Artemisia sphaerocephala Krasch polysaccharide promotes adipose thermogenesis and decreases obesity by shaping the gut microbiota

Abstract

This study was designed to investigate the underlying mechanism of Artemisia sphaerocephala Krasch polysaccharide (ASKP) against obesity. Here, our results showed that ASKP considerably reduced body weight gain and metabolic...

Polysaccharide from Ziyang Selenium-Enriched Green Tea Prevents Obesity and Promotes Adipose Thermogenesis via Modulating the Gut Microbiota

“…Several studies have evidenced that disturbance of host gut microbiota is a vital factor in obesity as well as other diseases. , The 16S rRNA gene sequencing technology was used to explore the regulatory effect of Se-GTP on gut microbiota. As shown in the Venn diagram (Figure A), 233 cases of ASV were detected in the five groups, and the specific ASV numbers of the NC group, HFD group, and three Se-GTP intervention groups were 482, 162, 205, 219, and 370, respectively.…”

“…Obesity is the basis of some chronic diseases, which develops owing to various factors, such as diet, gene, lifestyle, intestinal microbiota, and unbalanced energy metabolism. − Recent studies suggest that increasing energy expenditure is a valid approach to treat obesity, among which boosting adaptive BAT thermogenesis and WAT browning have the potential to be the most attractive strategy for treating obesity and related metabolic disorders. , Indeed, our previous study has shown that the use of Artemisia sphaerocephala Krasch polysaccharide, which can promote BAT thermogenesis, could improve metabolic disorders in the obese model . In this study, by integrating data on gut microbiota and BAT, we proved that Se-GTP contributed to a shift in the gut microbiota composition, which stimulated WAT browning, promoted BAT thermogenesis, and subsequently ameliorated HFD causing obesity and related complications.…”

“…In the week before the mice were sacrificed, OGTT and ITT were conducted after fasting for 10 h. Briefly, mice were given 2.0 g/kg glucose orally or intraperitoneally injected with 0.625 U/kg insulin. Then, blood glucose was determined at 0, 15, 30, 60, 90, and 120 min using a glucometer …”

“…After drying the PBS solution, sections were incubated with the secondary antibody for 50 min and then washed three times. Finally, H&E, Oil Red O, and IHC images were photographed under an Axio Imager upright microscope (ZEISS) …”

“…Finally, the supernatant was filtered with a 0.22 μm PVDF membrane and quantitated by UPLC-QqQ-MS (Agilent). UPLC-QqQ-MS analysis was performed according to the conditions established in our group formerly . The mobile phases were 0.1% formic acid water (A) and methanol (B), and the gradient elution program was built as follows: 0–2.5 min, 5% B; 2.5–7.5 min, 5%–90% B; 7.5–10 min, 90% B; 10–12.5 min, 90%–10% B; 12.5–15 min, 10% B, flow rate: 0.3 mL·min –1 , column temperature: 40 °C, and injection volume: 1 μL.…”