: 99%
“…Akinase anchoring protein 1 (AKAP1) is an anchoring protein localized on the outer mitochondrial membrane (OMM), that recruits various molecules to regulate mitochondrial functions, including mitochondrial metabolism and dynamics [15,16]. AKAP1 plays an important role in mitochondrial biogenesis and function.…”
Section: Ivyspring International Publishermentioning
confidence: 99%