<i>Aralia cordata</i> Inhibits Triacylglycerol Biosynthesis in HepG2 Cells

Kim, Mun Ock; Lee, Sun Hwa; Seo, Jee Hee; Kim, Il Soon; Han, Ah Reum; Moon, Dong Oh; Cho, Sungchan; Cui, Long; Kim, Jung-Woo; Lee, Hyun Sun

doi:10.1089/jmf.2012.2636

Cited by 7 publications

(4 citation statements)

References 33 publications

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“…Some scholars found that the chemical composition of this plant is rich in saponins [21]. Relevant studies have also confirmed that the saponins from the plants in the same genus have antiinflammatory [22,23], anticancer [24][25][26], antiatherosclerosis [27][28][29][30], and antidiabetes [31,32] effects. However, so far, no study has reported on the effect of AAS (root bark) on intimal hyperplasia after a vascular injury.…”

Section: Introductionmentioning

confidence: 88%

Aralia armata (Wall.) Seem Improves Intimal Hyperplasia after Vascular Injury by Downregulating the Wnt3α/Dvl-1/β-Catenin Pathway

Zhao¹,

Huang

et al. 2021

BioMed Research International

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The aim of the study is to examine the mechanism of Aralia armata (Wall.) Seem (AAS) in improving intimal hyperplasia after vascular injury in rats. Rats with femoral artery injury were randomly divided into three groups: the model group, AAS low-dose group (40 mg/kg), and AAS high-dose group (80 mg/kg). The sham operation group was used as a control group. HE staining was used to observe the changes in femoral artery vessels. Immunohistochemistry was adopted to detect α-SMA, PCNA, GSK-3β, and β-catenin proteins in femoral artery tissue. The CCK-8 test and wound healing assay were employed to analyze the effect of AAS on proliferation and migration of vascular smooth muscle cells (VSMCs) cultured in vitro. Western blotting (WB) and polymerase chain reaction (PCR) assays were used to evaluate the molecular mechanism. AAS reduced the stenosis of blood vessels and the protein expressions of α-SMA, PCNA, GSK-3β, and β-catenin compared to the model group. In addition, AAS (0-15 μg/mL) effectively inhibited the proliferation and migration of VSMCs. Moreover, the results of WB and PCR showed that AAS could inhibit the activation of β-catenin induced by 15% FBS and significantly decrease the expression levels of Wnt3α, Dvl-1, GSK-3β, β-catenin, and cyclin D1 in the upstream and downstream of the pathway. AAS could effectively inhibit the proliferation and migration of neointima after vascular injury in rats by regulating the Wnt/β-catenin signaling pathway.

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Section: Introductionmentioning

confidence: 88%

Aralia armata (Wall.) Seem Improves Intimal Hyperplasia after Vascular Injury by Downregulating the Wnt3α/Dvl-1/β-Catenin Pathway

Zhao¹,

Huang

et al. 2021

BioMed Research International

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“…GPAT has been recognised as one of the drug targets for the treatment of metabolic syndrome (Ref. 57). With the improvement of living standards, the incidence of metabolic syndrome continues to increase.…”

Section: Conclusion and Future Outlookmentioning

confidence: 99%

Glycerol-3-phosphate acyltransferases and metabolic syndrome: recent advances and future perspectives

Huang

Lin

et al. 2022

Expert Rev. Mol. Med.

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Triglycerol-3-phosphate acyltransferases (GPATs) are the key enzymes in the first step of the synthesis of triacylglycerol (TAG). In mammals, there are four isoforms of GPATs. GPAT1 and GPAT2 are localised in the outer mitochondrial membrane, while GPAT3 and GPAT4 are localised in the endoplasmic reticulum. Previous research has emphasised that GPAT plays a critical effect on the development of metabolic syndromes, such as liver steatosis, obesity, and insulin resistance. In this review, we will critically evaluate the regulatory effects of GPATs isoforms in metabolic syndrome. In addition, we also discuss perspectives on clinical intervention strategies for the neurometabolic disease.

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“…-растение, внесенное в Красную книгу России [2]. Вторичные метаболиты, входящие в состав сырья аралии, оказывают антибактериальное [3], гиполипидемическое [4], обезболивающее [5], противовоспалительное [6], адаптогенное [7] и др. [8] действие.…”

Section: Introductionunclassified

Callus Culture of <i>Aralia cordata</i> Thunb.: Obtaining, Selection of Cultivating Conditions, Somatic Embryogenesis Induction

Nekrasova,

Povydysh,

Pivovarova

et al. 2023

Razrabotka i registraciâ lekarstvennyh sredstv

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Introduction. Cell cultures spreads widely in different areas of biology, biotechnology and agriculture. Aralia cordata is a perennial herbaceous plant, which has been listed in Red book of the Russian Federation. Pharmaceuticals which are based on raw materials of Aralia ssp. have valuable types of pharmacological activity and are widely used in oriental medicine. A serious obstacle for resumption of the natural populations is the presence of the period of morphophysiological dormancy in the seeds of the plants, which requires a long-time stratification process. Limitation of the natural geographic range and the combination of biological activities useful for humans make Aralia cordata Thunb. prospective object for in vitro introduction.Aim. The aim of the study is obtaining of the viable cell culture of Aralia cordata Thunb., investigation of the somatic embryogenesis conditions.Materials and methods. Pieces of the leaves of Aralia cordata intact plant from Komarov Botanical Institute of the Russian Academy of Sciences were used as a primary explants. Pieces of leaves were sterilized in a 2 % benzalkonium chloride solution for 5 minutes, induction of primary callogenesis was carried out on Murasige – Skoog medium. Nutrient media with different constituents were discovered for choosing one for long-time cultivation of calli. Induction of somatic embryogenesis was carried out on the nutrient media with high auxins content. Ethanol extracts from the intact plant and calli cultures were assayed with HPTLC PRO SYSTEM (CAMAG AG, Switzerland).Results and discussion. After two weeks of cultivation, the formation of primary callus was observed on the surface of the explants. The Linsmaier – Skoog medium with a reduced amount of sucrose (20 g/l) was recognized as the most suitable medium for long-term maintenance of cultures. Embryoid structures of Aralia cordata have been obtained, now we are continuing to collect analytics data about this process. Qualitative analysis of the extracts showed that callus cultures accumulate triterpene glycosides, their composition is close to that of the intact plant.Conclusion. A viable strain of callus culture of Aralia cordata Thunb. was obtained, a nutrient medium for long-term cultivation of calli was established. Somatic embryoids have been obtained, and their further development is currently being monitored. A preliminary phytochemical study showed that the composition of the chemical components of calli is close to that of an intact plant.

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Aralia cordata Inhibits Triacylglycerol Biosynthesis in HepG2 Cells

Cited by 7 publications

References 33 publications

Aralia armata (Wall.) Seem Improves Intimal Hyperplasia after Vascular Injury by Downregulating the Wnt3α/Dvl-1/β-Catenin Pathway

Aralia armata (Wall.) Seem Improves Intimal Hyperplasia after Vascular Injury by Downregulating the Wnt3α/Dvl-1/β-Catenin Pathway

Glycerol-3-phosphate acyltransferases and metabolic syndrome: recent advances and future perspectives

Callus Culture of <i>Aralia cordata</i> Thunb.: Obtaining, Selection of Cultivating Conditions, Somatic Embryogenesis Induction

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