Bifidobacterium breve CCFM1078 Alleviates Collagen-Induced Arthritis in Rats via Modulating the Gut Microbiota and Repairing the Intestinal Barrier Damage

Abstract

This study focused on the effects of Bifidobacterium breve CCFM1078 on the intestinal barrier and systemic inflammation of collagen-induced arthritis (CIA) rats. Female rats were divided into three groups with daily intragastric administration of either saline (control group and model group) or B. breve CCFM1078 (CCFM1078 group, 3 × 10 9 cfu/rat per day) for 5 weeks. In the Model and CCFM1078 groups, arthritis was induced by subcutaneous collagen injection. We found that B. breve CCFM1078 can repair the intestinal barrier, reduce LPS translocation, regulate gut microbiota composition, and increase short-chain fatty acids in the intestine. Then, it can reduce pro-inflammatory cytokines release, adjust immune dysfunction, and inhibit TLR4-MyD88-dependent pathways and downstream inflammatory pathways to alleviate joint inflammation in CIA rats. These findings suggest that B. breve CCFM1078 may alleviate joint inflammation by adjusting the profile of gut microbiota and enhancing the intestinal barrier.

Bifidobacterium longum subsp. infantis B6MNI Alleviates Collagen-Induced Arthritis in Rats via Regulating 5-HIAA and Pim-1/JAK/STAT3 Inflammation Pathways

“…The second immunization was performed the following week. All immunization injection methods and dosages were consistent with the previous study . The guidelines and standards for animal welfare issued by the Jiangnan University Experimental Animal Center were followed to maintain the temperature and humidity levels in the housing spaces of the animals.…”

“…The joint pathological sections were prepared as described in the previous study . In brief, excess skin and muscle tissue were removed from the knee and ankle joints after fixation with paraformaldehyde.…”

“…All immunization injection methods and dosages were consistent with the previous study. 13 The guidelines and standards for animal welfare issued by the Jiangnan University Experimental Animal Center were followed to maintain the temperature and humidity levels in the housing spaces of the animals. All animal trial protocols were reviewed and approved by the Jiangnan University Experimental Animal Management and Animal Welfare Ethics Committee.…”

“…12 The administration of Bifidobacterium breve CCFM1078 was shown to repair the intestinal barrier of CIA rats, reduce LPS translocation from the intestine, and alleviate inflammation in the spleen via the TLR4-MyD88 pathway. 13 In RA patients, the administration of the probiotic mixture Lactobacillus acidophilus La-14, Lactobacillus casei Lc-11, Lactococcus lactis Ll-23, Bifidobacterium lactis Bl-04, and Bifidobacterium bifidum Bb-06 significantly reduced the white blood cell count, TNF-α, and IL-6 levels in the plasma. 14 This can alleviate symptoms and improve the quality of life for patients suffering from RA.…”

“…Administration of Lactobacillus plantarum HG20 in collagen-induced arthritis (CIA) rats significantly reduced joint swelling and downregulated inflammatory and apoptotic pathways of splenic cells . The administration of Bifidobacterium breve CCFM1078 was shown to repair the intestinal barrier of CIA rats, reduce LPS translocation from the intestine, and alleviate inflammation in the spleen via the TLR4-MyD88 pathway . In RA patients, the administration of the probiotic mixture Lactobacillus acidophilus La-14, Lactobacillus casei Lc-11, Lactococcus lactis Ll-23, Bifidobacterium lactis Bl-04, and Bifidobacterium bifidum Bb-06 significantly reduced the white blood cell count, TNF-α, and IL-6 levels in the plasma .…”