2022
DOI: 10.1073/pnas.2208415119
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Arabidopsis AAR2, a conserved splicing factor in eukaryotes, acts in microRNA biogenesis

Abstract: MicroRNAs (miRNAs) play an essential role in plant growth and development, and as such, their biogenesis is fine-tuned via regulation of the core microprocessor components. Here, we report that Arabidopsis AAR2, a homolog of a U5 snRNP assembly factor in yeast and humans, not only acts in splicing but also promotes miRNA biogenesis. AAR2 interacts with the microprocessor component hyponastic leaves 1 (HYL1) in the cytoplasm, nucleus, and dicing bodies. In aar2 mu… Show more

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Cited by 13 publications
(15 citation statements)
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“…Several miRNA biogenesis factors have been reported to be involved in D‐body formation. Mutants of MOS2 (Wu et al., 2013), PRL1 (Zhang et al., 2014), CDC5 (Zhang et al., 2013), MAC7 (Jia et al., 2017), PP4R3 (Wang, Chen, et al., 2019; Wang, Quan, et al., 2019), THP1 (Zhang et al., 2020), RBV (Liang et al., 2022), AAR2 (Fan et al., 2022), and SEAP1 (Li, Liu, et al., 2021; Li, Yu, et al., 2021) exhibit a reduced number of D‐bodies and show reduced levels of miRNAs. Similarly, D‐body number, and miRNA accumulation concomitantly decrease in the rcf1‐4 mutant.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Several miRNA biogenesis factors have been reported to be involved in D‐body formation. Mutants of MOS2 (Wu et al., 2013), PRL1 (Zhang et al., 2014), CDC5 (Zhang et al., 2013), MAC7 (Jia et al., 2017), PP4R3 (Wang, Chen, et al., 2019; Wang, Quan, et al., 2019), THP1 (Zhang et al., 2020), RBV (Liang et al., 2022), AAR2 (Fan et al., 2022), and SEAP1 (Li, Liu, et al., 2021; Li, Yu, et al., 2021) exhibit a reduced number of D‐bodies and show reduced levels of miRNAs. Similarly, D‐body number, and miRNA accumulation concomitantly decrease in the rcf1‐4 mutant.…”
Section: Discussionmentioning
confidence: 99%
“…Nuclear–cytoplasmic fractionation was performed as described (Fan et al., 2022). Twelve‐day‐old seedlings were collected and ground to a fine powder in liquid nitrogen in liquid nitrogen.…”
Section: Methodsmentioning
confidence: 99%
“…Interestingly, AAR2 is involved in HYL1 degradation in the cytoplasm and HYL1 dephosphorylation. In aar2 mutant plants, the pri-microRNA level is reduced although the nonphosphorylated form of HYL1 (active in miRNA biogenesis) level is also reduced [ 100 ]. Splicing of the barley transcripts containing intronic pri-miR160a and pri-miRNA5175a was induced by heat and correlated with the accumulation of mature miRNAs, suggesting the post-transcriptional regulation of miRNA precursor processing [ 101 ].…”
Section: Splicingmentioning
confidence: 99%
“…Interestingly, AAR2 is involved in HYL1 degradation in the cytoplasm and HYL1 dephosphorylation. In aar2 mutant plants, the pri-microRNA level is reduced although the nonphosphorylated form of HYL1 (active in miRNA biogenesis) level is also reduced [ 100 ].…”
Section: Splicingmentioning
confidence: 99%
“…However, C-Terminal Domain Phosphatase-like 1 (CPL1), PP4 and Suppressor of MEK 1 (SMEK1) play roles in the dephosphorylation of HYL1 protein and promote miRNA biogenesis ( Manavella et al., 2012 ; Su et al., 2017 ). AAR2, a homolog of U5 snRNP in yeast and humans, plays a role in splicing and miRNA biogenesis in plants, and promotes HYL1 dephosphorylation to produce the active form HYL1 ( Fan et al., 2022 ). So far, all the available evidence suggest that the non-phosphorylated version of HYL1 as the active form ( Mencia et al., 2022 ).…”
Section: Key Roles Of Microprocessors In the Biosynthesis Of Mirnasmentioning
confidence: 99%