<i>Arabidopsis</i><scp>RAN GTPases</scp> are critical for mitosis during male and female gametogenesis

Qin, Zheng; Wu, Yanan; Sun, Tao; Ma, Ting; Xu, Meng; Pang, Chao; Li, Shan‐Wei; Li, Sha

doi:10.1002/1873-3468.14422

Cited by 2 publications

(1 citation statement)

References 51 publications

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“…Arabidopsis RANs, the small GTPases, play fundamental roles in pollen mitosis I. Both the dominant negative and constitutively active variants of RAN1 can lead to the developmental stagnation of microgametogenesis at PM I (Qin et al, 2022). SEC6, an exocyst subunit of octameric tethered complexes, is involved in vesicle trafficking to promote cell plate formation in pollen mitosis I in Arabidopsis (Tan et al, 2022).…”

Section: Mitosis Of Microsporesmentioning

confidence: 99%

Male gametogenesis in flowering plants

Cui,

Xu,

Wang

et al. 2024

Front. Sustain. Food Syst.

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The life cycles of plants are characterized by significant alternations between the diploid sporophytic and the haploid gametophytic generations. Meiosis and fertilization are the prerequisites for achieving the alternation of generations. Diploid sporophytes undergo meiosis to produce haploid gametes, and male–female gametes fuse (double fertilization) to revert to the diploid sporophyte. The formation and development of male gametophytes are crucial for the alternation of generations in higher plants. During the long evolution of plants from aquatic to terrestrial, the way of sexual reproduction has undergone subversive innovations. From sperm swimming for fertilization to relying on the precise delivery of pollen tubes to female gametes for the fusion of the male–female gametes, higher requirements are placed on the male gametophytes’ development and fertilization process. The formation of male gametophytes has undergone significant changes to meet the needs for delivery and transportation of the male gametes. With the emergence of more and more evidence, the molecular mechanism underlying male gametophytes’ development, especially the initiation and specialization of germline cells has been better understood. Focusing on the latest studies, we reviewed and elucidated the critical proteins and factors involved in male gametophyte formation, highlighting the decisive role of auxin in archesporial cell specialization and the importance of microspore maturation in pre-mitosis, and analyzed the molecular mechanisms underlying male gametogenesis, with a view to providing insights for further exploration of male gametophytes formation in the future.

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Section: Mitosis Of Microsporesmentioning

confidence: 99%

Male gametogenesis in flowering plants

Cui,

Xu,

Wang

et al. 2024

Front. Sustain. Food Syst.

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The Arabidopsis KASH protein SINE3 is involved in male and female gametogenesis

Moser,

Groves,

Meier

2024

Plant Reprod

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Key message The Arabidopsis KASH protein SINE3 is involved in male and female gametophyte development, likely affecting the first post-meiotic mitosis in both cases, and is required for full seed set. Abstract Linker of nucleoskeleton and cytoskeleton (LINC) complexes are protein complexes spanning the inner and outer membranes of the nuclear envelope (NE) and are key players in nuclear movement and positioning. Through their roles in nuclear movement and cytoskeletal reorganization, plant LINC complexes affect processes as diverse as pollen tube rupture and stomatal development and function. KASH proteins are the outer nuclear membrane component of the LINC complex, with conserved C-termini but divergent N-terminal cytoplasmic domains. Of the known Arabidopsis KASH proteins, SUN-INTERACTING NUCLEAR ENVELOPE PROTEIN 3 (SINE3) has not been functionally characterized. Here, we show that SINE3 is expressed at all stages of male and female gametophyte development. It is located at the NE in male and female gametophytes. Loss of SINE3 results in a female-derived seed set defect, with sine3 mutant ovules arresting at stage FG1. Pollen viability is also significantly reduced, with microspores arresting prior to pollen mitosis I. In addition, sine3 mutants have a minor male meiosis defect, with some tetrads containing more than four spores. Together, these results demonstrate that the KASH protein SINE3 plays a crucial role in male and female gametophyte development, likely affecting the first post-meiotic nuclear division in both cases.

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ArabidopsisRAN GTPases are critical for mitosis during male and female gametogenesis

Cited by 2 publications

References 51 publications

Male gametogenesis in flowering plants

Male gametogenesis in flowering plants

The Arabidopsis KASH protein SINE3 is involved in male and female gametogenesis

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