<i>AtBXL1</i>, a novel higher plant (<i>Arabidopsis thaliana</i>) putative beta‐xylosidase gene, is involved in secondary cell wall metabolism and plant development

Goujon, Thomas; Minić, Zoran; Amrani, Abdelhak El; Lerouxel, Olivier; Aletti, Estelle; Lapierre, Catherine; Joseleau, J.P.; Jouanin, Lise

doi:10.1046/j.1365-313x.2003.01654.x

Cited by 127 publications

(130 citation statements)

References 74 publications

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“…In Nepenthes, the GH family 18 enzyme is encoded by a single-copy gene that is up-regulated in response to prey in both the pitted glands and surrounding tissues (54). Galactosidases and xylosidases (55) are also among the genes with the hydrolase annotation, and enzymes encoding both have been identified in the Nepenthes trap fluid proteome (56,57). Nepenthes and Drosera (carnivorous sundew plant) digestive mucilage contains galactose and xylose (58), which may require breakdown for peptide and other nutrient absorption in U. gibba traps as well (59).…”

Section: Significancementioning

confidence: 99%

Long-read sequencing uncovers the adaptive topography of a carnivorous plant genome

Lan

Renner

Ibarra-Laclette

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Utricularia gibba, the humped bladderwort, is a carnivorous plant that retains a tiny nuclear genome despite at least two rounds of whole genome duplication (WGD) since common ancestry with grapevine and other species. We used a third-generation genome assembly with several complete chromosomes to reconstruct the two most recent lineage-specific ancestral genomes that led to the modern U. gibba genome structure. Patterns of subgenome dominance in the most recent WGD, both architectural and transcriptional, are suggestive of allopolyploidization, which may have generated genomic novelty and led to instantaneous speciation. Syntenic duplicates retained in polyploid blocks are enriched for transcription factor functions, whereas gene copies derived from ongoing tandem duplication events are enriched in metabolic functions potentially important for a carnivorous plant. Among these are tandem arrays of cysteine protease genes with trap-specific expression that evolved within a protein family known to be useful in the digestion of animal prey. Further enriched functions among tandem duplicates (also with trap-enhanced expression) include peptide transport (intercellular movement of broken-down prey proteins), ATPase activities (bladder-trap acidification and transmembrane nutrient transport), hydrolase and chitinase activities (breakdown of prey polysaccharides), and cell-wall dynamic components possibly associated with active bladder movements. Whereas independently polyploid Arabidopsis syntenic gene duplicates are similarly enriched for transcriptional regulatory activities, Arabidopsis tandems are distinct from those of U. gibba, while still metabolic and likely reflecting unique adaptations of that species. Taken together, these findings highlight the special importance of tandem duplications in the adaptive landscapes of a carnivorous plant genome.

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Section: Significancementioning

confidence: 99%

Long-read sequencing uncovers the adaptive topography of a carnivorous plant genome

Lan

Renner

Ibarra-Laclette

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…β-d-xylosidase gene (AtBXL1) has been reported to be involved in secondary cell wall xylan synthesis (Goujon et al 2003). Although LOC_Os11g19210 does not have higher sequence similarity to AtBXL1, it is possible that LOC_Os11g19210 is involved in secondary cell wall synthesis in rice because this gene is highly expressed in the whole internode.…”

Section: Genes Differentially Expressed In the Wild-type Whole Internodementioning

confidence: 99%

Transcription profiling identifies candidate genes for secondary cell wall formation and hydroxycinnamoyl-arabinoxylan biosynthesis in the rice internode

Nakano

Nishikubo

Sato-Izawa

et al. 2013

Plant Biotechnology

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Microarray analysis was used to identify candidate genes that are involved in the formation of secondary cell walls and hydroxycinnamoyl-arabinoxylan (AX) in rice. In order to identify genes involved in secondary cell wall formation, gene expression was compared between wild-type whole internodes that contain cells with thickened secondary cell walls, such as vascular and cortical fiber cells, and wild-type internode parenchyma cells without secondary cell walls. In addition, gene expression was compared between the internode parenchyma of Fukei71 (F71), a rice dwarf mutant that accumulates large amounts of hydroxycinnamoyl-AX in pith parenchyma cells, and wild-type pith parenchyma cells to identify hydroxycinnamoyl-AX-related genes. To address the significant expression of candidate genes, gene lists were prepared for the phenylpropanoid pathway, major carbon metabolism, and cell wall biosynthesis, which is a useful platform to analyze cell wall formation in rice. The data indicated that a number of rice genes are potentially associated with secondary cell wall formation, such as the up-regulation of genes encoding cellulose synthase subunit A and ferulate 5-hydroxylase in wild-type whole internodes. Similarly, for hydroxycinnamoyl-AX synthesis, the expression of several genes changed, such as the downregulation of genes encoding cinnamoyl-CoA reductase and the up-regulation of the phosphoenolpyruvate carboxykinase gene in F71 pith parenchyma.

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“…Soluble phenolics from 15-d-old plate-grown seedlings were analyzed as described by Goujon et al (2003). Briefly, phenolics from whole control (Ws) and homozygous or heterozygous cyp98A3 and control plants (six plants per assay carefully weighted and three assays per line) were extracted by a prolonged maceration in 2 mL of methanol:water (4:1; v/v) mixture in the presence of morin hydrate (5 mg; Fluka) as internal standard.…”

Section: Lc-ms Analyses Of Soluble Phenolicsmentioning

confidence: 99%

A coumaroyl-ester-3-hydroxylase Insertion Mutant Reveals the Existence of Nonredundant meta-Hydroxylation Pathways and Essential Roles for Phenolic Precursors in Cell Expansion and Plant Growth

et al. 2005

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Cytochromes P450 monooxygenases from the CYP98 family catalyze the meta-hydroxylation step in the phenylpropanoid biosynthetic pathway. The ref8 Arabidopsis (Arabidopsis thaliana) mutant, with a point mutation in the CYP98A3 gene, was previously described to show developmental defects, changes in lignin composition, and lack of soluble sinapoyl esters. We isolated a T-DNA insertion mutant in CYP98A3 and show that this mutation leads to a more drastic inhibition of plant development and inhibition of cell growth. Similar to the ref8 mutant, the insertion mutant has reduced lignin content, with stem lignin essentially made of p-hydroxyphenyl units and trace amounts of guaiacyl and syringyl units. However, its roots display an ectopic lignification and a substantial proportion of guaiacyl and syringyl units, suggesting the occurrence of an alternative CYP98A3-independent meta-hydroxylation mechanism active mainly in the roots. Relative to the control, mutant plantlets produce very low amounts of sinapoyl esters, but accumulate flavonol glycosides. Reduced cell growth seems correlated with alterations in the abundance of cell wall polysaccharides, in particular decrease in crystalline cellulose, and profound modifications in gene expression and homeostasis reminiscent of a stress response. CYP98A3 thus constitutes a critical bottleneck in the phenylpropanoid pathway and in the synthesis of compounds controlling plant development. CYP98A3 cosuppressed lines show a gradation of developmental defects and changes in lignin content (40% reduction) and structure (prominent frequency of p-hydroxyphenyl units), but content in foliar sinapoyl esters is similar to the control. The purple coloration of their leaves is correlated to the accumulation of sinapoylated anthocyanins.

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AtBXL1, a novel higher plant (Arabidopsis thaliana) putative beta‐xylosidase gene, is involved in secondary cell wall metabolism and plant development

Cited by 127 publications

References 74 publications

Long-read sequencing uncovers the adaptive topography of a carnivorous plant genome

Long-read sequencing uncovers the adaptive topography of a carnivorous plant genome

Transcription profiling identifies candidate genes for secondary cell wall formation and hydroxycinnamoyl-arabinoxylan biosynthesis in the rice internode

A coumaroyl-ester-3-hydroxylase Insertion Mutant Reveals the Existence of Nonredundant meta-Hydroxylation Pathways and Essential Roles for Phenolic Precursors in Cell Expansion and Plant Growth

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