Anopheles gambiae: Metabolomic Profiles in Sugar‐Fed, Blood‐Fed, and Plasmodium falciparum‐Infected Midgut

Champion, Cody; Kukutla, Phanidhar; Glennon, Elizabeth K.K.; Wang, Bo; Luckhart, Shirley; Xu, Jianchu

doi:10.1155/2017/8091749

Cited by 11 publications

(8 citation statements)

References 19 publications

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“…Most of these metabolites (79%: 38/48), notably amino acids, were enriched after blood-feeding. Our results are consistent with precedent studies that have compared metabolomic profiles of sugar-fed and blood-fed Anopheles gambiae [ 22 , 23 ].…”

Section: Resultssupporting

confidence: 92%

Antibiotic Treatment in Anopheles coluzzii Affects Carbon and Nitrogen Metabolism

et al. 2020

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The mosquito microbiota reduces the vector competence of Anopheles to Plasmodium and affects host fitness; it is therefore considered as a potential target to reduce malaria transmission. While immune induction, secretion of antimicrobials and metabolic competition are three typical mechanisms of microbiota-mediated protection against invasive pathogens in mammals, the involvement of metabolic competition or mutualism in mosquito-microbiota and microbiota-Plasmodium interactions has not been investigated. Here, we describe a metabolome analysis of the midgut of Anopheles coluzzii provided with a sugar-meal or a non-infectious blood-meal, under conventional or antibiotic-treated conditions. We observed that the antibiotic treatment affects the tricarboxylic acid cycle and nitrogen metabolism, notably resulting in decreased abundance of free amino acids. Linking our results with published data, we identified pathways which may participate in microbiota-Plasmodium interactions via metabolic interactions or immune modulation and thus would be interesting candidates for future functional studies.

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Section: Resultssupporting

confidence: 92%

Antibiotic Treatment in Anopheles coluzzii Affects Carbon and Nitrogen Metabolism

et al. 2020

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“…Most of these metabolites (79% -38/48), notably amino acids, were enriched after blood-feeding. Our results are consistent with precedent studies that have compared metabolomic profiles of sugar-fed and blood-fed Anopheles gambiae [21,22]. The y axis represents the negative log10 applied to p-values, metabolites located above the green line are significantly affected (p<0.05, paired t-test).…”

Section: Resultssupporting

confidence: 90%

Antibiotic Treatment in An. coluzzii Affects Carbon and Nitrogen Metabolism

Chabanol¹,

Behrends²,

Prévot³

et al. 2020

Preprint

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The mosquito microbiota reduces the vector competence of Anopheles to Plasmodium and affects host fitness, it is therefore considered as a potential target to reduce malaria transmission. While immune induction, secretion of antimicrobials and metabolic competition are three typical mechanisms of microbiota-mediated protection against invasive pathogens in mammals, the involvement of metabolic competition or mutualism in mosquito-microbiota and microbiota-Plasmodium interactions has not been investigated. Here, we describe a metabolome analysis of the midgut of An. coluzzii provided with a sugar-meal or a blood-meal, under conventional or antibiotic-treated conditions. We observed that the antibiotic treatment affects the tricarboxylic acid cycle and nitrogen metabolism, notably resulting in decreased abundance of free amino acids. Linking our results with published data, we identified candidate pathways which may participate in microbiota/Plasmodium interactions via metabolic interactions or immune modulation.

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“…Metabolomics analysis of An. gambiae 24 h pbf by Champion & Xu [146], revealed an increased concentration of glucose 6 phosphate and 6-phosphogluconate. This may be indicative of increased expression of the enzymes that are involved in their production, namely, glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6-PGDH).…”

Section: Metabolic Proteins and Blood Digestion In Anophelesmentioning

confidence: 97%

Anopheles metabolic proteins in malaria transmission, prevention and control: a review

et al. 2020

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The increasing resistance to currently available insecticides in the malaria vector, Anopheles mosquitoes, hampers their use as an effective vector control strategy for the prevention of malaria transmission. Therefore, there is need for new insecticides and/or alternative vector control strategies, the development of which relies on the identification of possible targets in Anopheles. Some known and promising targets for the prevention or control of malaria transmission exist among Anopheles metabolic proteins. This review aims to elucidate the current and potential contribution of Anopheles metabolic proteins to malaria transmission and control. Highlighted are the roles of metabolic proteins as insecticide targets, in blood digestion and immune response as well as their contribution to insecticide resistance and Plasmodium parasite development. Furthermore, strategies by which these metabolic proteins can be utilized for vector control are described. Inhibitors of Anopheles metabolic proteins that are designed based on target specificity can yield insecticides with no significant toxicity to non-target species. These metabolic modulators combined with each other or with synergists, sterilants, and transmission-blocking agents in a single product, can yield potent malaria intervention strategies. These combinations can provide multiple means of controlling the vector. Also, they can help to slow down the development of insecticide resistance. Moreover, some metabolic proteins can be modulated for mosquito population replacement or suppression strategies, which will significantly help to curb malaria transmission.

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Anopheles gambiae: Metabolomic Profiles in Sugar‐Fed, Blood‐Fed, and Plasmodium falciparum‐Infected Midgut

Cited by 11 publications

References 19 publications

Antibiotic Treatment in Anopheles coluzzii Affects Carbon and Nitrogen Metabolism

Antibiotic Treatment in Anopheles coluzzii Affects Carbon and Nitrogen Metabolism

Antibiotic Treatment in <em>An. coluzzii</em> Affects Carbon and Nitrogen Metabolism

Anopheles metabolic proteins in malaria transmission, prevention and control: a review

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