<i>Bacillus subtilis</i> as a host for natural product discovery and engineering of biosynthetic gene clusters

Put, Hanne; Gerstmans, Hans; Vande Capelle, Hanne; Fauvart, Maarten; Michiels, Jan; Masschelein, Joleen

doi:10.1039/d3np00065f

Nat. Prod. Rep.

2024

DOI: 10.1039/d3np00065f

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Bacillus subtilis as a host for natural product discovery and engineering of biosynthetic gene clusters

Hanne Put,

Hans Gerstmans,

Hanne Vande Capelle

et al.

Abstract: This review explores the use of Bacillus as a heterologous host for natural product discovery and engineering, highlighting its unique traits, successful natural product examples and available genetic engineering tools.

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Cited by 5 publications

References 315 publications

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Bacillus velezensis: a versatile ally in the battle against phytopathogens—insights and prospects

Kenfaoui,

Dutilloy,

Benchlih

et al. 2024

Appl Microbiol Biotechnol

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Bacillus velezensis: a versatile ally in the battle against phytopathogens—insights and prospects

Kenfaoui,

Dutilloy,

Benchlih

et al. 2024

Appl Microbiol Biotechnol

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Advances of microbial xylanases in the application of flour industries: A comprehensive review

Liu,

Zhang,

et al. 2024

International Journal of Biological Macromolecules

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Characterization of Lomofungin Gene Cluster Enables the Biosynthesis of Related Phenazine Derivatives

Deng,

Li,

Sheng

et al. 2024

ACS Synth. Biol.

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Phenazine-based small molecules are nitrogen-containing heterocyclic compounds with diverse bioactivities and electron transfer properties that exhibit promising applications in pharmaceutical and electrochemical industries. However, the biosynthetic mechanism of highly substituted natural phenazines remains poorly understood. In this study, we report the direct cloning and heterologous expression of the lomofungin biosynthetic gene cluster (BGC) from Streptomyces lomondensis S015. Reconstruction and overexpression of the BGCs in Streptomyces coelicolor M1152 resulted in eight phenazine derivatives including two novel hybrid phenazine metabolites, and the biosynthetic pathway of lomofungin was proposed. Furthermore, gene deletion suggested that NAD(P)H-dependent oxidoreductase gene lomo14 is a nonessential gene in the biosynthesis of lomofungin. Cytotoxicity evaluation of the isolated phenazines and lomofungin was performed. Specifically, lomofungin shows substantial inhibition against two human cancer cells, HCT116 and 5637. These results provide insights into the biosynthetic mechanism of lomofungin, which will be useful for the directed biosynthesis of natural phenazine derivatives.

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Bacillus subtilis as a host for natural product discovery and engineering of biosynthetic gene clusters

Abstract: This review explores the use of Bacillus as a heterologous host for natural product discovery and engineering, highlighting its unique traits, successful natural product examples and available genetic engineering tools.

Cited by 5 publications

References 315 publications

Bacillus velezensis: a versatile ally in the battle against phytopathogens—insights and prospects

Bacillus velezensis: a versatile ally in the battle against phytopathogens—insights and prospects

Advances of microbial xylanases in the application of flour industries: A comprehensive review

Characterization of Lomofungin Gene Cluster Enables the Biosynthesis of Related Phenazine Derivatives

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