AtDMC1, the Arabidopsis homologue of the yeast DMC1 gene: characterization, transposon‐induced allelic variation and meiosis‐associated expression

Klimyuk, Victor; Jones, Jonathan D. G.

doi:10.1046/j.1365-313x.1997.11010001.x

Cited by 214 publications

(208 citation statements)

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“…To investigate the basis of this observation, immunolocalization studies were conducted with Abs that recognize key recombination proteins. Localization of the Arabidopsis RecA homologs AtDMC1 and AtRAD51 (Klimyuk and Jones 1997;Li et al 2004) was conducted in chromosome preparations from wildtype and asy1 meiocytes following BrdU pulse-labeling. In wild-type nuclei at 5 h post-S phase there was no evidence of AtDMC1 localization, but by 12 h there was significant accumulation of foci to >50 foci per meiocyte.…”

Section: Asy1 Is Required For Normal Meiotic Recombination Progressionmentioning

confidence: 99%

ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis

Sanchez‐Moran¹,

Santos²,

Jones³

et al. 2007

Genes Dev.

250

326

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ASY1 is an Arabidopsis protein required for synapsis and crossover formation during meiosis. The chronology of meiotic recombination has been investigated in wild type and an asy1 mutant. We observe a delay between the appearance of chromatin-associated AtSPO11-1 foci and DNA double-strand break (DSB) formation, which occurs contemporaneously with chromosome axis formation and transition of ASY1 from chromatin-associated foci to a linear axis-associated signal. DSBs are formed independently of ASY1 in an AtSPO11-1-dependent manner. They are partially restored in Atspo11-1-3 using cisplatin, but their control appears abnormal. Axis morphogenesis is independent of ASY1, but axis structure may be compromised in asy1. Localization of the strand exchange proteins AtRAD51 and AtDMC1 to the chromatin occurs asynchronously shortly after DSB formation, with AtDMC1 localizing in advance of AtRAD51. In wild-type nuclei, both recombinases form numerous foci that persist for ∼12 h before gradually decreasing in number. In asy1, initial localization of AtDMC1 is normal, but declines abruptly such that interhomolog recombination is severely compromised. Limited ASY1-independent, DMC1-dependent interhomolog recombination remains, but appears restricted to subtelomeric sequences where the homologs are fortuitously in proximity. Thus, ASY1 plays a key role in coordinating the activity of the RecA homologs to create a bias in favor of interhomolog recombination.

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Section: Asy1 Is Required For Normal Meiotic Recombination Progressionmentioning

confidence: 99%

ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis

Sanchez‐Moran¹,

Santos²,

Jones³

et al. 2007

Genes Dev.

250

326

View full text Add to dashboard Cite

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“…An 11-kb DNA fragment covering the entire Arabidopsis (ecotype Columbia) genomic DMC1 region (i.e., a HpaI-SalI fragment; see Figure 1A) was introduced into the T-DNA of an Agrobacterium binary vector. In addition to the coding region, this fragment includes the complete AtDMC1 promoter, as defined by Klimyuk and Jones (1997), which could be of functional significance for the precise timing of DMC1 expression. One heterozygous gentamycin-resistant transformant (T 1 ) was recovered among the seeds of the infiltrated plants, and its T 2 progeny was analyzed.…”

Section: Complementation Of the Dmc1 Insertional Mutationmentioning

confidence: 99%

“…Finally, homozygous disruption of the DMC1 gene in mouse parallels some aspect of the yeast dmc1 mutations in that it also affects meiosis, although leading to apoptosis, which was not observed in yeast (Pittman et al, 1998;Yoshida et al, 1998). In Arabidopsis, an ortholog of the yeast DMC1 gene, AtDMC1 , has been described (Sato et al, 1995;Klimyuk and Jones, 1997;Doutriaux et al, 1998). It is a single-copy gene that is expressed in young flower buds when cells are undergoing meiosis.…”

Section: Introductionmentioning

confidence: 99%

“…It is a single-copy gene that is expressed in young flower buds when cells are undergoing meiosis. To the difference of yeast and mouse, AtDMC1 also is found expressed in vegetative cells, at low level in leaves but to a high level in exponentially growing cells from a suspension culture (Klimyuk and Jones, 1997;Doutriaux et al, 1998). However, functional homology between the yeast, mouse, and Arabidopsis DMC1 genes remains to be proven.…”

Section: Introductionmentioning

confidence: 99%

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Random Chromosome Segregation without Meiotic Arrest in Both Male and Female Meiocytes of a dmc1 Mutant of Arabidopsis

et al. 1999

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In yeast, the DMC1 gene is required for interhomolog recombination, which is an essential step for bivalent formation and the correct partition of chromosomes during meiosis I. By using a reverse genetics approach, we were able to identify a T-DNA insertion in AtDMC1 , the Arabidopsis homolog of DMC1 . Homozygotes for the AtDMC1 insertion failed to express AtDMC1 , and their residual fertility was 1.5% that of the wild type. Complete fertility was restored in mutant plants when a wild-type copy of the AtDMC1 gene was reintroduced. Cytogenetical analysis points to a correlation of the sterility phenotype with severely disturbed chromosome behavior during both male and female meiosis. In this study, our data demonstrate that AtDMC1 function is crucial for meiosis in Arabidopsis. However, meiosis can be completed in the Arabidopsis dmc1 mutant, which is not the case for mouse or some yeast mutants.

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“…Phylogenetic analysis indicates that DMC1 is a RecA-like protein [60]. DMC1 homologs have been identified in a wide range of organisms, including budding and fission yeast, mammals, plants, and fungi [61][62][63][64][65][66][67][68][69][70]. In the budding yeast, the severity of dmc1 mutants varies among different strains.…”

Section: Dsb Processing and Function Of Reca Proteinsmentioning

confidence: 99%

Double-stranded DNA breaks and gene functions in recombination and meiosis

Mā

2006

Cell Res

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Gene functions in recombination and meiosis 402 npg REVIEW Meiotic prophase I is a long and complex phase. Homologous recombination is an important process that occurs between homologous chromosomes during meiotic prophase I. Formation of chiasmata, which hold homologous chromosomes together until the metaphase I to anaphase I transition, is critical for proper chromosome segregation. Recent studies have suggested that the SPO11 proteins have conserved functions in a number of organisms in generating sites of double-stranded DNA breaks (DSBs) that are thought to be the starting points of homologous recombination. Processing of these sites of DSBs requires the function of RecA homologs, such as RAD51, DMC1, and others, as suggested by mutant studies; thus the failure to repair these meiotic DSBs results in abnormal chromosomal alternations, leading to disrupted meiosis. Recent discoveries on the functions of these RecA homologs have improved the understanding of the mechanisms underlying meiotic homologous recombination.Cell Research (2006) Meiosis is a highly coordinated cell division, which generates four haploid reproductive cells required for sexual reproduction. Meiosis involves one round of DNA replication and two nuclear divisions, meiosis I and meiosis II. The first nuclear division leads to the segregation of homologous chromosomes (homologs), and is unique to meiosis. During the second division, sister chromatids are separated, resulting in the formation of four haploid nuclei and followed by the meiotic cytokinesis that forms four haploid cells. Similar to mitosis, both meiosis I and meiosis II can be divided into four stages: prophase, metaphase, anaphase, and telophase.However, meiosis I differs from meiosis II as meiosis I involves homology search, pairing, interhomolog recombination, and synapsis of homologs, processes that are required for correct association and segregation of homologs. The prophase stage of meiosis I, or prophase I, has been the target of interest because of the occurrence of these major processes of chromosome interactions. During early prophase I, arms of sister chromosomes are closely associated by protein complexes called cohesins, which are removed during the metaphase I/anaphase I transition. However, the centromere regions remained associated until the segregation of sister chromatids during meiosis II. Homologous pairing is the result of interaction between homologous chromosomes relying on the homology of DNA sequences [1,2], and is considered to be a transient and non-stable association between homologous chromosomes. Pairing between homologous chromosomes facilitates the process of recombination. The recombination process confers the interexchange of genetic information between non-sister chromatids, and generates chiasmata, which ensure proper association between homologous chromosomes prior to chromosome segregation at anaphase I. Synapsis, however, is a stable association by forming synaptonemal complexes between chromosomes. Synaptonemal complexes are threeparti...

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AtDMC1, the Arabidopsis homologue of the yeast DMC1 gene: characterization, transposon‐induced allelic variation and meiosis‐associated expression

Abstract: SummaryBased on homologies between the yeast DMCl and the lily LIM15 meiosis-specific genes, degenerate PCR primers were designed that amplified the Arabidopsis DMCl gene

Cited by 214 publications

References 47 publications

ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis

ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in Arabidopsis

Random Chromosome Segregation without Meiotic Arrest in Both Male and Female Meiocytes of a dmc1 Mutant of Arabidopsis

Double-stranded DNA breaks and gene functions in recombination and meiosis

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