<i>AMF-SporeChip</i> provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

Richter, Felix; Calonne-Salmon, Maryline; van der Heijden, Marcel G. A.; Declerck, Stéphane; Stanley, Claire E.

doi:10.1039/d3lc00859b

Cited by 2 publications

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“…2018). For AMF spores, Richter et al have recently presented the AMF-SporeChip , which revealed new insights into AMF spore germination and hyphal growth (Richter et al . 2024).…”

Section: Introductionmentioning

confidence: 99%

“…The microfluidic single-cell cultivation (MSCC) device employs arrays of microchambers, allowing analysis of the germination of the filamentous bacterium Streptomyces lividans under multiple media compositions, which revealed surprising stability in behaviour despite previous observations in large-scale cultures (Koepff et al 2018). For AMF spores, Richter et al have recently presented the AMF-SporeChip, which revealed new insights into AMF spore germination and hyphal growth (Richter et al 2024).…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic platform for microbial spore germination studies in multiple growth conditions

Bernier,

Estoppey,

Bindschedler

et al. 2024

Preprint

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Spores are highly resistant dormant cells, adapted for survival and dispersal, that can withstand unfavourable environmental conditions for extended periods of time and later reactivate. Understanding the germination process of microbial spores is important in numerous areas including agriculture, food safety and health, and other sectors of biotechnology. Microfluidics combined with high-resolution microscopy allows to study spore germination at the single-cell level, revealing behaviours that would be hidden in standard population-level studies. Here, we present a microfluidic platform for germination studies where spores are confined to monolayers inside microchambers, allowing the testing of four growth conditions in parallel. This platform can be used with multiple species, including non-model organisms, and is compatible with existing image analysis software. In this study, we focused on three soil dwellers, two prokaryotes and one fungus, and revealed new insights into their germination. We studied endospores of the model bacterium Bacillus subtilis and demonstrated a correlation between spore density and germination in rich media. We then investigated the germination of the obligate-oxalotrophic environmental bacterium Ammoniphilus oxalaticus in a concentration gradient of potassium oxalate, showing that lower concentrations result in more spores germinating compared to higher concentrations. We also used this microfluidic platform to study the soil beneficial filamentous fungus Trichoderma rossicum, showing for the first time that the size of the spores and hyphae increase in response to increased nutrient availability, while germination times remain the same. Our platform allows to better understand microbial behaviour at the single-cell level, under a variety of controlled conditions.

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“…2018). For AMF spores, Richter et al have recently presented the AMF-SporeChip , which revealed new insights into AMF spore germination and hyphal growth (Richter et al . 2024).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microfluidic platform for microbial spore germination studies in multiple growth conditions

Bernier,

Estoppey,

Bindschedler

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Microfluidic platform for microbial spore germination studies in multiple growth conditions

Bernier,

Estoppey,

Bindschedler

et al. 2024

BMC Methods

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AMF-SporeChip provides new insights into arbuscular mycorrhizal fungal asymbiotic hyphal growth dynamics at the cellular level

Abstract: Arbuscular mycorrhizal fungi (AMF) form symbiotic associations with the majority of land plants and deliver a wide range of soil-based ecosystem services. Due to their conspicuous belowground lifestyle in a...

Cited by 2 publications

References 85 publications

Microfluidic platform for microbial spore germination studies in multiple growth conditions

Microfluidic platform for microbial spore germination studies in multiple growth conditions

Microfluidic platform for microbial spore germination studies in multiple growth conditions

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