<i>Arabidopsis</i> DPB3-1, a DREB2A Interactor, Specifically Enhances Heat Stress-Induced Gene Expression by Forming a Heat Stress-Specific Transcriptional Complex with NF-Y Subunits

Sato, Hikaru; Mizoi, Junya; Tanaka, Hidenori; Maruyama, Kyonosin; Qin, Feng; Osakabe, Yuriko; Morimoto, Kanehisa; Ohori, Teppei; Kusakabe, Kazuya; Nagata, Maika; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko

doi:10.1105/tpc.114.132928

Cited by 158 publications

(134 citation statements)

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“…The different subunits of NF-Y are specifically expressed in different tissues and organs during special developmental stages (Gusmaroli et al, 2001, 2002; Siefers et al, 2009; Cao et al, 2011; Sorin et al, 2014), or in response to environmental changes (Pant et al, 2009; Des Marais et al, 2012), suggesting that only some combinations of NF-Y subunits can be assembled and act in different developmental stages or under certain stimuli or conditions. In addition, yeast two-hybrid and three-hybrid systems have also been used to detect protein-protein interactions and formation of heterotrimers between Arabidopsis NF-Y subunits (Calvenzani et al, 2012; Hackenberg et al, 2012; Sato et al, 2014). Yeast two-hybrid assays indicated that most of the NF-Y subunits of the same type (A-A, B-B, or C-C) could not form homodimers or heterodimers, and NF-YA and NF-YB show little or no interaction.…”

Section: Main Textmentioning

confidence: 99%

“…Research based on yeast three-hybrid and co-immunoprecipitation assays showed that NF-Y could function as a NF-YA-YB-YC heterotrimeric complex in vivo ( Table 1 ) (Hou et al, 2014; Sato et al, 2014). In theory, Arabidopsis NF-Y subunits can form 1000 different heterotrimeric combinations (Petroni et al, 2012); however, only a few NF-Y complexes have been verified through molecular and biochemical studies ( Table 1 ).…”

Section: Main Textmentioning

confidence: 99%

“…In the first mechanism, the NF-YB-YC heterodimer assembles in the cytoplasm, then translocates into the nucleus, where it interacts with NF-YA to form an active heterotrimer ( Figure 2A ) (Hackenberg et al, 2012; Laloum et al, 2013). The NF-YA-YB-YC complex mainly binds to the CCAAT box in the promoter regions of the downstream target genes through NF-YA, and regulates the expression of target genes, which is a highly conserved transcriptional regulation mechanism for NF-Y complexes in yeast, mammals, and plants (Mantovani, 1999; Frontini et al, 2004; Liu and Howell, 2010; Dolfini et al, 2012; Petroni et al, 2012; Hou et al, 2014; Sato et al, 2014). For example, the AtNF-YA4-YC2-YB3 complex binds to the CCAAT cis -element in the promoter region of BINDING PROTEIN 3 ( BiP3 ) through AtNF-YA4 and up-regulates the expression of ER stress-induced genes (Liu and Howell, 2010).…”

Section: Main Textmentioning

confidence: 99%

“…Further, overexpression of NF-YA1 enhanced plant resistance to salt stress by increasing the expression of Abscisic Acid Insensitive3 ( ABI3 ) and Abscisic Acid Insensitive5 ( ABI5 ; Li et al, 2013). In addition, the NF-YA2-B3-C10 ternary complex enhanced the expression of the heat stress-inducible gene HEAT SHOCK FACTOR A3 ( HsfA3 ) during heat stress responses in cooperation with DREB2A (Sato et al, 2014). …”

Section: Main Textmentioning

confidence: 99%

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The Arabidopsis thaliana Nuclear Factor Y Transcription Factors

et al. 2017

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Nuclear factor Y (NF-Y) is an evolutionarily conserved trimeric transcription factor complex present in nearly all eukaryotes. The heterotrimeric NF-Y complex consists of three subunits, NF-YA, NF-YB, and NF-YC, and binds to the CCAAT box in the promoter regions of its target genes to regulate their expression. Yeast and mammal genomes generally have single genes with multiple splicing isoforms that encode each NF-Y subunit. By contrast, plant genomes generally have multi-gene families encoding each subunit and these genes are differentially expressed in various tissues or stages. Therefore, different subunit combinations can lead to a wide variety of NF-Y complexes in various tissues, stages, and growth conditions, indicating the potentially diverse functions of this complex in plants. Indeed, many recent studies have proved that the NF-Y complex plays multiple essential roles in plant growth, development, and stress responses. In this review, we highlight recent progress on NF-Y in Arabidopsis thaliana, including NF-Y protein structure, heterotrimeric complex formation, and the molecular mechanism by which NF-Y regulates downstream target gene expression. We then focus on its biological functions and underlying molecular mechanisms. Finally, possible directions for future research on NF-Y are also presented.

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Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

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The Arabidopsis thaliana Nuclear Factor Y Transcription Factors

et al. 2017

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“…S5). We further used the Arabidopsis protoplast transient expression system (Sato et al, 2014) to investigate how PaC3H17 fused with SRDX sequence affects PaMYB199 transcription. These results implied that PaC3H17 potentially binds to some element(s) in the P4 fragment of the PaMYB199 promoter.…”

Section: New Phytologistmentioning

confidence: 99%

Dual regulation of xylem formation by an auxin‐mediated PaC3H17‐PaMYB199 module in Populus

et al. 2019

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Summary Wood (secondary xylem) formation in tree species is dependent on auxin‐mediated vascular cambium activity in stems. However, the complex regulatory networks underlying xylem formation remain elusive. Xylem development in Populus was characterized based on microscopic observations of stem sections in transgenic plants. Transcriptomic, quantitative real‐time PCR, chromatin immunoprecipitation PCR, and electrophoretic mobility shift assay analyses were conducted to identify target genes involved in xylem development. Yeast two‐hybrid, pull‐down, bimolecular fluorescence complementation, and co‐immunoprecipitation assays were used to validate protein–protein interactions. PaC3H17 and its target PaMYB199 were found to be predominantly expressed in the vascular cambium and developing secondary xylem in Populus stems and play opposite roles in controlling cambial cell proliferation and secondary cell wall thickening through an overlapping pathway. Further, PaC3H17 interacts with PaMYB199 to form a complex, attenuating PaMYB199‐driven suppression of its xylem targets. Exogenous auxin application enhances the dual control of the PaC3H17‐PaMYB199 module during cambium division, thereby promoting secondary cell wall deposition. Dual regulation of xylem formation by an auxin‐mediated PaC3H17‐PaMYB199 module represents a novel regulatory mechanism in Populus, increasing our understanding of the regulatory networks involved in wood formation.

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Water stress tolerance in maize

Chellamma¹,

Pillai²

2016

Water Stress and Crop Plants

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Arabidopsis DPB3-1, a DREB2A Interactor, Specifically Enhances Heat Stress-Induced Gene Expression by Forming a Heat Stress-Specific Transcriptional Complex with NF-Y Subunits

Cited by 158 publications

References 71 publications

The Arabidopsis thaliana Nuclear Factor Y Transcription Factors

The Arabidopsis thaliana Nuclear Factor Y Transcription Factors

Dual regulation of xylem formation by an auxin‐mediated PaC3H17‐PaMYB199 module in Populus

Water stress tolerance in maize

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