<i>Bacteroidota</i> and <i>Lachnospiraceae</i> integration into the gut microbiome at key time points in early life are linked to infant neurodevelopment

Oliphant, Kaitlyn; Ali, Mehneez; D’Souza, Mark; Hughes, Patrick D.; Sulakhe, Dinanath; Wang, Annie Z.; Xie, Bingqing; Yeasin, Rummanu; Msall, Michael E.; Andrews, Bree; Claud, Erika C.

doi:10.1080/19490976.2021.1997560

Cited by 56 publications

(52 citation statements)

References 95 publications

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“…The proportion of Bacteroidetes increased significantly after the conversion. Bacteroides is a very diverse phylum of bacteria that is believed to exist in all parts of the mammalian gastrointestinal tract despite differences in pH, nutrients, and oxygen availability [ 53 ]. Additionally, there was a significant decrease in the percentage of Firmicutes after vermicomposting.…”

Section: Resultsmentioning

confidence: 99%

Response of Antibiotic Resistance Genes and Related Microorganisms to Arsenic during Vermicomposting of Cow Dung

Chen

Zhang

et al. 2022

IJERPH

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Antibiotic resistance pollution in livestock manure is a persistent issue that has drawn public attention. Vermicomposting is an ecofriendly biological process that can render livestock manure harmless and resourceful. However, little is known about the impact of vermicomposting on antibiotic resistance in livestock manure under stress caused by potentially toxic arsenic levels. Herein, lab-scale vermicomposting was performed to comprehensively evaluate the shift in antibiotic resistance genes (ARGs) and related microorganisms in fresh earthworm casts as well as vermicompost product health (i.e., nutrient availability and enzyme activity) when they were fed on arsenic-contaminated cow manure. The results showed that the earthworms’ interaction with cow dung led to a significant reduction in ARG concentrations, especially for tetracycline ARGs (tet-ARGs), β-lactam ARGs (bla-ARGs), and quinolone ARGs (qnr-ARGs). However, arsenic significantly enhanced ARG accumulation in earthworm casts in a dose-dependent manner. Moreover, vermicomposting increased the percentage of Bacteroidota in the converted products. Furthermore, arsenic exposure at low concentrations promoted the proliferation of Proteobacteria, whereas high concentrations had little effect on Proteobacteria. Our study provides valuable insight into the changes in the antibiotic resistome and related microorganisms during vermicomposting of arsenic-amended cow manure, and it is crucial to explain the environmental impact of earthworms and improve our understanding of the reciprocal benefits of soil invertebrates.

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Section: Resultsmentioning

confidence: 99%

Response of Antibiotic Resistance Genes and Related Microorganisms to Arsenic during Vermicomposting of Cow Dung

Chen

Zhang

et al. 2022

IJERPH

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“…Unfortunately, as for mechanisms underlying this association, studies that have identified a direct correlation among juvenile myoclonic epilepsy and class Betaproteobacteria and order Burkholderial are lacking. From the MGB axis perspective, the gut microbiota is associated with neurodevelopment, and a recent observational study has reported that gut microbiota such as Bifidobacterium, Bacteroidetes, and Lachnospiraceae can influence neurodevelopment in infants ( Oliphant et al, 2021 ; Beghetti et al, 2022 ). The neurodevelopmental modulation capability of the gut microbiota may be correlated with the release of neuroactive substances, short-chain fatty acids (SCFAs), and alterations in intestinal or blood-brain barrier integrity alteration ( Louis et al, 2010 ; Hsiao et al, 2013 ; Sarkar et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Genetically proxied gut microbiota, gut metabolites with risk of epilepsy and the subtypes: A bi-directional Mendelian randomization study

Ouyang

Chen

Wang

et al. 2022

Front. Mol. Neurosci.

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BackgroundAn increasing number of observational studies have revealed an association among the gut microbiota, gut metabolites, and epilepsy. However, this association is easily influenced by confounders such as diet, and the causality of this association remains obscure.MethodsAiming to explore the causal relationship and ascertain specific gut microbe taxa for epilepsy, we conducted a bi-directional Mendelian randomization (MR) study based on the genome-wide association study (GWAS) data of epilepsy from the International League Against Epilepsy, with the gut microbiota GWAS results from MiBioGen, and summary-level GWAS data of gut microbiota-dependent metabolites trimethylamine N-oxide and its predecessors.ResultsNine phyla, 15 classes, 19 orders, 30 families, and 96 genera were analyzed. A suggestive association of host-genetic-driven increase in family Veillonellaceae with a higher risk of childhood absence epilepsy (odds ratio [OR]: 1.033, confidential interval [CI]: 1.015–1.051, PIVW = 0.0003), class Melainabacteria with a lower risk of generalized epilepsy with tonic-clonic seizures (OR = 0.986, CI = 0.979–0.994, PIVW = 0.0002), class Betaproteobacteria (OR = 0.958, CI = 0.937–0.979, PIVW = 0.0001), and order Burkholderiales (OR = 0.960, CI = 0.937–0.984, PIVW = 0.0010) with a lower risk of juvenile myoclonic epilepsy were identified after multiple-testing correction. Our sensitivity analysis revealed no evidence of pleiotropy, reverse causality, weak instrument bias, or heterogeneity.ConclusionThis is the first MR analysis to explore the potential causal relationship among the gut microbiota, metabolites, and epilepsy. Four gut microbiota features (two class levels, one order level, and one family level) were identified as potential interventional targets for patients with childhood absence epilepsy, generalized epilepsy with tonic-clonic seizures, and juvenile myoclonic epilepsy. Previous associations in numerous observational studies may had been interfered by confounders. More rigorous studies were needed to ascertain the relationship among the gut microbiota, metabolites, and epilepsy.

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“…Supplementation with QE increased the relative abundance of Lachnospiraceae, which is involved in butyrate production, and has the capability to form conjugated linoleic acid from linoleic acid ( Neyrinck et al, 2012 ). In addition, Lachnospiraceae was reported to play important roles in dietary glycan foraging, beneficial metabolite production, and immunity, as well as promoting neurodevelopment in animals ( Oliphant et al, 2021 ). Moreover, Rikenellaceae, which is enhanced by vitamin E supplementation in this study, is responsible for producing organic acids, such as propionic and succinic acids by the fermentation of glucose, melibiose, mannose, and lactose, to form iso-methyl branched-chain fatty acids or long-chain saturated acids ( Graf, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Synergy of Dietary Quercetin and Vitamin E Improves Cecal Microbiota and Its Metabolite Profile in Aged Breeder Hens

Amevor

Cui

et al. 2022

Front. Microbiol.

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In the present study, the synergistic effects of quercetin (Q) and vitamin E (E) on cecal microbiota composition and function, as well as the microbial metabolic profile in aged breeder hens were investigated. A total of 400 (65 weeks old) Tianfu breeder hens were randomly allotted to four experimental groups (four replicates per group). The birds were fed diets containing quercetin at 0.4 g/kg, vitamin E (0.2 g/kg), quercetin and vitamin E (QE; 0.4 g/kg and 0.2 g/kg), and a basal diet for a period of 10 wks. After the 10 week experimental period, the cecal contents of 8 aged breeder hens per group were sampled aseptically and subjected to high-throughput 16S rRNA gene sequencing and untargeted metabolomic analysis. The results showed that the relative abundances of phyla Bacteroidota, Firmicutes, and Actinobacteriota were the most prominent among all the dietary groups. Compared to the control group, the relative abundance of the families Bifidobacteriaceae, Lachnospiraceae, Tannerellaceae, Mathonobacteriaceae, Barnesiellaceae, and Prevotellaceae were enriched in the QE group; and Bacteroidaceae, Desulfovibrionaceae, Peptotostretococcaceae, and Fusobacteriaceae were enriched in the Q group, whereas those of Lactobacillaceae, Veillonellaceae, Ruminococcaceae, Akkermansiaceae, and Rikenellaceae were enriched in the E group compared to the control group. Untargeted metabolomics analyses revealed that Q, E, and QE modified the abundance of several metabolites in prominent pathways including ubiquinone and other terpenoid–quinone biosynthesis, regulation of actin cytoskeleton, insulin secretion, pancreatic secretion, nicotine addiction, and metabolism of xenobiotics by cytochrome P450. Furthermore, key cecal microbiota, significantly correlated with important metabolites, for example, (S)-equol positively correlated with Alistipes and Chlamydia in E_vs_C, and negatively correlated with Olsenella, Paraprevotella, and Mucispirillum but, a contrary trend was observed with Parabacteroides in QE_vs_C. This study establishes that the synergy of quercetin and vitamin E alters the cecal microbial composition and metabolite profile in aged breeder hens, which lays a foundation for chicken improvement programs.

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Bacteroidota and Lachnospiraceae integration into the gut microbiome at key time points in early life are linked to infant neurodevelopment

Cited by 56 publications

References 95 publications

Response of Antibiotic Resistance Genes and Related Microorganisms to Arsenic during Vermicomposting of Cow Dung

Response of Antibiotic Resistance Genes and Related Microorganisms to Arsenic during Vermicomposting of Cow Dung

Genetically proxied gut microbiota, gut metabolites with risk of epilepsy and the subtypes: A bi-directional Mendelian randomization study

Synergy of Dietary Quercetin and Vitamin E Improves Cecal Microbiota and Its Metabolite Profile in Aged Breeder Hens

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