<i>Arabidopsis</i>Cytochrome P450 Monooxygenase 71A13 Catalyzes the Conversion of Indole-3-Acetaldoxime in Camalexin Synthesis

Nafisi, Majse; Goregaoker, Sameer P.; Botanga, Christopher; Glawischnig, Erich; Olsen, Carl Erik; Halkier, Barbara Ann; Glazebrook, Jane

doi:10.1105/tpc.107.051383

Cited by 338 publications

(348 citation statements)

References 71 publications

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“…However, in cat2 sid2 plants, this induction was abolished. A similar expression pattern was observed for two CYP450s involved in camalexin synthesis (Glawischnig, 2007;Nafisi et al, 2007) as well as for camalexin itself and both free and total scopoletin (Fig. 5, C-G).…”

Section: Genetic Reversion Of Pathogen Responses and Gene Expression supporting

confidence: 52%

Peroxisomal Hydrogen Peroxide Is Coupled to Biotic Defense Responses by ISOCHORISMATE SYNTHASE1 in a Daylength-Related Manner

et al. 2010

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While it is well established that reactive oxygen species can induce cell death, intracellularly generated oxidative stress does not induce lesions in the Arabidopsis (Arabidopsis thaliana) photorespiratory mutant cat2 when plants are grown in short days (SD). One interpretation of this observation is that a function necessary to couple peroxisomal hydrogen peroxide (H 2 O 2 )-triggered oxidative stress to cell death is only operative in long days (LD). Like lesion formation, pathogenesis-related genes and camalexin were only induced in cat2 in LD, despite less severe intracellular redox perturbation compared with SD. Lesion formation triggered by peroxisomal H 2 O 2 was modified by introducing secondary mutations into the cat2 background and was completely absent in cat2 sid2 double mutants, in which ISOCHORISMATE SYNTHASE1 (ICS1) activity is defective. In addition to H 2 O 2 -induced salicylic acid (SA) accumulation, the sid2 mutation in ICS1 abolished a range of LD-dependent pathogen responses in cat2, while supplementation of cat2 with SA in SD activated these responses. Nontargeted transcript and metabolite profiling identified clusters of genes and small molecules associated with the daylength-dependent ICS1-mediated relay of H 2 O 2 signaling. The effect of oxidative stress in cat2 on resistance to biotic challenge was dependent on both growth daylength and ICS1. We conclude that (1) lesions induced by intracellular oxidative stress originating in the peroxisomes can be genetically reverted; (2) the isochorismate pathway of SA synthesis couples intracellular oxidative stress to cell death and associated disease resistance responses; and (3) camalexin accumulation was strictly dependent on the simultaneous presence of both H 2 O 2 and SA signals.

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Section: Genetic Reversion Of Pathogen Responses and Gene Expression supporting

confidence: 52%

Peroxisomal Hydrogen Peroxide Is Coupled to Biotic Defense Responses by ISOCHORISMATE SYNTHASE1 in a Daylength-Related Manner

et al. 2010

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“…2E) were among the 54 genes differentially expressed in the three mutants. In addition, three enzymes implicated in synthesis of the phytoalexin camalexin (PAD3, CYP71A13, and CYP79B2; Nafisi et al, 2007), and three genes involved in the synthesis or maintenance of SA (ICS1, EDS1, and PAD4) are shared by mekk1 and mpk4 and are also up-regulated in mkk1/2, although they are not among the 500 most significantly differentially regulated genes in mkk1/2. Gene ontology analysis of the overlapping genes in mkk1/2, mekk1, and mpk4 revealed SAR to be the most significantly overrepresented term among many other terms implicated in defense response (Supplemental Table S4).…”

Section: Mekk1 Mkk1/2 and Mpk4 Exhibit A Gradient Of Phenotypic Sevmentioning

confidence: 99%

Arabidopsis Mitogen-Activated Protein Kinase Kinases MKK1 and MKK2 Have Overlapping Functions in Defense Signaling Mediated by MEKK1, MPK4, and MKS1

et al. 2008

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The Arabidopsis (Arabidopsis thaliana) MKK1 and MKK2 mitogen-activated protein kinase kinases have been implicated in biotic and abiotic stress responses as part of a signaling cascade including MEKK1 and MPK4. Here, the double loss-offunction mutant (mkk1/2) of MKK1 and MKK2 is shown to have marked phenotypes in development and disease resistance similar to those of the single mekk1 and mpk4 mutants. Because mkk1 or mkk2 single mutants appear wild type, basal levels of MPK4 activity are not impaired in them, and MKK1 and MKK2 are in part functionally redundant in unchallenged plants. These findings are confirmed and extended by biochemical and molecular analyses implicating the kinases in jasmonate-and salicylate-dependent defense responses, mediated in part via the MPK4 substrate MKS1. In addition, transcriptome analyses delineate overlapping and specific effects of the kinases on global gene expression patterns demonstrating both redundant and unique functions for MKK1 and MKK2.

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“…However, compounds of that class remained undetectable in avocado tissue, hence the function of CYP71A1 in the plant and the physiological substrate are still unclear [30]. CYP71A13, a member of the largest and most diverse family in Arabidopsis, was shown to be involved in the formation of the indole alkaloid specialized metabolite camalexin [31], suggesting that CYP71A family members have acquired highly specific functions across and within individual species. The elusive P450 with in planta geraniol hydroxylase activity was finally discovered in C. roseus, where CYP76B6 was shown to be involved in the route to monoterpenoid indole alkaloids [32].…”

Section: The Cyp71 Clan: Cradle Of Monoterpenoid and Sesquiterpenoid mentioning

confidence: 99%

Plant P450s as versatile drivers for evolution of species-specific chemical diversity

Hamberger

Bak

2013

Phil. Trans. R. Soc. B

268

231

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The irreversible nature of reactions catalysed by P450s makes these enzymes landmarks in the evolution of plant metabolic pathways. Founding members of P450 families are often associated with general (i.e. primary) metabolic pathways, restricted to single copy or very few representatives, indicative of purifying selection. Recruitment of those and subsequent blooms into multi-member gene families generates genetic raw material for functional diversification, which is an inherent characteristic of specialized (i.e. secondary) metabolism. However, a growing number of highly specialized P450s from not only the CYP71 clan indicate substantial contribution of convergent and divergent evolution to the observed general and specialized metabolite diversity. We will discuss examples of how the genetic and functional diversification of plant P450s drives chemical diversity in light of plant evolution. Even though it is difficult to predict the function or substrate of a P450 based on sequence similarity, grouping with a family or subfamily in phylogenetic trees can indicate association with metabolism of particular classes of compounds. Examples will be given that focus on multi-member gene families of P450s involved in the metabolic routes of four classes of specialized metabolites: cyanogenic glucosides, glucosinolates, mono-to triterpenoids and phenylpropanoids.

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ArabidopsisCytochrome P450 Monooxygenase 71A13 Catalyzes the Conversion of Indole-3-Acetaldoxime in Camalexin Synthesis

Cited by 338 publications

References 71 publications

Peroxisomal Hydrogen Peroxide Is Coupled to Biotic Defense Responses by ISOCHORISMATE SYNTHASE1 in a Daylength-Related Manner

Peroxisomal Hydrogen Peroxide Is Coupled to Biotic Defense Responses by ISOCHORISMATE SYNTHASE1 in a Daylength-Related Manner

Arabidopsis Mitogen-Activated Protein Kinase Kinases MKK1 and MKK2 Have Overlapping Functions in Defense Signaling Mediated by MEKK1, MPK4, and MKS1

Plant P450s as versatile drivers for evolution of species-specific chemical diversity

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