<i>Arabidopsis thaliana </i><scp>NIP</scp>7;1 is involved in tissue arsenic distribution and tolerance in response to arsenate

Lindsay, Emma Rebecca; Maathuis, Frans J. M.

doi:10.1002/1873-3468.12103

Cited by 45 publications

(19 citation statements)

References 23 publications

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“…AtTIP5;1 improved plant tolerance to boron toxicity [38], and its homologous gene GmTIP5;1 might also confer resistance to boron. AtNIP7;1 enhanced tolerance to arsenate toxicity [39], and its homologous genes ( GmNIP7;1 and GmNIP7;2 ) might also contribute to arsenate stress. Compared with previous report [28], 17 GmAQP proteins were newly identified which were distributed in PIP, TIP, NIP and XIP sub-families: 11 GmPIPs (GmPIP1;9, GmPIP1;10, GmPIP2;3, GmPIP2;4, GmPIP2;8, GmPIP2;9, GmPIP2;10, GmPIP2;11, GmPIP2;12, GmPIP2;13, and GmPIP2;14), 1 GmTIP (GmTIP1;10), 4 GmNIPs (GmNIP1;6, GmNIP3;1, GmNIP4;1, and GmNIP4;2) and 1 GmXIP (GmXIP1;2).…”

Section: Discussionmentioning

confidence: 99%

Investigation of the AQP Family in Soybean and the Promoter Activity of TIP2;6 in Heat Stress and Hormone Responses

Zheng

Liu

Zhang

et al. 2019

IJMS

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Aquaporins (AQPs) are one diverse family of membrane channel proteins that play crucial regulatory roles in plant stress physiology. However, the heat stress responsiveness of AQP genes in soybean remains poorly understood. In this study, 75 non-redundant AQP encoding genes were identified in soybean. Multiple sequence alignments showed that all GmAQP proteins possessed the conserved regions, which contained 6 trans-membrane domains (TM1 to TM6). Different GmAQP members consisted of distinct Asn-Pro-Ala (NPA) motifs, aromatic/arginine (ar/R) selectivity filters and Froger’s positions (FPs). Phylogenetic analyses distinguished five sub-families within these GmAQPs: 24 GmPIPs, 24 GmTIPs, 17 GmNIPs, 8 GmSIPs, and 2 GmXIPs. Promoter cis-acting elements analyses revealed that distinct number and composition of heat stress and hormone responsive elements existed in different promoter regions of GmAQPs. QRT-PCR assays demonstrated that 12 candidate GmAQPs with relatively extensive expression in various tissues or high expression levels in root or leaf exhibited different expression changes under heat stress and hormone cues (abscisic acid (ABA), l-aminocyclopropane-l-carboxylic acid (ACC), salicylic acid (SA) and methyl jasmonate (MeJA)). Furthermore, the promoter activity of one previously functionally unknown AQP gene-GmTIP2;6 was investigated in transgenic Arabidopsis plants. The beta-glucuronidase (GUS) activity driven by the promoter of GmTIP2;6 was strongly induced in the heat- and ACC-treated transgenic plants and tended to be accumulated in the hypocotyls, vascular bundles, and leaf trichomes. These results will contribute to uncovering the potential functions and molecular mechanisms of soybean GmAQPs in mediating heat stress and hormone signal responses.

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Section: Discussionmentioning

confidence: 99%

Investigation of the AQP Family in Soybean and the Promoter Activity of TIP2;6 in Heat Stress and Hormone Responses

Zheng

Liu

Zhang

et al. 2019

IJMS

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“…Numerous studies of bidirectional arsenic channels have also been established within the NIP subfamily of Arabidopsis, rice, and lotus [19,[45][46][47]. In addition to uptake, a recent study suggests that NIPs are also involved in As (III) translocation and distribution from roots to the distal parts of the plant [48].…”

Section: Metalloidsmentioning

confidence: 99%

Molecular and Functional Characterization of Grapevine NIPs through Heterologous Expression in aqy-Null Saccharomyces cerevisiae

Sabir

Gomes

Loureiro-Dias

et al. 2020

IJMS

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Plant Nodulin 26-like Intrinsic Proteins (NIPs) are multifunctional membrane channels of the Major Intrinsic Protein (MIP) family. Unlike other homologs, they have low intrinsic water permeability. NIPs possess diverse substrate selectivity, ranging from water to glycerol and to other small solutes, depending on the group-specific amino acid composition at aromatic/Arg (ar/R) constriction. We cloned three NIPs (NIP1;1, NIP5;1, and NIP6;1) from grapevine (cv. Touriga Nacional). Their expression in the membrane of aqy-null Saccharomyces cerevisiae enabled their functional characterization for water and glycerol transport through stopped-flow spectroscopy. VvTnNIP1;1 demonstrated high water as well as glycerol permeability, whereas VvTnNIP6;1 was impermeable to water but presented high glycerol permeability. Their transport activities were declined by cytosolic acidification, implying that internal-pH can regulate NIPs gating. Furthermore, an extension of C-terminal in VvTnNIP6;1M homolog, led to improved channel activity, suggesting that NIPs gating is putatively regulated by C-terminal. Yeast growth assays in the presence of diverse substrates suggest that the transmembrane flux of metalloids (As, B, and Se) and the heavy metal (Cd) are facilitated through grapevine NIPs. This is the first molecular and functional characterization of grapevine NIPs, providing crucial insights into understanding their role for uptake and translocation of small solutes, and extrusion of toxic compounds in grapevine.

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“…As (V) may be loaded into the xylem via phosphate transporters, such as PHO1, but pho1 mutants did not show decreased As accumulation in the shoots compared with WT plants (Quaghebeur & Rengel, 2004), suggesting that As(III) is the main As species loaded into the xylem in WT plants. As(III) can be loaded into the xylem via NIP3;1 and NIP7;1 in A. thaliana (Xu et al, 2015;Lindsay & Maathuis, 2016). In rice, the silicic acid efflux transporter Lsi2 is also able to transport As(III) out of the cells towards the stele for xylem loading (Ma et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Dissecting the components controlling root‐to‐shoot arsenic translocation in Arabidopsis thaliana

Wang

Bermejo

et al. 2017

New Phytologist

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Summary • Arsenic (As) is an important environmental and food-chain toxin. We investigated the key components controlling As accumulation and tolerance in Arabidopsis thaliana. • We tested the effects of different combinations of gene knockout, including arsenate reductase (HAC1), γ-glutamyl-cysteine synthetase (γ-ECS), phytochelatin synthase (PCS1) and phosphate effluxer (PHO1), and the heterologous expression of the As-hyperaccumulator Pteris vittata arsenite efflux (PvACR3), on As tolerance, accumulation, translocation and speciation in A. thaliana. • Heterologous expression of PvACR3 markedly increased As tolerance and root-to-shoot As translocation in A. thaliana, with PvACR3 being localized to the plasma membrane. Combining PvACR3 expression with HAC1 mutation led to As hyperaccumulation in the shoots, whereas combining HAC1 and PHO1 mutation decreased As accumulation. Mutants of γ-ECS and PCS1 were hypersensitive to As and had higher root-to-shoot As translocation. Combining γ-ECS or PCS1 with HAC1 mutation did not alter As tolerance or accumulation beyond the levels observed in the single mutants. • PvACR3 and HAC1 have large effects on root-to-shoot As translocation. Arsenic hyperac cumulation can be engineered in A. thaliana by knocking out the HAC1 gene and expressing PvACR3. PvACR3 and HAC1 also affect As tolerance, but not to the extent of γ-ECS and PCS1.

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Arabidopsis thaliana NIP7;1 is involved in tissue arsenic distribution and tolerance in response to arsenate

Cited by 45 publications

References 23 publications

Investigation of the AQP Family in Soybean and the Promoter Activity of TIP2;6 in Heat Stress and Hormone Responses

Investigation of the AQP Family in Soybean and the Promoter Activity of TIP2;6 in Heat Stress and Hormone Responses

Molecular and Functional Characterization of Grapevine NIPs through Heterologous Expression in aqy-Null Saccharomyces cerevisiae

Dissecting the components controlling root‐to‐shoot arsenic translocation in Arabidopsis thaliana

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