Arabidopsis thaliana H1 histones

Abstract

We have isolated two Arubidopsis thulium cDNA clones that encode different H1 histone proteins. The H1-3 and HI-2 proteins are 274 and 273 amino acids in length, respectively. Unlike the H1 histones within a single animal species, the two plant H1 proteins share little sequence similarity outside the protein's central globular domain. Within the globular domain, a pentapeptide that is extremely well conserved in animal Hl histones, is not found in either of the plant proteins. Southern blot analysis suggests that A. thalianu has only three HI histone genes. A genomic clone encoding the HI-1 protein was isolated and the protein-coding region was found to consist of two exons separated by a 104-bp intron. The site of transcriptional initiation of the H1-I gene was mapped by primer-extension analysis and a conserved octamer motif, identical to that observed in most plant core histone genes that have been characterized to date, was found 101 nucleotides upstream of the presumed transcription-initiation site. The 3' portion of the gene encoding H1-2 was also isolated and sequenced. When the 3'-flanking regions of the two H1 genes were compared, several highly conserved sequences were observed that might be convergently transcribed relative to the histone genes.

Molecular cloning, sequence analysis and differential expression of an intron‐containing gene encoding tomato histone H1

“…In general, apicies and young tissues contain significant amounts of mRNA, but mature tissues do not. We found that the histone H1 mRNA is a polyadenylated message (data not shown) and, in this respect, it is similar to all the other reported higher plant H1 mRNAs [9,10,21,291. In contrast, the Volvox [20] and most animal cell histone H1 mRNAs are non-polyadenylated, and those which are generally fit into the category of developmental variants or replication-independent variants.…”

Linker Histones Play a Role in Male Meiosis and the Development of Pollen Grains in Tobacco

“…None of the six bands was recognized by antibodies raised against the tobacco high-mobility group HMG1 protein (results not shown). The sizes of the two major bands (H1A and H1B) corresponded well to that of the typical major somatic plant H1 (equivalent to 250 to 280 amino acid residues; Gantt and Lenvik, 1991) and to the size predicted from the single histone H1 cDNA sequence that has been reported from tobacco, which encodes a protein of 282 amino acids (Szekeres et al, 1995). To determine whether this cDNA sequence codes for H1A or H1B, we used it as a template in an in vitro transcription/translation assay.…”

Deletion of the unique gene encoding a typical histone H1 has no apparent phenotype in Aspergillus nidulans

“…The hhoA mRNA is polyadenylated, as already indicated by the fact that several cDNA fragments have been cloned using anchor-dT as a primer for the ®rst strand synthesis. Poly(A)-containing transcripts have been described for histone genes from a number of organisms (Ehinger et al, 1990;Sanicola et al, 1990;Gannt and Lenvik, 1991;Ushinsky et al, 1997). The sequence of two independent cDNA clones shows a polyadenylation site at position 1528 (see Fig.…”