<i>AOX</i>
            diversity studies stimulate novel tool development for phenotyping

Arnholdt‐Schmitt, Birgit; Hansen, Lee D.; Nogales, Amaia; Muñoz-Sanhueza, Luz

doi:10.1002/9781118789971.ch19

Cited by 2 publications

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“…However, despite this early insight, the concept has never been applied on important crops in global breeding or for farmers in order to select the better material for their region. More recently, the basic concept of this approach got significant support through new knowledge on the significance of mitochondria and specifically on alternative respiration for managing plants' adaptive multi-stress responses (reviewed in [3] and [4]) combined with deep phenotyping initiatives in plant breeding [5][6][7]. Alternative oxidase (AOX) genes have been proposed as promising candidates for functional marker development linked to temperature responses and growth performance [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Respiration Traits as Novel Markers for Plant Robustness Under the Threat of Climate Change: A Protocol for Validation

Arnholdt‐Schmitt

2017

Methods in Molecular Biology

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Respiration traits allow calculating temperature-dependent carbon use efficiency and prediction of growth rates. This protocol aims (1) to enable validation of respiration traits as non-DNA biomarkers for breeding on robust plants in support of sustainable and healthy plant production; (2) to provide an efficient, novel way to identify and predict functionality of DNA-based markers (genes, polymorphisms, edited genes, transgenes, genomes, and hologenomes), and (3) to directly help farmers select robust material appropriate for a specified region. The protocol is based on applying isothermal calorespirometry and consists of four steps: plant tissue preparation, calorespirometry measurements, data processing, and final validation through massive field-based data.The methodology can serve selection and improvement for a wide range of crops. Several of them are currently being tested in the author's lab. Among them are important cereals, such as wheat, barley, and rye, and diverse vegetables. However, it is critical that the protocol for measuring respiration traits be well adjusted to the plant species by considering deep knowledge on the specific physiology and functional cell biology behind the final target trait for production. Here, Daucus carota L. is chosen as an advanced example to demonstrate critical species-specific steps for protocol development. Carrot is an important global vegetable that is grown worldwide and in all climate regions (moderate, subtropical, and tropical). Recently, this species is also used in my lab as a model for studies on alternative oxidase (AOX) gene diversity and evolutionary dynamics in interaction with endophytes.

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Section: Introductionmentioning

confidence: 99%

Respiration Traits as Novel Markers for Plant Robustness Under the Threat of Climate Change: A Protocol for Validation

Arnholdt‐Schmitt

2017

Methods in Molecular Biology

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Phenotyping carrot (Daucus carota L.) for yield-determining temperature response by calorespirometry

Nogales

Muñoz-Sanhueza

Hansen

et al. 2014

Planta

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Calorespirometric measurements proved to be useful for phenotyping temperature response in terms of optimum temperatures for growth and low temperature limits for growth respiration in diverse carrot genotypes. High and low-temperature tolerance is an important trait in many breeding programs, but to date, improvement strategies have had limited success. Developing new, cost efficient and reliable screening tools to identify and select the most tolerant crop plant genotypes is necessary to assist plant breeding on cold and heat tolerance, and calorespirometry is proposed for this. Calorespirometry is a technique to simultaneously measure metabolic heat rates and CO2 emission rates of respiring tissues and can be used as a rapid method to determine how changes in the environment (e.g., temperature) influence plant growth. The main aim of this work was, therefore, to test the usefulness of calorespirometry as a phenotyping tool for carrot taproot growth in response to temperature. Calorespirometric measurements in the carrot taproot meristems of plants from eight carrot inbred lines allowed identification of optimum and minimum temperatures for growth of plants and to distinguish between phenotypes based on those characteristics. The technique proved to be useful for predicting yield-determining temperature responses in diverse carrot genotypes. Preliminary screening of new crop plant genotypes with calorespirometry based on their temperature adaptation and acclimation capability could make the screening process much less laborious by allowing selection of genotypes presenting the best growth performance under particular biotic or abiotic conditions before field tests.

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AOX diversity studies stimulate novel tool development for phenotyping

Cited by 2 publications

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Respiration Traits as Novel Markers for Plant Robustness Under the Threat of Climate Change: A Protocol for Validation

Respiration Traits as Novel Markers for Plant Robustness Under the Threat of Climate Change: A Protocol for Validation

Phenotyping carrot (Daucus carota L.) for yield-determining temperature response by calorespirometry

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