<i>Aspergillus niger</i> upregulated glycerolipid metabolism and ethanol utilization pathway under ethanol stress

Vinayavekhin, Nawaporn; Kongchai, Wimonsiri; Piapukiew, Jittra; Chavasiri, Warinthorn

doi:10.1002/mbo3.948

Cited by 6 publications

(5 citation statements)

References 46 publications

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“…Subsequently, hydrophobic metabolites were extracted separately for the intra- (cell pellet) and extracellular (supernatant) components using a 2:1 ( v / v ) mixture of chloroform to methanol. The concentrated extracts were then analyzed using the LC–MS-based untargeted metabolomics platforms [ 25 , 26 ].…”

Section: Resultsmentioning

confidence: 99%

“…Next, the chemical structures of the altered metabolite ions were manually characterized using accurate masses, previously reported retention time [ 25 , 26 ], and tandem mass spectra (S1 Fig in S1 File ). Out of the 111 metabolite ions with changed levels, probable structures could be assigned to 83 ions or 75%, which is relatively high for untargeted metabolomics analyses (S1–S7 Tables in S1 File ).…”

Section: Resultsmentioning

confidence: 99%

“…Metabolites extraction protocols were modified from those described previously [ 25 ]. After incubation with or without acoustic vibration for 48 h, bacterial cultures were vortexed briefly and vigorously to mix, and 700 μ L of cultures were collected for OD 600 measurement.…”

Section: Methodsmentioning

confidence: 99%

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Metabolomics responses and tolerance of Pseudomonas aeruginosa under acoustic vibration stress

Vinayavekhin,

Wattanophas,

Murphy

et al. 2024

PLoS ONE

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Sound has been shown to impact microbial behaviors. However, our understanding of the chemical and molecular mechanisms underlying these microbial responses to acoustic vibration is limited. In this study, we used untargeted metabolomics analysis to investigate the effects of 100-Hz acoustic vibration on the intra- and extracellular hydrophobic metabolites of P. aeruginosa PAO1. Our findings revealed increased levels of fatty acids and their derivatives, quinolones, and N-acylethanolamines upon sound exposure, while rhamnolipids (RLs) showed decreased levels. Further quantitative real-time polymerase chain reaction experiments showed slight downregulation of the rhlA gene (1.3-fold) and upregulation of fabY (1.5-fold), fadE (1.7-fold), and pqsA (1.4-fold) genes, which are associated with RL, fatty acid, and quinolone biosynthesis. However, no alterations in the genes related to the rpoS regulators or quorum-sensing networks were observed. Supplementing sodium oleate to P. aeruginosa cultures to simulate the effects of sound resulted in increased tolerance of P. aeruginosa in the presence of sound at 48 h, suggesting a potential novel response-tolerance correlation. In contrast, adding RL, which went against the response direction, did not affect its growth. Overall, these findings provide potential implications for the control and manipulation of virulence and bacterial characteristics for medical and industrial applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Metabolomics responses and tolerance of Pseudomonas aeruginosa under acoustic vibration stress

Vinayavekhin,

Wattanophas,

Murphy

et al. 2024

PLoS ONE

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“…Aspergillus niger resisted copper stress by converting sorbitol from glucose to produce a large amount of mannitol [ 27 ]. When Aspergillus niger was exposed to 5% ethanol stress, its growth amount was about 70% less than that under normal growth conditions [ 28 ]. Using untargeted metabolomics to study its reaction mechanism, it was found that TAG, DAG, and hTAGs significantly accumulated.…”

Section: Application Of Metabolomics In the Field Of Fungal Researchmentioning

confidence: 99%

Application of Metabolomics in Fungal Research

Jian

Liu

et al. 2022

Molecules

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Metabolomics is an essential method to study the dynamic changes of metabolic networks and products using modern analytical techniques, as well as reveal the life phenomena and their inherent laws. Currently, more and more attention has been paid to the development of metabolic histochemistry in the fungus field. This paper reviews the application of metabolomics in fungal research from five aspects: identification, response to stress, metabolite discovery, metabolism engineering, and fungal interactions with plants.

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“… ( A ) Pathway analysis overview depicting altered metabolic pathways in A. flavus from the 200-μg/mL CV-treated group and the control. ( B ) Biosynthesis of glycerolipids and phospholipids [ 35 ] in A. flavus [ 36 ]. The red boxes showed upregulated lipids of mycelium treated with 200-μg/mL CV vs. the control, the green boxes indicated downregulated lipids and the gray boxes were lipids undetected or not changed significantly in the pathways.…”

Section: Figurementioning

confidence: 99%

UHPLC-HRMS-Based Untargeted Lipidomics Reveal Mechanism of Antifungal Activity of Carvacrol against Aspergillus flavus

Wang

2021

Foods

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Aspergillus flavus is a common contaminant in grain, oil and their products. Its metabolite aflatoxin B1 (AFB1) has been proved to be highly carcinogenic. Therefore, it is of great importance to find possible antifungal substances to inhibit the growth and toxin production of Aspergillus flavus. Carvacrol (CV) was reported as a potent antifungal monoterpene derived from plants. In this paper, the antifungal effects and mechanism of CV on Aspergillus flavus were investigated. CV was shown good inhibition on the growth of Aspergillus flavus and the production of AFB1. CV used in concentrations ranging from 0, 50, 100 and 200 μg/mL inhibited the germination of spores, mycelia growth and AFB1 production dose-dependently. To explore the antifungal mechanism of CV on Aspergillus flavus, we also detected the ergosterol content of Aspergillus flavus mycelia, employed Scanning Electron Microscopy (SEM) to observe mycelia morphology and utilized Ultra-High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry (UHPLC-HRMS) to explore the lipidome profiles of Aspergillus flavus. The results showed that the production of ergosterol of mycelia was reduced as the CV treatment concentration increased. SEM photographs demonstrated a rough surface and a reduction in the thickness of hyphae in Aspergillus flavus treated with CV (200 µg/mL). In positive ion mode, 21 lipids of Aspergillus flavus mycelium were downregulated, and 11 lipids were upregulated after treatment with 200-µg/mL CV. In negative ion mode, nine lipids of Aspergillus flavus mycelium were downregulated, and seven lipids upregulated after treatment with 200-µg/mL CV. In addition, the analysis of different lipid metabolic pathways between the control and 200-µg/mL CV-treated groups demonstrated that glycerophospholipid metabolism was the most enriched pathway related to CV treatment.

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Aspergillus niger upregulated glycerolipid metabolism and ethanol utilization pathway under ethanol stress

Cited by 6 publications

References 46 publications

Metabolomics responses and tolerance of Pseudomonas aeruginosa under acoustic vibration stress

Metabolomics responses and tolerance of Pseudomonas aeruginosa under acoustic vibration stress

Application of Metabolomics in Fungal Research

UHPLC-HRMS-Based Untargeted Lipidomics Reveal Mechanism of Antifungal Activity of Carvacrol against Aspergillus flavus

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