Bacopa monniera extract Induces apoptosis in murine sarcoma cells (S‐180)

Abstract

The Ayurvedic system of medicine recommends Bacopa monniera (BM) in the treatment of tumors. The present study aims to determine the mode of cell death induced by the ethanolic extract of BM in mouse S-180 cells. BM-treated S-180 cells were assessed for cell viability in a dose- and time-dependent manner using dye exclusion studies. Morphological changes in the BM-treated and untreated cells were studied by transmission electron microscopy (TEM). Glutathione (GSH) levels were quantified and the percentage of apoptotic cells was determined using Annexin V-FITC assay. The results indicate that BM induces a dose- and time-dependent loss of cell viability with maximum cytotoxicity at 48 h at a concentration of 550 microg/ml. TEM studies indicate apoptosis in the BM-treated cells. GSH levels were decreased in the BM-treated cells and Annexin V-FITC assay revealed 90.2% of the cells as apoptotic. Conclusively, BM induces cell death by apoptosis in S-180 cells.

Betulinic acid inhibits endotoxin‐stimulated phosphorylation cascade and pro‐inflammatory prostaglandin E2 production in human peripheral blood mononuclear cells

“…A number of studies have suggested that certain bioactive chemicals present in plants may protect against inflammation (Plummer et al ., 1999; Murakami and Ohigashi, 2007). Bacopa monniera (L.) Wettst (family: Scrophulariaceae family) is a renowned Ayurvedic plant reported to possess memory‐enhancing (Mukherjee and Dey, 1996), cognitive (Vohora et al ., 2000), antioxidant (Chowdhuri et al ., 2002), anti‐fertility (Singh and Singh, 2009), hepatoprotective (Vijayan and Helen, 2007), anti‐cancer (Rohini and Devi, 2008), anti‐ulcer (Sairam et al ., 2001) and anti‐inflammatory (Viji and Helen, 2008) properties. The plant is enriched with many phytochemicals of which betulinic acid (BA), a triterpenoid belonging to lupane series, was recently explored to its topoisomerase inhibitory potential (Chowdhury et al ., 2002).…”

Betulinic acid isolated from Bacopa monniera (L.) Wettst suppresses lipopolysaccharide stimulated interleukin-6 production through modulation of nuclear factor-κB in peripheral blood mononuclear cells

“…Bacopa monniera commonly as Bacopa monnieri, Herpestis monniera, water hyssop, thyme leafed gratiola or Brahmi is a member of the Scrophulariaceae family. It is widely distributed in marshy areas, and has been reported to possess memory enhancing [8], cognitive [9], antioxidant [10], antifertility [11], hepatoprotective [12], anti-cancer [13], anti-ulcer [14] and anti-inflammatory [15] properties. Bacopa monniera is traditionally and anecdotally used for the management of rheumatic disorders.…”

Inhibition of pro-inflammatory mediators: role of Bacopa monniera (L.) Wettst

“…One such plant that has been used to cure inflammation is Bacopa monniera (Brahmi), a member of the Scrophulariaceae family. Renowned in the Ayurvedic system of medicine, this herb is reported to possess cognitive (Zhou et al 2009), anti-fertility (Singh and Singh 2009), hepatoprotective (Vijayan and Helen 2007), anti-cancer (Rohini and Devi 2008), antioxidant (Chowdhury et al 2002), anti-ulcer (Sairam et al 2001) and anti-inflammatory (Viji and Helen 2008) properties. Bacopa monniera extract counters oxidative stress by scavenging reactive oxygen species, inhibiting lipoxygenase activity and reducing divalent metals (Dhanasekaran et al 2007).…”