<i>Bacillus cereus</i>
            iron uptake protein fishes out an unstable ferric citrate trimer

Fukushima, Tatsuya; Sia, Allyson K.; Allred, Benjamin E.; Nichiporuk, Rita V.; Zhou, Zhongrui; Andersen, Ulla N.; Raymond, Kenneth N.

doi:10.1073/pnas.1210131109

Cited by 31 publications

(43 citation statements)

References 32 publications

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“…A recent study on Fe uptake in Bacillus cereus demonstrated for the first time that a protein, FctC, could act as a specific binder for the Fe 3 cit 3 complex. 27 Even if the structure of Al 3 cit 3 seems to be slightly different from the one of Fe 3 cit 3 , 27 it could be hypothesized that similar proteins could occur in higher organisms such as plants and for other metal complexes such as Al 3 cit 3 .…”

Section: Al and Fe Are Complexed By Tricitratementioning

confidence: 99%

“…16 The complex aqueous coordination chemistry of Al, the lack of a suitable multiisotopic profile and the typical instability of metal complexes that renders concentration procedures unfeasible have slowed the understanding of the physiology of Al detoxification in tolerant plants. Most studies have been based on 27 Al NMR spectroscopy with a consequence that only plants accumulating extremely high concentrations could be examined. A 1 : 1 complex of Al and citrate has been detected in the xylem of several Al accumulating plants by 27 Al NMR spectroscopy.…”

Section: Identification Of a Tri-al Tricitrate Complex Following A Mamentioning

confidence: 99%

“…Most studies have been based on 27 Al NMR spectroscopy with a consequence that only plants accumulating extremely high concentrations could be examined. A 1 : 1 complex of Al and citrate has been detected in the xylem of several Al accumulating plants by 27 Al NMR spectroscopy. 17 ESI-MS experiments confirmed that in Hydrangea macrophylla cell sap an Al-citrate complex (m/z 215) occurs.…”

Section: Identification Of a Tri-al Tricitrate Complex Following A Mamentioning

confidence: 99%

“…The fact that, of the biologically relevant elements, Fe has the closest ionic radius to the one of Al might explain their binding by the same ligand considering that the ionic radius is one of the most important predictive parameters relating to the biological behavior of metal ions. 25 The relative concentration ratios between the Al-and Fe-complex determined using the relative 27 Al and 56 Fe signal intensities (depicted in Fig. 1C) combined with the sensitivity of the instrument for these isotopes and the fact that the estimated free activity of Fe in the culture medium supplemented with 400 mM is higher than that of Al (Fig.…”

Section: Al and Fe Are Complexed By Tricitratementioning

confidence: 99%

“…Even if, to date, 27 Al nuclear magnetic resonance (NMR) spectroscopy has been used almost exclusively to study the speciation of Al in plants, as it can provide structural information about Al-complexes directly from intact samples, the inherent low sensitivity and low discriminating capacity of NMR spectroscopy for Al is a drawback that has prevented accurate speciation. 7 Mass spectrometry using electrospray ionization (ESI-MS) is a technique that offers increased sensitivity and great potential for identification of metal complexes but has found little application in the field of Al-speciation due to the lack of a suitable isotope.…”

Section: Introductionmentioning

confidence: 99%

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Identification of the tri-Al tricitrate complex in Plantago almogravensis by hydrophilic interaction LC with parallel ICP-MS and electrospray Orbitrap MS/MS detection

et al. 2013

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The identification of the ligands binding Al is essential to understand the mechanisms by which plants detoxify Al internally. However, studies concerning the speciation of Al have been frustrated by its complex chemistry. This work describes the identification of the tri-Al tricitrate (Al 3 cit 3 ) complex in Plantago almogravensis, encompassing an integrated mass spectrometry approach based on hydrophilic interaction liquid chromatography (HILIC) and parallel detection by ICP-MS and ESI-MS/MS. This work also reports that both Al and Fe are bound by tricitrate, sometimes simultaneously, and the consequences of this finding are discussed. Of the complexes separated by size exclusion chromatography, Al 3 cit 3 is the most stable occurring in P. almogravensis as it was the only one recovered after HILIC. This approach provided new information on the mechanism of Al detoxification in P. almogravensis, namely that Al is bound by the organic acid citrate and that the relative concentration of the detected complexes is affected by the organ type and internal Al concentration, and has potential for studying the speciation of Al in less tolerant plants.

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Section: Al and Fe Are Complexed By Tricitratementioning

confidence: 99%