Bacillus tianmuensissp. nov., isolated from soil in Tianmu Mountain national natural reserve, Hangzhou, China

Abstract

In a project aiming to isolate strains with the ability to produce nonribosomal peptides, a gram-negative, endospore-forming, rod-shaped strain, designated B5(T), was isolated from a soil sample collected from Tianmu Mountain national natural reserve in Hangzhou, China. Strain B5(T) contained meso-diaminopimelic acid in the cell wall peptidoglycan. The major cellular fatty acids were anteiso-C(15:0) and iso-C(15:0). The DNA G+C content was 42.5 mol%. The phylogenetic analysis based on 16S rRNA gene sequence indicated that strain B5(T) fell within the genus Bacillus, with highest sequence similarity values to Bacillus barbaricus DSM 14730(T) (96.4%) and Bacillus macauensis JCM 13285(T) (95.5%). The isolate, however, could be distinguished from Bacillus strains with validly published names by low 16S rRNA gene sequence similarity values, distinct phenotypic and chemotaxonomic characteristics. On the basis of these polyphasic evidences, it is demonstrated that the isolate B5(T) represents a novel species of the genus Bacillus, for which the name Bacillus tianmuensis sp. nov. is proposed. The type strain is B5(T) (=DSM 22111(T)=CGMCC 1.8879(T)).

Isolation and partial characterization of antibiotics produced by Paenibacillus elgii B69

“…The 16S rRNA gene was amplified using two universal primers as described by Wu et al (2007). PCR amplification and DNA sequencing were conducted using the method of Wen et al (2009). The 16S rRNA gene sequence obtained was compared with the corresponding reference sequences retrieved from GenBank databases by blast search.…”

Microdiversity of Deep-Sea <i>Bacillales</i> Isolated from Tyrrhenian Sea Sediments as Revealed by ARISA, 16S rRNA Gene Sequencing and BOX-PCR Fingerprinting

“…The remaining isolates of the genus Bacillus were isolated from the superficial horizon of seamount station 6 and were respectively assigned to B. tianmuensis (CS 6067) (54) and B. barbaricus / B. arsenicus complex (CS 6054, 603, 6060, 6055 and CS 6052). These two species were characterized by their resistance to a high concentration of arsenic (45).…”

“…These two species were characterized by their resistance to a high concentration of arsenic (45). Wen et al (54) demonstrated that, within the genus Bacillus , the highest 16S rRNA sequence similarity was found between B. barbaricus and B. tianmuensis . In our study, the high phylogenetic relationship between these two species was confirmed by their segregation into the same cluster (Fig.…”