BCR – ABL1 expression in multiple myeloma cells: A case of mistaken identity?

Reply to MacLeod et al.: Multiple myeloma plasma cells have chameleon characteristics

“…It appears that MacLeod et al performed only cytogenetic tests (i.e., FISH and RT-PCR) to make a determination of contamination, and we agree that our cells possessed the e14a2 form of BCR-ABL fusion, as we reported using similar genetic tests (1,2). Together with e13a2 fusion form, e14a2 and e13a2 represent the most common forms of BCR-ABL.…”

“…MacLeod et al question whether our lot of H929 multiple myeloma (MM) plasma cells were contaminated with K562 chronic myeloid leukemia (CML) cells (1). Before addressing this issue, we want to state that the central purpose of our paper was to demonstrate that hypothesis-directed proteomics using immunoprecipitation (IP)-liquid chromatography-tandem mass spectrometry (LC-MS/MS) was capable of detecting a cancer cell's defective and activated signaling pathways.…”

High-Throughput Molecular Cancer Cell Line Characterization Using Digital Multiplex Ligation-Dependent Probe Amplification for Improved Standardization of in Vitro Research

“…Beyond the technical limitations of short tandem repeat typing, most currently available genome-wide profiling tools require significant financial or computational resources, currently limiting their applicability for longitudinal, high-throughput quality control. 8 DigitalMLPA is an analysis tool that requires low DNA input (<20 ng) and limited hands-on time for widely scalable library preparation for common nextgeneration sequencers (Illumina platforms). Results can be generated on standard desktop computers without specific bioinformatics skills.…”

Harnessing a catalytic lysine residue for the one-step preparation of homogeneous antibody-drug conjugates

“…(Note that cell line NCI-H929 is listed in Version 8.0 of ICLAC’s Database of Cross-Contaminated or Misidentified Cell Lines ( http://iclac.org/databases/cross-contaminations ). However, its suspected misidentification 63 as a MM cell line by contamination with chronic myeloid leukemia (CML) cell line K562 was challenged 64 . We show in Supplementary Figs.…”