<i>Aspergillus fumigatus</i>exoβ(1‐3)glucanases family GH55 are essential for conidial cell wall morphogenesis

Millet, Nicolas; Moya‐Nilges, Maryse; Sachse, Martin; Locker, Jacomina Krijnse; Latgé, Jean‐Paul; Mouyna, Isabelle

doi:10.1111/cmi.13102

Cited by 15 publications

(15 citation statements)

References 53 publications

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“…The analysis of the germination of each conidium in conidial chains was also very informative. Such observation was facilitated in β1,3 glucanase mutants [46], which had defects in conidial separation. Therefore, during germination of these conidial chains, all conidia germinated differently, irrespectively of the age of the conidium (Figure 9).…”

Section: Trying To Modify the Asynchrony Of Conidial Germination In Afumigatusmentioning

confidence: 97%

Aspergillus fumigatus, One Uninucleate Species with Disparate Offspring

Danion

Rhijn

Dufour

et al. 2021

JoF

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Establishment of a fungal infection due to Aspergillus fumigatus relies on the efficient germination of the airborne conidia once they penetrate the respiratory tract. However, the features of conidial germination have been poorly explored and understood in this fungal species as well as in other species of filamentous fungi. We show here that the germination of A. fumigatus is asynchronous. If the nutritional environment and extensive gene deletions can modify the germination parameters for A. fumigatus, the asynchrony is maintained in all germinative conditions tested. Even though the causes for this asynchrony of conidial germination remain unknown, asynchrony is essential for the completion of the biological cycle of this filamentous fungus.

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Section: Trying To Modify the Asynchrony Of Conidial Germination In Afumigatusmentioning

confidence: 97%

Aspergillus fumigatus, One Uninucleate Species with Disparate Offspring

Danion

Rhijn

Dufour

et al. 2021

JoF

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“…Therefore, the coordination of these CAZymes in the network (including GH43, hemicellulose degrading CAZymes) and their presence in the xylem sap implicates a role in fungal colonization, most probably by degrading complex sugars into simpler sugar forms for growth and morphogenesis. Another CAZymes, i.e., GH55, although not directly reported to be involved in the main network, but was observed to be linked with a GH17 family, perhaps contributing to the phenomenon of cell morphogenesis and conidiation (Millet et al 2018(Millet et al , 2019, which is further connected to GH32 (invertase), an enzyme involves in breaking down of sucrose sugar abundantly found in plants.…”

Section: Discussionmentioning

confidence: 99%

“…The secretory CAZymes reported to be involved in fungal cell wall degradation, GH55 (β-1,3-glucanase) seemed to have a role in cell wall morphogenesis and germination (Millet et al 2019). Moreover, an important family and much explored CAZyme, GH18 (Chitinase), identi ed in the present study signi ed its role in morphogenesis, defence, or parasitism (Oyeleye and Normi 2018) because of its involvement in the breakdown of chitin, an essential component of the fungal cell wall for cell growth and plasticity (Langner et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Computational proteomics analysis reveals pathogen secreted carbohydrate active enzymes in tomato xylem sap

Roy¹,

Kalita²,

Jayaprakash³

et al. 2021

Preprint

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Fusarium oxysporum f. sp. lycopersici (Fol), a causal organism of Fusarium wilt in the tomato plant, secretes cell wall degrading enzymes, also known as carbohydrate-active enzymes (CAZymes). These are crucial during colonization and pathogenesis, as evidenced by several proteomic studies, revealing the importance of these CAZymes in virulence and pathogenicity. However, few of them have been done in-planta, exhibiting differences in the expression of these cell wall degrading enzymes compared to in-vitro studies. Therefore, to explore the CAZymes involved in pathogenesis while residing in the host plant, an in-planta (xylem sap) proteomics of a susceptible tomato variety affected with Fol was done. Most of these CAZymes belonged to the hydrolase and oxidoreductase families having no significant homology with tomato proteins. Nearly 90% of them were predicted to be soluble and extracellular. The core CAZymes families with interactional evidence identified were AA3, GH3, GH18, GH20, GH28, GH43, GH47, GH55 and CE8. Thus, apart from annotating some of the hypothetical proteins to be CAZymes, the study sheds light on CAZymes families that may have a role in the pathogenesis and survival of this fungus in the susceptible tomato plant.

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“…The cell wall of fungi is predominantly composed of fibrillar and branched β-(1,3)-glucan linked to chitin. β-(1,3)-glucanases are essential for proper conidial cell wall morphogenesis and assembly, and segregation of conidia during conidiation and cell wall in A. fumigatus ( Mouyna et al, 2016 ; Millet et al, 2019 ). Thus, the alterations in the Δ Uvsun1 mutants regarding morphology of hyphae and conidia, may be consequences of an altered cell wall.…”

Section: Discussionmentioning

confidence: 99%

SUN-Family Protein UvSUN1 Regulates the Development and Virulence of Ustilaginoidea virens

Cao

et al. 2021

Front. Microbiol.

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Ustilaginoidea virens, the causal agent of rice false smut disease, is an important plant pathogen that causes severe quantitative and qualitative losses in rice worldwide. UvSUN1 is the only member of Group-I SUN family proteins in U. virens. In this work, the role of UvSUN1 in different aspects of the U. virens biology was studied by phenotypic analysis of Uvsun1 knockout strains. We identified that UvSUN1 was expressed during both conidial germination and the infection of rice. Disruption of the Uvsun1 gene affected the hyphal growth, conidiation, morphology of hyphae and conidia, adhesion and virulence. We also found that UvSUN1 is involved in the production of toxic compounds, which are able to inhibit elongation of the germinated seeds. Moreover, RNA-seq data showed that knockout of Uvsun1 resulted in misregulation of a subset of genes involved in signal recognition and transduction system, glycometabolism, cell wall integrity, and secondary metabolism. Collectively, this study reveals that Uvsun1 is required for growth, cell wall integrity and pathogenicity of U. virens, thereby providing new insights into the function of SUN family proteins in the growth and pathogenesis of this pathogen.

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Aspergillus fumigatusexoβ(1‐3)glucanases family GH55 are essential for conidial cell wall morphogenesis

Cited by 15 publications

References 53 publications

Aspergillus fumigatus, One Uninucleate Species with Disparate Offspring

Aspergillus fumigatus, One Uninucleate Species with Disparate Offspring

Computational proteomics analysis reveals pathogen secreted carbohydrate active enzymes in tomato xylem sap

SUN-Family Protein UvSUN1 Regulates the Development and Virulence of Ustilaginoidea virens

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