<i>AtSUC3</i>, a gene encoding a new <i>Arabidopsis</i> sucrose transporter, is expressed in cells adjacent to the vascular tissue and in a carpel cell layer

Meyer, Stefan; Melzer, Michael; Truernit, Elisabeth; Hümmer, Carola; Besenbeck, Rainer; Stadler, Ruth; Sauer, Norbert

doi:10.1111/j.1365-313x.2000.00934.x

Cited by 53 publications

(43 citation statements)

References 61 publications

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“…However, subsequent studies have questioned this hypothesis by finding that null mutations in AtSUC3 have no obvious phenotype and that AtSUC3 protein has measurable biochemical activity (Meyer et al, 2000;Barth et al, 2003). Interestingly, the gene is expressed in many sink tissues and is strongly induced by wounding (Meyer et al, 2000(Meyer et al, , 2004. The only report characterizing a group 3 SUT in grasses showed that the rice OsSUT4 gene is expressed in all tissues examined, although at higher levels in sink leaves (Aoki et al, 2003).…”

Section: Group 3 4 and 5 Sut Family Membersmentioning

confidence: 98%

“…Based on sequence characteristics and an initial report of a lack of transport activity, it was postulated that AtSUT2/AtSUC3 might function as a Suc sensor . However, subsequent studies have questioned this hypothesis by finding that null mutations in AtSUC3 have no obvious phenotype and that AtSUC3 protein has measurable biochemical activity (Meyer et al, 2000;Barth et al, 2003). Interestingly, the gene is expressed in many sink tissues and is strongly induced by wounding (Meyer et al, 2000(Meyer et al, , 2004.…”

Section: Group 3 4 and 5 Sut Family Membersmentioning

confidence: 99%

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Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading

2009

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Section: Group 3 4 and 5 Sut Family Membersmentioning

confidence: 98%

Section: Group 3 4 and 5 Sut Family Membersmentioning

confidence: 99%

Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading

2009

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“…SUC1 expression is highly dynamic at the transcriptional level (Vaughn et al, 2002;Li et al, 2003;Sivitz et al, 2008;Zhou et al, 2009); thus, regulation of SUC1 expression might represent a target for ethylene. Transcript levels were examined for four SUC genes, SUC2, -3, and -4 in addition to SUC1, which are expressed at the seedling stage, as well as in the adult leaves (Gottwald et al, 2000;Meyer et al, 2000;Weise et al, 2000), where anthocyanin accumulation is observed (Supplemental Fig. S2A).…”

Section: Transcript Levels Of Suc Transporters In Col0 and Ethylene Amentioning

confidence: 99%

“…SUC1 is expressed in roots, pollen, and trichomes (Sivitz et al, 2008). SUC2 and SUC4 are thought to be involved in Suc loading in companion cells (Gottwald et al, 2000) and minor veins (Meyer et al, 2000;Weise et al, 2000), respectively. SUC3 (SUT2) has been characterized as a weak low-affinity transporter .…”

mentioning

confidence: 99%

Ethylene Suppression of Sugar-Induced Anthocyanin Pigmentation in Arabidopsis

et al. 2010

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Anthocyanin accumulation is regulated negatively by ethylene signaling and positively by sugar and light signaling. However, the antagonistic interactions underlying these signalings remain to be elucidated fully. We show that ethylene inhibits anthocyanin accumulation induced by sucrose (Suc) and light by suppressing the expression of transcription factors that positively regulate anthocyanin biosynthesis, including GLABRA3, TRANSPARENT TESTA8, and PRODUCTION OF AN-THOCYANIN PIGMENT1, while stimulating the concomitant expression of the negative R3-MYB regulator MYBL2. Genetic analyses show that the ethylene-mediated suppression of anthocyanin accumulation is dependent upon ethylene signaling components responsible for the triple response. Furthermore, these positive and negative signaling pathways appear to be under photosynthetic control. Suc and light induction of anthocyanin accumulation was almost fully inhibited in wild-type Arabidopsis (Arabidopsis thaliana) ecotype Columbia and ethylene (ethylene response1 [etr1-1]) and light (long hypocotyl1 [hy1], cryptochrome1/2, and hy5) signaling mutants treated with the photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea. The transcript level of the sugar transporter gene SUC1 was enhanced in ecotype Columbia treated with the ethylene-binding inhibitor silver and in etr1-1, ethylene insensitive2 (ein2-1), and ein3 ein3-like1 mutants. In contrast, 3-(3,4-dichlorophenyl)-1,1-dimethylurea treatment reduced SUC1 expression, which indicates strongly that SUC1 represents an integrator for signals provided by sugar, light, and ethylene. SUC1 mutations lowered accumulations of anthocyanin pigment, soluble sugar content, and ethylene production in response to Suc and light signals. These data demonstrate that the suppression of SUC1 expression by ethylene inhibits Suc-induced anthocyanin accumulation in the presence of light and, hence, fine-tunes anthocyanin homeostasis.Anthocyanins play key roles in many plant physiological processes; for instance, they form photoprotective screens in vegetative tissue, act as visual attractors to aid pollination and seed dispersal, and function as antimicrobial agents and feeding deterrents in the defense response (Winkel-Shirley, 2001;Steyn et al., 2002). The anthocyanin biosynthetic pathway is well described in plants. In Arabidopsis (Arabidopsis thaliana) and other plants, including Antirrhinum majus (snapdragon) and Petunia hybrida (petunia), early biosynthesis genes (EBGs) such as chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), and flavonoid 3#-hydroxylase (F3#H), which are common to different flavonoid subpathways, are induced prior to late biosynthesis genes (LBGs) such as dihydroflavonol 4-reductase (DFR), leucoanthocyanidin oxygenase (LDOX), anthocyanidin reductase (ANR), and UDP-glucose:flavonoid 3-O-glucosyltransferase (UF3GT; Pelletier et al., 1997). EBGs and LBGs are transcriptionally activated by R2R3-MYBs, such as PRODUCTION OF ANTHOCYANIN PIG-MENT1 (PAP1) a...

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“…PCR amplification was carried out with 35 cycles of 2 min at 94 °C, 10 s at 98 °C, 30 s at 56 °C, and 45 s at 68 °C with KOD-Plus-Neo (TOYOBO, Japan). The PCR product was double digested using EcoR I and Sal I and subsequently subcloned into the Saccharomyces cerevisiae/Escherichia coli shuttle vector pEX-Tag (Meyer et al, 2000), which was also doubledigested by EcoR I and Sal I. The recombinant plasmid was transformed into the E. coli strain and sequenced by the Sanger method.…”

Section: Sequence Cloning and Nucleotide Polymorphism Analysesmentioning

confidence: 99%

Cloning and characterization of a serine/threonine protein phosphatase2A-encoding gene IbPP2A1 from Ipomoea batatas (L.) Lam.

Xu¹,

Yong²,

Shao³

et al. 2017

Turk J Biol

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Sweet potato is one of the most important food crops with strong environmental adaptability. Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that plays crucial roles in hormone signal transduction and abiotic stress response. Characterization of PP2A can elucidate the mechanisms of stress resistance in this crop. In this study, a total of 15 PP2A transcripts, including nine regulatory subunit-encoding sequences and six catalytic subunit-encoding sequences, were identified from sweet potato and found to be expressed at varying levels. Only one contained a complete open reading frame and encoded a B regulatory subunit (termed IbPP2A1). Ten polymorphic sites were distributed in the coding region of this gene, but most did not result in amino acid change. RNA sequencing-based digital gene expression profiling showed that this PP2A gene was primarily expressed in fibrous roots and developing tuberous roots under natural conditions. After drought, salinity, and alkaline stress treatment, the expression of IbPP2A1 was obviously upregulated in stems and tuberous roots at 2 days, whereas the expression of IbPP2A1 in leaves was only upregulated by drought stress at 2 days. However, heterogeneous expression of IbPP2A1 did not enhance abiotic stress tolerance in recombinant Saccharomyces cerevisiae. The results described here indicate that IbPP2A1 is an abiotic stress-responsive gene, but it could not work alone in vitro. This study provides a preliminary but global insight into PP2A proteins in sweet potato for further investigations on improving stress tolerance in this crop.

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AtSUC3, a gene encoding a new Arabidopsis sucrose transporter, is expressed in cells adjacent to the vascular tissue and in a carpel cell layer

Cited by 53 publications

References 61 publications

Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading

Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading

Ethylene Suppression of Sugar-Induced Anthocyanin Pigmentation in Arabidopsis

Cloning and characterization of a serine/threonine protein phosphatase2A-encoding gene IbPP2A1 from Ipomoea batatas (L.) Lam.

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