Antheraea pernyi silk fibroin-coated adenovirus as a VEGF165-Ang-1 dual gene delivery vector

Abstract

Vascularization is a key challenge in the regeneration of tissues containing blood vessels. In this study, spermine was used for cationic modification of Antheraea pernyi silk fibroin (ASF) to synthesize cationized ASF (CASF). CASF/Ad complexes prepared by coating adenovirus (Ad) with CASF were used as delivery vectors for vascular endothelial growth factor 165 and angiopoietin-1 dual genes. The results showed that the zeta potential of the Ad was reversed from −7.75 mV to approximately +8.40 mV after CASF coating, and the sizes of the CASF/Ad complexes were 200 to 290 nm. Furthermore, human umbilical vein endothelial cells HUVECs were cocultured and infected with CASF/Ad in vitro. The results of confocal laser scanning microscopy, flow cytometry and CCK-8 assay showed that coating Ad with CASF at concentration of 20 and 50 µg/mL not only reduced the cytotoxicity of naked Ad, but also significantly promoted cell proliferation. Therefore, the CASF/Ad complexes could be beneficial to reduce the dosage of Ad and the potential toxicity risk of high doses of Ad in vivo, which has the potential of application to promote vascular network regeneration.