AnophelesEgg Collection, Disinfection, and Hatching

Abstract

Gravid (i.e., with fully developed eggs), matedAnophelesfemales typically lay their eggs directly on water ∼48–72 h after a blood meal. Unlike some other mosquito species,Anopheleseggs cannot be desiccated and stored for long durations, and, hence, colonies must be reared continuously. In this protocol, we discuss methods for egg collection, including individual anden masseoviposition; egg disinfection to avoid the transmission of infectious agents to the next generation; and egg hatching for colony maintenance or experimentation. We also include optional methods for estimating life history traits such as fecundity, fertility, and larval mortality rates from egg counts.