Multidisciplinary studies integrating, U-Pb geochronology, whole-rock geochemical data, isotope geochemistry, anisotropy of magnetic susceptibility (AMS) studies and gravimetry were carried out on the Vila Pouca de Aguiar and the Águas Frias-Chaves porphyritic biotite granite plutons. Both plutons occur independently in a distance of about 20 km. The Vila Pouca de Aguiar and Águas Frias-Chaves plutons are examples of late to post-orogenic felsic Variscan granites in northern Portugal (NW Iberian Peninsula). The U-Pb zircon analyses yield a consistent age of 299 ± 3 Ma which is considered to be the emplacement age of the two plutons. These granites are weakly peraluminous, show high HREE and Y (and low P) contents which are consistent with them being I-type. This is also supported by their weakly evolved isotopic compositions, 87 Sr/ 86 Sr i = 0.7044-0.7077 and εNd = − 2.0 to − 2.6, as well as by the whole rock oxygen isotope (δ 18 O VSMOW) ranging from + 9.7‰ to + 11.0‰. The emplacement of granite magma took place after the third Variscan deformation phase (D 3 ) in an extensional tectonic regime, large scale uplift and crustal thinning. The integration of different data suggests that both plutons have the same feeding zone aligned within the Penacova-Régua-Verin fault (PRVF) and that both have the same structure which is related to late Variscan phases. The thicker shape for the Águas Frias-Chaves pluton comparing to that of the Vila Pouca de Aguiar pluton is compatible with different depths of PRVF sectors. The available data led us to propose a model of partial melting of a meta-igneous lower crustal source rather than an open-system of mantle-crust interaction. The interaction between the continental crust and invading mafic magmas could have been limited to mere heat transfer and, perhaps, local intermingling.