<i>Aspergillus</i>Diversity Associated with Fungal Diseases on Fish with Molecular Based

Sarjito, Sarjito; Haditomo, Alfabetian Harjuno Condro; Sabdaningsih, Aninditia; Desrina,; Prayitno, Slamet Budi

doi:10.1088/1755-1315/246/1/012035

Cited by 6 publications

(5 citation statements)

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“…The identification of A. lanosus in this study was consistent with a report that Aspergillus sp. has a role of a pathogen in farmed fishes (Chauhan, 2013; Olufemi & Roberts, 1983; Sarjito et al ., 2019). With the increasing use of plant‐derived fodder such as soybean meal and various cereal grains in aquaculture, fish are more susceptible to the potential risk of fungal infection than before (Matejova et al ., 2016).…”

Section: Discussionmentioning

confidence: 99%

Molecular identification and phylogenetic analysis of fungal pathogens isolated from diseased fish in Xinjiang, China

Zhang

Han

et al. 2021

Journal of Fish Biology

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The outbreaks of fungal diseases in cultured fish have been severe in recent years, which is harmful to the healthy and sustainable development of fish farming. In this study, an investigation was conducted for significant fungal infections of 12 species of fish in four regions in Xinjiang, China, to understand the distribution of local fish fungal pathogens. Twenty‐six fungal strains with pathogenicity were isolated, and the challenge experiment showed that eight strains from Changji area had high infection rate to fish eggs. Based on internal transcribed spacer sequence data and molecular analysis, the 26 strains were classified into nine different species of six fungal genera. Phylogenetic analysis showed that all strains were divided into two clades, namely Cluster 1 (contains only the genus Mucor) and Cluster 2 (consists of five small branches), and the distribution of strains from the same region was scattered in two clusters. There is no strict host selectivity for these fungi to infect fish. Mucor sp. are the main fungal pathogen of fish in these four regions, whereas Hypophthalmichthys molitrix and Carassius auratus are two types of fish that were susceptible to pathogen. In addition, the environmental adaptability experiments showed that eight highly pathogenic strains have different adaptability to the environment, and their optimum temperature and pH were 25°C and 7.0, respectively, whereas the concentration of NaCl was negatively correlated with the growth of strains. Therefore, these results indicated that the coinfection of multiple fungal pathogens in a culture region should be considered in the future study.

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Section: Discussionmentioning

confidence: 99%

Molecular identification and phylogenetic analysis of fungal pathogens isolated from diseased fish in Xinjiang, China

Zhang

Han

et al. 2021

Journal of Fish Biology

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“…The molecular detection of Penicillium spp., all isolates amplified using primers ITS1 and ITS4 to a single band of about 550 bp except two species, Penicillium variable showed slight increases band about 565 bp in size, whereas Penicillium rugulosum showed a band of size 540 bp, which was lowest in our study. Similarly, Tiwari et al [31], stated that the consensus primers ITS1 and ITS4 were used to amplify a region of the rDNA gene repeat unit. A recent study of Penicillium isolates based on the banding pattern of the ITS region has shown a more similarity among different species of Penicillium.…”

Section: Discussionmentioning

confidence: 99%

Incidence and molecular characterization of fungi and yeast isolated from cultured catfish and Nile tilapia.

Hashem¹,

Zaki

Adawy³

2020

Mansoura Veterinary Medical Journal

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To study the incidence and seasonal dynamics of different fungi affecting freshwater fishes in Lake Manzala as well as molecular identification of the isolated fungi. Animals: 300 Nile tilapia (Oreochromis niloticus) and 300 catfish (Clarias gariepinus). Design: Descriptive study. Procedures: Random samples of Oreochromis niloticus (O. niloticus) and Clarias gariepinus (C. gariepinus) were collected from Manzala fish farms. Clinical and postmortem examination of fish was applied. Isolation and identification of different fungi were performed by conventional methods. Furthermore, the molecular characterization of isolated fungi was carried out. Results: C. gariepinus had a higher rate of infection with different fungal species than O. niloticus. Aspergillus spp. (Aspergillus niger and Aspergillus flavus) were the most fungal isolated from the examined fishes, followed by Penicillium spp. and Candida albicans. Aspergillus spp were detected in all seasons with a higher rate in summer and spring. A. flavus, A. niger, Penicillium spp. and C.albicans isolates were amplified from both C. gariepinus and O. niloticus at the specified molecular weight using PCR. Conclusion and clinical relevance:Fungal infection affected the fish showing different external and internal lesions, all species of Aspergillus were found in all seasons with a high rate in, hot seasons, summer and spring. The Prevalence of Penicillium and C. albicans were also reported. All fungal isolates were identified on the phenotypic and molecular bases.

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“…The most common aquatic fungi that have been reported in African aquatic systems belong to the hyphomycetes kingdom (Magwaza, Nxumalo, Mamba & Msagati, 2017). To our knowledge, fungal infections can be superficial that invade fish skin (Mahboub & Shaheen, 2021; Parveen & Bari, 2018; Sarjito, 2019; Younis, Esawy, Elkenany & Deen, 2020) or systemic that result in internal infections (Mahboub & Shaheen, 2020; Tartor, Taha, Mahboub & El Ghamery, 2018; Younis et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…are associated with diseased conditions and mortalities in different fish species in various regions including ornamental fish in Egypt and Pakistan, Rainbow trout in Iran, Clarias batrachu in Bhopal, fish hatchery farms in Nigeria, many freshwater fishes in Bihar, African catfish in Ethiopia, farmed fishes in the Province in Iraq, Clarius batrachus in India and many freshwater Fishes in India as well as African catfish in Egypt (Abolude, Opabunmi & Davies, 2013; Chauhan, 2013; Iqbal & Sajjad, 2013; Kumari & Kumar, 2015; Kumari & Sivakami, 2018; Melaku;, Lakew, Alemayehu, Wubie & Chane, 2017; Parveen & Bari, 2018; Refai, Laila, Mohamed & Shimaa, 2010; Shahbazian, Ebrahimzadeh, Soltani, Khosravi & Sharifpour, 2010; Touhali, 2018; Younis et al, 2020). Aspergillosis is a causative agent for early ulcerative syndrome (Podeti & Benarjee, 2015) besides systemic infections and severe epidermal lesions (Sarjito et al, 2019). Aspergillus spp.…”

Section: Introductionmentioning

confidence: 99%

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Detection, diagnosis, Koch’s postulate, hepatorenal and antioxidant indicators for some systemic pathogenic fungi invading the liver and kidneys of African catfish ( Clarias gariepinus ) in Egypt with a histopathological approach

Mahboub

Nada

Abdel‐Ghany

et al. 2022

Aquaculture Research

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Recently, systemic fungal infections have emerged as significant causes of fish morbidity and mortality. Hence, the current study investigates the occurrence of some pathogenic systemic fungi inducing severe lesions in the liver and kidneys of African catfish. Two hundred diseased African catfish were collected from private fish farms in Egypt, and only 120 fish were infected with 212 different fungal isolates at a rate of 60%. The seasonal survey revealed isolation of 72 isolates of yeast (Candida albicans) and 140 isolates of moulds including non‐pigmented moulds, Aspergillus ochraceous and Aspergillus flavus, as well as pigmented mould (Cladosporium herbarum). The isolated fungi induced characteristic post‐mortem lesions. To date, this is the first report for isolation of C. herbarum and C. parapsilosis from Nile catfish liver and kidneys. Various techniques were performed to identify the fungi including gross observation, microscopic examination of tissue squashes, in vitro culture and histopathology. Koch's postulate was fulfilled to confirm the fungal pathogenicity. The challenged fungi altered the hepatorenal indicators (ALP, AST, ALT, urea and creatinine) and oxidative stress biomarkers (MDA, CAT and SOD). The results indicate the virulence of the isolated fungi, and future studies are needed for treatment trials.

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AspergillusDiversity Associated with Fungal Diseases on Fish with Molecular Based

Cited by 6 publications

References 5 publications

Molecular identification and phylogenetic analysis of fungal pathogens isolated from diseased fish in Xinjiang, China

Molecular identification and phylogenetic analysis of fungal pathogens isolated from diseased fish in Xinjiang, China

Incidence and molecular characterization of fungi and yeast isolated from cultured catfish and Nile tilapia.

Detection, diagnosis, Koch’s postulate, hepatorenal and antioxidant indicators for some systemic pathogenic fungi invading the liver and kidneys of African catfish ( Clarias gariepinus ) in Egypt with a histopathological approach

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