<i>Arabidopsis</i>CBF1 and CBF3 have a different function than CBF2 in cold acclimation and define different gene classes in the CBF regulon

Novillo, Fernando; Medina, Joaquı́n; Salinas, Julio

doi:10.1073/pnas.0705639105

Cited by 328 publications

(289 citation statements)

References 33 publications

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“…They are in the Ws background. CBF loss-of-function lines were obtained from Julio Salinas and have been described previously (Novillo et al, 2007;Col background). dog1-2 (Col; Bentsink et al, 2006) and rdo4 mutants (Ler) were a gift from Marten Koornneef and Wim Soppe, and the gai-t6 rga-t2 rgl2-1 rgl1-1 quadruple DELLA loss-of-function lines were obtained from Nicholas Harberd (Ler background; Achard et al, 2006).…”

Section: Plant Materialsmentioning

confidence: 99%

Induction of Dormancy in Arabidopsis Summer Annuals Requires Parallel Regulation of DOG1 and Hormone Metabolism by Low Temperature and CBF Transcription Factors

et al. 2011

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Summer annuals overwinter as seeds in the soil seed bank. This is facilitated by a cold-induced increase in dormancy during seed maturation followed by a switch to a state during seed imbibition in which cold instead promotes germination. Here, we show that the seed maturation transcriptome in Arabidopsis thaliana is highly temperature sensitive and reveal that low temperature during seed maturation induces several genes associated with dormancy, including DELAY OF GERMINATION1 (DOG1), and influences gibberellin and abscisic acid levels in mature seeds. Mutants lacking DOG1, or with altered gibberellin or abscisic acid synthesis or signaling, in turn show reduced ability to enter the deeply dormant states in response to low seed maturation temperatures. In addition, we find that DOG1 promotes gibberellin catabolism during maturation. We show that C-REPEAT BINDING FACTORS (CBFs) are necessary for regulation of dormancy and of GA2OX6 and DOG1 expression caused by low temperatures. However, the temperature sensitivity of CBF transcription is markedly reduced in seeds and is absent in imbibed seeds. Our data demonstrate that inhibition of CBF expression is likely a critical feature allowing cold to promote rather than inhibit germination and support a model in which CBFs act in parallel to a low-temperature signaling pathway in the regulation of dormancy.

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Section: Plant Materialsmentioning

confidence: 99%

Induction of Dormancy in Arabidopsis Summer Annuals Requires Parallel Regulation of DOG1 and Hormone Metabolism by Low Temperature and CBF Transcription Factors

et al. 2011

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“…The gene encoding C-REPEAT/DRE BINDING FACTOR 1 (CBF1), a regulator of the cold acclimation response (28), and its target genes COLD-REGULATED 47 (COR47) and LOW-TEMPERATURE-INDUCED 78 (LTI78) (29), were up-regulated in the hybrids and most hybrid mimic lines but down-regulated or Table S3). Red/ green represents up-/down-regulated genes in the F1 hybrids.…”

Section: Genes and Metabolic Pathways Important In The F1 Hybrid Phenmentioning

confidence: 99%

Hybrid mimics and hybrid vigor in Arabidopsis

Wang

Greaves

Groszmann

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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F1 hybrids can outperform their parents in yield and vegetative biomass, features of hybrid vigor that form the basis of the hybrid seed industry. The yield advantage of the F1 is lost in the F2 and subsequent generations. In Arabidopsis, from F2 plants that have a F1-like phenotype, we have by recurrent selection produced pure breeding F5/F6 lines, hybrid mimics, in which the characteristics of the F1 hybrid are stabilized. These hybrid mimic lines, like the F1 hybrid, have larger leaves than the parent plant, and the leaves have increased photosynthetic cell numbers, and in some lines, increased size of cells, suggesting an increased supply of photosynthate. A comparison of the differentially expressed genes in the F1 hybrid with those of eight hybrid mimic lines identified metabolic pathways altered in both; these pathways include down-regulation of defense response pathways and altered abiotic response pathways. F6 hybrid mimic lines are mostly homozygous at each locus in the genome and yet retain the large F1-like phenotype. Many alleles in the F6 plants, when they are homozygous, have expression levels different to the level in the parent. We consider this altered expression to be a consequence of transregulation of genes from one parent by genes from the other parent. Transregulation could also arise from epigenetic modifications in the F1. The pure breeding hybrid mimics have been valuable in probing the mechanisms of hybrid vigor and may also prove to be useful hybrid vigor equivalents in agriculture.I n Arabidopsis some ecotypes with similar genome sequences produce F1 hybrids with large increases in vegetative and reproductive yields (1, 2). These results appear to be at variance with the generalization that the larger the genetic distance between parents, the greater the hybrid vigor (3); however, the Arabidopsis ecotypes have different epigenomes that may be important for hybrid vigor (4). In hybrids between C24 and Ler, we found altered levels in two epigenetic systems: 24nt siRNAs and DNA methylation (4, 5). These epigenetic changes appear common among hybrid systems with similar observations being made in maize and rice hybrids (6). The epigenetic changes can correlate with changes in gene expression and contribute to the unique gene expression profile of the F1 hybrid (7). Not all crosses result in hybrid vigor (heterosis); some result in decreased vigor and yield referred to as "hybrid weakness" (8).Heterotic F1 hybrids are featured in agricultural and horticultural crops, and in all species, the yield gains are restricted to the F1 generation. The F2 and subsequent selfed generations are discarded because of reduced yields and heterogeneity of morphological and developmental traits. A hybrid crop system requires an efficient method of F1 hybrid seed production dependent on male sterility in the female parent and synchronous flowering of the male and female parents.QTL analysis in maize and rice has confirmed that hundreds of genome segments contribute to the heterotic phenotype, but the main m...

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“…Novillo et al (2007) reported that CBF1 and CBF3 function additively in cold acclimation and differently from CBF2; CBF2 defines different gene classes in the CBF regulon. Doherty et al (2009) showed that members of calmodulin binding proteins of the CAMTA family of transcription factors can bind to one of the cis-elements in the CBF2 promoter in vitro and are important for freezing tolerance.…”

Section: Introductionmentioning

confidence: 99%

A DEAD Box RNA Helicase Is Critical for Pre-mRNA Splicing, Cold-Responsive Gene Regulation, and Cold Tolerance inArabidopsis

et al. 2013

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Cold stress resulting from chilling and freezing temperatures substantially reduces crop production worldwide. To identify genes critical for cold tolerance in plants, we screened Arabidopsis thaliana mutants for deregulated expression of a firefly luciferase reporter gene under the control of the C-REPEAT BINDING FACTOR2 (CBF2) promoter (CBF2:LUC). A regulator of CBF gene expression1 (rcf1-1) mutant that is hypersensitive to cold stress was chosen for in-depth characterization. RCF1 encodes a cold-inducible DEAD (Asp-Glu-Ala-Asp) box RNA helicase. Unlike a previously reported DEAD box RNA helicase (LOW EXPRESSION OF OSMOTICALLY RESPONSIVE GENES4 [LOS4]) that regulates mRNA export, RCF1 does not play a role in mRNA export. Instead, RCF1 functions to maintain proper splicing of pre-mRNAs; many cold-responsive genes are misspliced in rcf1-1 mutant plants under cold stress. Functional characterization of four genes (PSEUDO-RESPONSE, and SPFH/PHB DOMAIN-CONTAINING MEMBRANE-ASSOCIATED PROTEIN [SPFH]) that are misspliced in rcf1-1 revealed that these genes are cold-inducible positive (CIR1 and SPFH) and negative (PRR5 and SK12) regulators of cold-responsive genes and cold tolerance. Together, our results suggest that the cold-inducible RNA helicase RCF1 is essential for pre-mRNA splicing and is important for cold-responsive gene regulation and cold tolerance in plants.

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ArabidopsisCBF1 and CBF3 have a different function than CBF2 in cold acclimation and define different gene classes in the CBF regulon

Cited by 328 publications

References 33 publications

Induction of Dormancy in Arabidopsis Summer Annuals Requires Parallel Regulation of DOG1 and Hormone Metabolism by Low Temperature and CBF Transcription Factors

Induction of Dormancy in Arabidopsis Summer Annuals Requires Parallel Regulation of DOG1 and Hormone Metabolism by Low Temperature and CBF Transcription Factors

Hybrid mimics and hybrid vigor in Arabidopsis

A DEAD Box RNA Helicase Is Critical for Pre-mRNA Splicing, Cold-Responsive Gene Regulation, and Cold Tolerance inArabidopsis

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