Ancrocorticia populi gen. nov., sp. nov, isolated from the symptomatic bark of Populus × euramericana canker

Abstract

A Gram‐staining positive facultative anaerobic, non‐motile strain, sk1b4 T , was isolated from canker of symptomatic bark tissue of a Populus × euramericana . 16S rRNA gene sequence analyses showed that strain sk1b4 T shared the highest similarity with Arcanobacterium phocisimile (94.1%). Within the phylogenetic tree, the novel isolate formed a distinct branch from Actinobaculum , Arcanobacterium , and Trueperella . The percentage of conserved proteins calculated from genomic sequence indicated a low level of relatedness between the novel strain and its phylogenetic neighbors. Growth of the novel strain occurred at temperatures between 10 and 41°C, and within a pH range of 6.0–9.0; optimal growth occurred at 30°C and at pH 6.0–9.0. Growth also occurred within a NaCl concentration of 1%–5% (w/v). The major fatty acids of the strain were C 14:0 , C 16:0 , and C 18:1 ω 9 c , and major polar lipids were glycolipid, phosphatidylinositol mannoside, phospholipid, diphosphatidylglycerol, and phosphatidylglycerol. Respiratory quinone was absent. On the basis of phenotypic and genotypic characteristics, we propose that the novel isolate should be classified as a novel species in a new genus: Ancrocorticia populi gen. nov., sp. nov. The type strain is sk1b4 T (=CFCC 14564 T = KCTC 39919 T ).

Metagenomic and Culturomics Analysis of Microbial Communities within Surface Sediments and the Prevalence of Antibiotic Resistance Genes in a Pristine River: The Zaqu River in the Lancang River Source Region, China

“…Compared with their type strains, four potential novel species with 16S rRNA gene sequence shared a similarity of less than 98.65%. The type strains of the four potential novel species in this study were Ancrocorticia populi sk1b4 (T) [104], Jeotgalibaca porci CCUG 69148 (T) [105], Brooklawnia cerclage BL-34 (T) [103], and Leucobacter humi Re6 (T) [102]. Further investigations will delve into their morphological, phenotypic, and chemotaxonomic characteristics.…”