Abstract:Arabidopsis histone H3 lysine 4 (H3K4) demethylases play crucial roles in several developmental processes, but their involvement in seedling establishment remain unexplored.Here, we show that Arabidopsis JUMONJI DOMAIN-CONTAINING PROTEIN17 (JMJ17), an H3K4me3 demethylase, is involved in cotyledon greening during seedling establishment. Dark-grown seedlings of jmj17 accumulated a high concentration of protochlorophyllide, an intermediate metabolite in the tetrapyrrole biosynthesis (TPB) pathway that generates c… Show more
“…JMJ14, JMJ15, and JMJ18 play important roles in regulating flowering by reducing H3K4me3 abundance at various flowering regulatory genes. In addition to regulating the transition to flowering, Arabidopsis demethylases also play roles in RNA silencing ( Searle et al, 2010 ); circadian clock ( Song et al, 2019 ); defense ( Li et al, 2020 ); senescence ( Liu et al, 2019 ); de-etiolation process ( Islam et al, 2021 ), and dehydration stress ( Huang et al, 2019 ). JMJ17 played a role in dehydration stress by directly binding to OST1 and demethylating H3K4me3 levels ( Huang et al, 2019 ).…”
Post-translational histone modifications play important roles in regulating chromatin structure and transcriptional regulation. Histone 3 lysine 4 trimethylation (H3K4me3) is a prominent histone modification mainly associated with gene activation. Here we showed that a histone demethylase, JMJ15, belonging to KDM5/JARID group, is involved in salt stress response in Arabidopsis thaliana. Jmj15 loss-of-function mutants displayed increased sensitivity to salt stress. Moreover, knockout of JMJ15 impaired the salt responsive gene expression program and affected H3K4me3 levels of many stress-related genes under salt-stressed condition. Importantly, we demonstrated that JMJ15 regulated the expression level of two WRKY transcription factors, WRKY46 and WRKY70, which were negatively involved in abiotic stress tolerance. Furthermore, JMJ15 directly bound to and demethylated H3K4me3 mark in the promoter and coding regions of WRKY46 and WRKY70, thereby repressing these two WRKY gene expression under salt stress. Overall, our study revealed a novel molecular function of the histone demethylase JMJ15 under salt stress in plants.
“…JMJ14, JMJ15, and JMJ18 play important roles in regulating flowering by reducing H3K4me3 abundance at various flowering regulatory genes. In addition to regulating the transition to flowering, Arabidopsis demethylases also play roles in RNA silencing ( Searle et al, 2010 ); circadian clock ( Song et al, 2019 ); defense ( Li et al, 2020 ); senescence ( Liu et al, 2019 ); de-etiolation process ( Islam et al, 2021 ), and dehydration stress ( Huang et al, 2019 ). JMJ17 played a role in dehydration stress by directly binding to OST1 and demethylating H3K4me3 levels ( Huang et al, 2019 ).…”
Post-translational histone modifications play important roles in regulating chromatin structure and transcriptional regulation. Histone 3 lysine 4 trimethylation (H3K4me3) is a prominent histone modification mainly associated with gene activation. Here we showed that a histone demethylase, JMJ15, belonging to KDM5/JARID group, is involved in salt stress response in Arabidopsis thaliana. Jmj15 loss-of-function mutants displayed increased sensitivity to salt stress. Moreover, knockout of JMJ15 impaired the salt responsive gene expression program and affected H3K4me3 levels of many stress-related genes under salt-stressed condition. Importantly, we demonstrated that JMJ15 regulated the expression level of two WRKY transcription factors, WRKY46 and WRKY70, which were negatively involved in abiotic stress tolerance. Furthermore, JMJ15 directly bound to and demethylated H3K4me3 mark in the promoter and coding regions of WRKY46 and WRKY70, thereby repressing these two WRKY gene expression under salt stress. Overall, our study revealed a novel molecular function of the histone demethylase JMJ15 under salt stress in plants.
“…In this issue of New Phytologist , Islam et al . (2021; pp. 1023–1039) demonstrate that epigenetic control of histone methylation by JUMONJI DOMAIN‐CONTAINING PROTEIN17 (JMJ17) is another important mechanism plants use to control this transition.‘(An) exciting role for epigenetic gene regulation during the delicate timing of photomorphogenesis and chloroplast biogenesis'.…”
Section: Figmentioning
confidence: 99%
“…Model based on the article published in this issue of New Phytologist by Islam et al . (2021; pp. 1023–1039).…”
All in the timing: epigenetic control of greening During germination, the emerging seedling relies on a limited supply of energy and nutrients stored within itself and the seed. This often occurs underground in darkness where seedlings undergo skotomorphogenesis, characterized by hypocotyl elongation, reduced root growth, and small closed cotyledons forming an apical hook to protect the shoot apical meristem as the seedling grows though the soil towards sunlight (Fig. 1). Such long and pale dark-grown seedlings are commonly referred to as etiolated (from the French eiolier (to make pale)). However, a seedling must eventually find sunlight and make an irreversible shift to photoautotrophic growth (photomorphogenesis or de-etiolation). This leads to a dramatic change in physiology, including reduced hypocotyl elongation, increased root growth, expansion and unfolding of the cotyledons, and the biogenesis of photosynthetic plastids (i.e. chloroplasts). This shift is accompanied by a massive change in gene expression, with about one-third of genes being reprogrammed (Liu et al., 2012). A large amount of the transcriptional response is regulated by the plant photoreceptors (phytochromes and cryptochromes) that activate transcriptional networks. This involves the rapid degradation of PHYTOCHROME INTERACTING FACTORS (PIFs), which are basic helix-loop-helix domain-containing transcription factors that repress photomorphogenesis in the dark (Hern andez-Verdeja et al., 2020). In a recently published article in
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