<i>Arabidopsis</i> bZIP16 Transcription Factor Integrates Light and Hormone Signaling Pathways to Regulate Early Seedling Development

Hsieh, Wu‐Chiao; Hsieh, Hsun-Ping; Wu, Shu-Hsing

doi:10.1105/tpc.112.105478

Cited by 66 publications

(44 citation statements)

References 88 publications

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“…Besides regulating the expression of photosynthetic genes, light controls photomorphogenesis and inhibits hypocotyl elongation. Although interactions between different hormones and light signaling have been studied extensively (Chen et al, 2008;Seo et al, 2009;Lau and Deng, 2010;Hsieh et al, 2012), our understanding of the underlying complex regulation is still far from complete. Visual inspection of young seedlings indicated that, in the presence of estradiol, ZFP3ox plants produced shorter hypocotyls than wild-type plants, both in the dark and in white light ( Fig.…”

Section: Zfp3 Modulates Light Responses In Seedling Developmentmentioning

confidence: 99%

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

et al. 2014

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Seed germination is controlled by environmental signals, including light and endogenous phytohormones. Abscisic acid (ABA) inhibits, whereas gibberellin promotes, germination and early seedling development, respectively. Here, we report that ZFP3, a nuclear C2H2 zinc finger protein, acts as a negative regulator of ABA suppression of seed germination in Arabidopsis (Arabidopsis thaliana). Accordingly, regulated overexpression of ZFP3 and the closely related ZFP1, ZFP4, ZFP6, and ZFP7 zinc finger factors confers ABA insensitivity to seed germination, while the zfp3 zfp4 double mutant displays enhanced ABA susceptibility. Reduced expression of several ABA-induced genes, such as RESPONSIVE TO ABSCISIC ACID18 and transcription factor ABSCISIC ACID-INSENSITIVE4 (ABI4), in ZFP3 overexpression seedlings suggests that ZFP3 negatively regulates ABA signaling. Analysis of ZFP3 overexpression plants revealed multiple phenotypic alterations, such as semidwarf growth habit, defects in fertility, and enhanced sensitivity of hypocotyl elongation to red but not to far-red or blue light. Analysis of genetic interactions with phytochrome and abi mutants indicates that ZFP3 enhances red light signaling by photoreceptors other than phytochrome A and additively increases ABA insensitivity conferred by the abi2, abi4, and abi5 mutations. These data support the conclusion that ZFP3 and the related ZFP subfamily of zinc finger factors regulate light and ABA responses during germination and early seedling development.

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Section: Zfp3 Modulates Light Responses In Seedling Developmentmentioning

confidence: 99%

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

et al. 2014

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“…Importantly, although ChIP-X reveals the binding of a TF onto a promoter, it does not indicate whether this results in activation/repression of gene expression [54]. Therefore, ChIP-X has often been combined with genome-wide transcriptional analysis to characterize the primary targets of a TF [55-57]. …”

Section: Validations Of Inferred Grns (An Experimentalist's Point Of mentioning

confidence: 99%

Gene regulatory networks in plants: learning causality from time and perturbation

et al. 2013

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“…The response to ABA is mediated by promoter motifs based on the ACGT core called ABREs (abscisic acid responsive elements) and including the G-box element (CACGTG). This element has been found in previously identified cases of bidirectional promoters which regulate genes involved in ABA response and seed development [19-21], as well as in promoters of genes regulated by light [22,23]. The identity of the nucleotides flanking the ACGT has been found to be an important determinant of the element’s specificity [24,25].…”

Section: Introductionmentioning

confidence: 97%

Bidirectional promoters in seed development and related hormone/stress responses

Kourmpetli

Lee

Hemsley³

et al. 2013

BMC Plant Biol

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BackgroundBidirectional promoters are common in genomes but under-studied experimentally, particularly in plants. We describe a targeted identification and selection of a subset of putative bidirectional promoters to identify genes involved in seed development and to investigate possible coordinated responses of gene pairs to conditions important in seed maturation such as desiccation and ABA-regulation.ResultsWe combined a search for 100–600 bp intergenic regions in the Arabidopsis genome with a cis-element based selection for those containing multiple copies of the G-box motif, CACGTG. One of the putative bidirectional promoters identified also contained a CE3 coupling element 5 bp downstream of one G-box and is identical to that characterized previously in the HVA1 promoter of barley. CE3 elements are significantly under-represented and under-studied in Arabidopsis. We further characterized the pair of genes associated with this promoter and uncovered roles for two small, previously uncharacterized, plant-specific proteins in Arabidopsis seed development and stress responses.ConclusionsUsing bioinformatics we identified putative bidirectional promoters involved in seed development and analysed expression patterns for a pair of plant-specific genes in various tissues and in response to hormones/stress. We also present preliminary functional analysis of these genes that is suggestive of roles in seed development.

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Arabidopsis bZIP16 Transcription Factor Integrates Light and Hormone Signaling Pathways to Regulate Early Seedling Development

Cited by 66 publications

References 88 publications

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

Gene regulatory networks in plants: learning causality from time and perturbation

Bidirectional promoters in seed development and related hormone/stress responses

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