Astragalus membranaceus lectin (AML) induces caspase‐dependent apoptosis in human leukemia cells

Abstract

Our results suggest that AML could act as a potential anti-cancer drug.

Biochemical Characterization of a Novel l -Asparaginase with Low Glutaminase Activity from Rhizomucor miehei and Its Application in Food Safety and Leukemia Treatment

“…Cell cultures were incubated at 37°C in a humidified atmosphere with 5% CO 2 . Cell viability was measured by the MTT method as described previously, with some modifications (16). Briefly, cells were dispensed in 96-well flat-bottom microtiter plates at a density of 1 ϫ 10 5 cells/ml.…”

“…The heterologous expression, purification, and characterization of this recombinant L-asparaginase (RmAsnase) are described. We further investigate the application of RmAsnase for the degradation of acrylamide in baked goods and for synergistic treatment of leukemia when combined with Astragalus membranaceus lectin (AML), reported in our previous work (16).…”

Abrus agglutinin suppresses human hepatocellular carcinoma in vitro and in vivo by inducing caspase-mediated cell death

“…Additionally, ConA has been reported to elicit autophagic cell death in HCC [31] . In addition to this study, we have shown naceus that induces caspase-dependent apoptosis in human leukemia cells [30] . The immunoblot analysis of AGG in our study revealed a sharp upregulation of Bax and a simultaneous downregulation of Bcl-2, along with PARP cleavage, in a dose-oriented manner.…”

“…In this regard, several plant lectins, including Phaseolus vulgaris lectin, Concanavalin-A (ConA) and lectins from Astragalus membranaceus and Momordica charantia, induce apoptotic cell death in cancer cells that originate from different sources and tissues [26][27][28][29][30] . Additionally, ConA has been reported to elicit autophagic cell death in HCC [31] .…”

Biological Applications of Plants and Algae Lectins: An Overview

“…An interesting study conducted by [39] showed the pro-apoptotic caspase-dependent activity of the lectin isolated from Astragalus membranaceus in leukemia cell line (CML K562). These results showed a close relationship with the low expression of BCL-2 (anti-apoptotic protein) indicating that the lectin is active through the mitochondrial apoptotic pathway [40].…”