<i>Akkermansia muciniphila</i> and its outer protein Amuc_1100 regulates tryptophan metabolism in colitis

Gu, Zhenyang; Pei, Wenlong; Shen, Yonghua; Wang, Limin; Zhu, Jun; Zhang, Yi; Fan, Shiqing; Wu, Qian; Li, Lei; Zhang, Zhan

doi:10.1039/d1fo02172a

Cited by 81 publications

(54 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A. muciniphila has been shown to influence the immune cell composition in mesenteric lymph nodes, shown by an increase in total B cell population and a decrease in total T cell and neutrophil populations [ 54 ]. Studies have demonstrated that A. muciniphila highly correlated with IL-10 in vivo and in vitro; both live and pasteurized A. muciniphila could restore the downregulation of IL-10 in UC mice and in H7N9-infected mice [ 55 , 56 ]. In vitro, A. muciniphila could increase IL-10 release in whole blood ex vivo LPS-stimulated mice as well as Porphyromonas gingivalis -infected bone marrow macrophages (BMMϕ) [ 54 , 57 ].…”

Section: Discussionmentioning

confidence: 99%

Periplaneta americana Extract Pretreatment Alleviates Oxidative Stress and Inflammation and Increases the Abundance of Gut Akkermansia muciniphila in Diquat-Induced Mice

Chen

et al. 2022

Antioxidants

View full text Add to dashboard Cite

Studies have shown that Periplaneta americana extract (PAE) has good therapeutic effects in inflammatory disorders such as ulcerative colitis, alcoholic hepatitis, and gastric ulcers. However, whether or not PAE has good pre-protective effects has not been widely and deeply studied. In this study, we investigated the effects of PAE pretreatment for 7 days on oxidative stress and inflammation triggered by oxidative stress by using diquat-induced C57BL/6 mice as an oxidative stress model. The results showed that PAE pretreatment could significantly reduce oxidative stress in the intestine and liver by reducing the production of MDA, and improved antioxidant systems (SOD, CAT, GSH, and T-AOC). By primarily activating the anti-inflammatory cytokine (IL-10) mediated JAK1/STAT3 signaling pathway, PAE also effectively reduced oxidative stress-induced liver inflammation while also reducing liver damage, as evidenced by the reductions in serum AST and ALT. PAE pretreatment also had a significant effect on maintaining the intestinal barrier function, which was manifested by inhibiting a decrease in the expression of tight junction proteins (ZO-1 and occludin), and reducing the increased intestinal permeability (serum DAO and D-Lac) caused by diquat. The 16S rRNA sequencing analysis revealed that diquat decreased the gut microbiota diversity index and increased the abundance of pathogenic bacteria (e.g. Allobaculum, Providenci,a and Escherichia-Shigella), while PAE pretreatment responded to diquat-induced damage by greatly increasing the abundance of Akkermansia muciniphila. These findings elucidate potential pre-protective mechanisms of PAE in alleviating oxidative stress and inflammation, while providing a direction for the treatment of metabolic diseases by utilizing PAE to enhance the abundance of gut A. muciniphila.

show abstract

Section: Discussionmentioning

confidence: 99%

Periplaneta americana Extract Pretreatment Alleviates Oxidative Stress and Inflammation and Increases the Abundance of Gut Akkermansia muciniphila in Diquat-Induced Mice

Chen

et al. 2022

Antioxidants

View full text Add to dashboard Cite

show abstract

“…In recent years, SCFA released by gut microbiota has become a hot spot in the research of OP because it plays an important role in regulation of osteoclast metabolism and bone homeostasis 44 . Therefore, besides untargeted metabolomics in serum, SCFAs metabolomics analysis in feces was also carried out in this experiment.…”

Section: Discussionmentioning

confidence: 99%

Integrated Metagenomic and Metabolomic Analyses of the Effects of Total Flavonoids of Rhizoma Drynariae on Reducing Ovariectomized-Induced Osteoporosis by Regulating Gut Microbiota and Related Metabolites

Fang

Chu

et al. 2022

Preprint

View full text Add to dashboard Cite

Ethnopharmacological relevance: Total flavonoids of Rhizoma Drynariae (TFRD), an important component extracted from the dried rhizome of Drynaria roosii Nakaike [Polypodiaceae]. TFRD has been widely used in China to treat osteoporosis (OP). However, the specific molecular mechanism of TFRD against OP has not been fully clarified. Aim of the study: The aim of this study was to further elucidate molecular mechanism of TFRD for treating OP based on metagenomic and metabolomic analyses. Materials and methods: In this study, hematoxylin-eosin (H&E) staining, micro computed tomography (micro-CT) and bone mineral density (BMD) analysis were used to observe pharmacological effects of TFRD against ovariectomized (OVX)-induced OP. Subsequently, multiomics analysis including metagenomics, untargeted and short chain fatty acids (SCFAs) metabolomics were carried out to identify whether the anti-osteoporosis mechanism of TFRD correlated with gut microbiota and related metabolites. Results: TFRD could improve the microstructure and density of trabecular bone in OVX rats. 17 differential species, which mainly from Akkermansia, Bacteroides, and Phascolarctobacteriumgenus, 14 related differential metabolites and acetic acid in SCFAs were significantly altered by OVX and reversed by TFRD. Furthermore, according to results of untargeted metabolomics analysis, it was found several metabolic pathways such as phenylalanine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis and so on might play an important role in TFRD against OP. In order to further study the relationship between gut microbiota and related metabolites, spearman correlation analysis was used, and showed that gut microbiota such as Akkermansia muciniphila might be closely related to several metabolites and metabolic pathways. Conclusions: TFRD treatment could reduce the effects of OVX-induced OP by altering community composition and abundance of gut microbiota, regulating metabolites and SCFAs. It was speculated that the gut microbiota especially Akkermansia muciniphila and related metabolites might play an important role in TFRD against OP, and deserve further study by follow-up experiment. This conclusion provides new theoretical support for mechanism research of TFRD against OP.

show abstract

“… 28 , 47 Since the discovery of Amuc 1100, many favorable effects of A. muciniphila on host health have been attributed to the protein. These include anti-inflammatory and anti-tumorigenesis activity in DSS/azoxymethane treated mice, 79 restoration of aberrant tryptophan levels in colitis mice, 80 up-regulation of serotonin (i.e., 5-HT) metabolism in RIN-14B and Caco-2 cells, 81 and improvement of depression-like behavior in chronic stress-induced mice. 82 The beneficial health effects observed with a single, purified, A. muciniphila protein have expanded prospects for the commercialization of A. muciniphila , particularly in light of the difficulty encountered in culturing live, whole bacteria in bulk.…”

Section: A Muciniphila As a Biotherapeutic Agent: The Missin...mentioning

confidence: 99%

Revisiting the role of Akkermansia muciniphila as a therapeutic bacterium

Kang

You

et al. 2022

Gut Microbes

View full text Add to dashboard Cite

Despite a short history since its first isolation, Akkermansia muciniphila has been extensively studied in relation to its effects on human metabolism. A recent human intervention study also demonstrated that the bacterium is safe to use for therapeutic purposes. The best-known effects of A. muciniphila in human health and disease relate to its ability to strengthen gut integrity, modulate insulin resistance, and protect the host from metabolic inflammation. A further molecular mechanism, induction of GLP-1 secretion through ICAM-2 receptor, was recently discovered with the identification of a new bacterial protein produced by A. muciniphila . However, other studies have suggested a detrimental role for A. muciniphila in specific host immune settings. Here, we evaluate the molecular, mechanistic effects of A. muciniphila in host health and suggest some of the missing links to be connected before the organism should be considered as a next-generation biotherapeutic agent.

show abstract

Akkermansia muciniphila and its outer protein Amuc_1100 regulates tryptophan metabolism in colitis

Abstract: Dietary interventions, including dietary ingredients, nutrients and probiotics, exert anti-inflammation effects in ulcerative colitis (UC). Our previous study showed that Akkermansia muciniphila (Akk), a promising probiotics, could protect against colitis...

Cited by 81 publications

References 41 publications

Periplaneta americana Extract Pretreatment Alleviates Oxidative Stress and Inflammation and Increases the Abundance of Gut Akkermansia muciniphila in Diquat-Induced Mice

Periplaneta americana Extract Pretreatment Alleviates Oxidative Stress and Inflammation and Increases the Abundance of Gut Akkermansia muciniphila in Diquat-Induced Mice

Integrated Metagenomic and Metabolomic Analyses of the Effects of Total Flavonoids of Rhizoma Drynariae on Reducing Ovariectomized-Induced Osteoporosis by Regulating Gut Microbiota and Related Metabolites

Revisiting the role of Akkermansia muciniphila as a therapeutic bacterium

Contact Info

Product

Resources

About