<i>Alchemilla vulgaris</i> agg. (Lady's mantle) from central Balkan: antioxidant, anticancer and enzyme inhibition properties

Vlaisavljević, Sanja; Jelača, Sanja; Zengin, Gökhan; Mimica–Dukić, Neda; Berežni, Sanja; Miljić, M; Stevanović, Z. D.

doi:10.1039/c9ra08231j

Cited by 28 publications

(45 citation statements)

References 55 publications

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“…A number of food and medicinal plants have been processed for primary-and secondary-metabolite determination [22] to establish a possible link between their phytocomponents and beneficial bioactivities. It is well known that the polyphenolics and flavonoids, as well as their glycosides, are responsible for antioxidant, anticancer, and cardioprotective activity [23]. The HPLC-PDA analysis results showed that the leaf and stem extracts contained diverse phenolic compounds, with epicatechin, rutin, and catechin as the most abundant.…”

Section: Discussionmentioning

confidence: 99%

“…The HPLC-PDA analysis results showed that the leaf and stem extracts contained diverse phenolic compounds, with epicatechin, rutin, and catechin as the most abundant. Plants contain different classes of phenols, including polyphenols, flavonoids, phenolic acids, stilbenes, and lignins, which are involved in the protection against and cure of diseases such as cancers, cardiovascular diseases, diabetes, and others [23,24]. Flavonoids are the most abundant polyphenol in human diets.…”

Section: Discussionmentioning

confidence: 99%

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Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

Ali

Saleem

Mannan

et al. 2020

BMC Complement Med Ther

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Background Ethnobotanical and plant-based products allow for the isolation of active constituents against a number of maladies. Monotheca buxifolia is used by local communities due to its digestive and laxative properties, as well as its ability to cure liver, kidney, and urinary diseases. There is a need to explore the biological activities and chemical constituents of this medicinal plant. Methods In this work, the biochemical potential of M. buxifolia (Falc.) A. DC was explored and linked with its biological activities. Methanol and chloroform extracts from leaves and stems were investigated for total phenolic and flavonoid contents. Ultrahigh-performance liquid chromatography coupled with mass spectrometry (UHPLC–MS) was used to determine secondary-metabolite composition, while high-performance liquid chromatography coupled with photodiode array detection (HPLC–PDA) was used for polyphenolic quantification. In addition, we carried out in vitro assays to determine antioxidant potential and the enzyme-inhibitory response of M. buxifolia extracts. Results Phenolics (91 mg gallic-acid equivalent (GAE)/g) and flavonoids (48.86 mg quercetin equivalent (QE)/g) exhibited their highest concentration in the methanol extract of stems and the chloroform extract of leaves, respectively. UHPLC–MS analysis identified a number of important phytochemicals, belonging to the flavonoid, phenolic, alkaloid, and terpenoid classes of secondary metabolites. The methanol extract of leaves contained a diosgenin derivative and polygalacin D, while kaempferol and robinin were most abundant in the chloroform extract. The methanol extract of stems contained a greater peak area for diosgenin and kaempferol, whereas this was true for lucidumol A and 3-O-cis-coumaroyl maslinic acid in the chloroform extract. Rutin, epicatechin, and catechin were the main phenolics identified by HPLC–PDA analysis. The methanol extract of stems exhibited significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging activities (145.18 and 279.04 mmol Trolox equivalent (TE)/g, respectively). The maximum cupric reducing antioxidant capacity (CUPRAC) (361.4 mg TE/g), ferric-reducing antioxidant power (FRAP) (247.19 mg TE/g), and total antioxidant potential (2.75 mmol TE/g) were depicted by the methanol extract of stems. The methanol extract of leaves exhibited stronger inhibition against acetylcholinesterase (AChE) and glucosidase, while the chloroform extract of stems was most active against butyrylcholinesterase (BChE) (4.27 mg galantamine equivalent (GALAE)/g). Similarly, the highest tyrosinase (140 mg kojic-acid equivalent (KAE)/g) and amylase (0.67 mmol acarbose equivalent (ACAE)/g) inhibition was observed for the methanol extract of stems. Conclusions UHPLC–MS analysis and HPLC–PDA quantification identified a number of bioactive secondary metabolites of M. buxifolia, which may be responsible for its antioxidant potential and enzyme-inhibitory response. M. buxifolia can be further explored for the isolation of its active components to be used as a drug.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

Ali

Saleem

Mannan

et al. 2020

BMC Complement Med Ther

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“…It might be added that aside from wound or burn healing, collagenase inhibitors might be useful in the treatment of other skin-related diseases, such as dermatitis, acne, urticarial, or photoaging (Mandrone et al, 2018). Also, Vlaisavljević et al (2019) revealed amylase and tyrosinase inhibitory effects of A. vulgaris, which might be related to antidiabetic and skin-related diseases (Vlaisavljević et al, 2019). Improvement of wound healing of lady's mantle was also connected to its anti-inflammatory effects (Ghedira et al, 2012), which were recently confirmed in the study of Boroja et al (2018) where a methanolic extract of aerial part of this plant (in the concentration of 50 µg/mL) was shown to inhibit the activity of COX-1 enzyme by 44.4 %, whereas the inhibition of COX-2 was higher (63.6 %) (Boroja et al, 2018).…”

Section: Wound Healingmentioning

confidence: 99%

“…Such results were ascribed to the high amount of polyphenols in the tested extract, where in the one obtained from Alchemillae herba the major identified compound was ellagic acid (Boroja et al, 2018). Similarly, Vlaisavljević et al (2019) used several antioxidant assays (DPPH, ABTS, CUPRAC, phosphomolybdenum, metal chelating, FRAP) to compare the antioxidant potential of methanolic, ethanolic, ethyl-acetate, and water extracts of lady's mantle. The authors found a strong correlation between total phenolic content and antioxidant activity, with ethyl-acetate extract having the highest content of total phenol compounds (9.65 mg GAE/g) and therefore the highest antioxidant activity.…”

Section: Antioxidant Activitymentioning

confidence: 99%

“…pratensis and subsp. vulgaris) (Vlaisavljević et al, 2019). According to the phytochemical studies, the aerial part of the plant contains mostly phenolic compounds -a large amount of tannins (el-lagitannins, the main one is agrimoniin), phenolcarboxylic acids (ellagic, gallic and caffeic acid) and flavonoids (quercetin and kaempferol, and their glycosides) (Shilova et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Lady's mantle (Alchemilla vulgaris L., Rosaceae): A review of traditional uses, phytochemical profile, and biological properties

Tadić¹,

Krgović²,

Žugić³

2020

Lekovite sirovine

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Lady's mantle (Alchemilla vulgaris L. syn. Alchemilla xanthochlora Rothm., Rosaceae) has been commonly used in folk medicine to heal inflammations in the mouth, bleeding of the nose, furuncules, gynaecological (menorrhagia and dysmenorrhoea), and gastrointestinal disorders. Although therapeutic indications for lady's mantle are non-specific diarrhoea and gastrointestinal complaints, it has been reported to exert, as well, a variety of biological activities, including wound healing, antimicrobial, neuroprotective, gastroprotective, cytotoxic, and antioxidant. Lady's mantle presents a valuable source of natural bioactive compounds, mostly phenolic compounds - a large amount of tannins, phenolcarboxylic acids, and flavonoids, being responsible for the abovementioned effects. In this work, a literature review of biological properties, investigated in in vitro and in vivo experiments in regard to the determined chemical profile is presented. In addition, the data reported are discussed, and the directions for further investigations are proposed.

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Phytochemical Analysis and Assessment of Antioxidant and Enzyme Inhibitory Activity of Alchemilla pseudocartalinica Juz.

Uzunkaya,

Gökkaya,

Akkaya

et al. 2024

Chemistry & Biodiversity

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This study aimed to evaluate and compare the antioxidant capacity and enzyme inhibitory activity of extracts, sub‐extracts, and fractions prepared from the aerial parts and roots of A. pseudocartalinica. The phytochemical content of the active extracts was also analyzed. According to the results, ellagic acid (38.42 mg/g) was the major compound in the aerial part methanol extract and catechin (185.30 mg/g) in the root methanol extract. The DPPH inhibition activity of all fractions was monitored, with the most active one (Fr B) reaching an IC50 value of (4.92 ± 0.59 µg/mL). All the fractions prepared from the aerial parts' water sub‐extract showed higher a‐glucosidase inhibitory activity than the positive control acarbose. In the tyrosinase assay, Fr B (58.81 ± 7.50 µg/mL) exhibited the highest inhibitory actions among all fractions. The structure of the major substances of the most active fraction were elucidated as quercetin 7‐O‐β‐glucopyranosyl‐3‐O‐β‐glucuronopyranoside‐ (1), and α‐[(2‐formyl‐5‐hydroxymethyl)pyrrol‐1‐yl]aspartic acid (2).

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Alchemilla vulgaris agg. (Lady's mantle) from central Balkan: antioxidant, anticancer and enzyme inhibition properties

Abstract: The current study was designed to evaluate the phytochemical profile and biological properties of extracts of Alchemilla vulgaris from Southeast Serbia which was traditionally used to alleviate and treat many diseases.

Cited by 28 publications

References 55 publications

Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

Lady's mantle (Alchemilla vulgaris L., Rosaceae): A review of traditional uses, phytochemical profile, and biological properties

Phytochemical Analysis and Assessment of Antioxidant and Enzyme Inhibitory Activity of Alchemilla pseudocartalinica Juz.

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