BifidobacteriumCECT 7765 improves metabolic and immunological alterations associated with obesity in high-fat diet-fed mice

Abstract

Objectives: To evaluate the effects of administration of Bifidobacterium pseudocatenulatum CECT 7765 on metabolic and immune alterations in obese mice. Design and Methods: Adult male wild-type C57BL-6 mice were fed a standard diet or high-fat diet (HFD), supplemented or not with B. pseudocatenulatum CECT 7765 for 7 weeks. The assessments included biochemical and immunological parameters, insulin resistance, glucose tolerance, histology of liver, white-adipose and intestinal tissues, immunocompetent cell functions, and microbiota-related features. Results: B. pseudocatenulatum CECT 7765 reduced serum cholesterol, triglyceride, and glucose levels and decreased insulin resistance and improved glucose tolerance in obese mice. This strain reduced serum levels of leptin, interleukin (IL)-6 and monocyte chemotactic protein-1, while increased those of IL-4 in HFD-fed mice. B. pseudocatenulatum CECT7765 reduced liver steatosis and the number of larger adipocytes and number of fat micelles in enterocytes of obese mice. The strain also improved the function of macrophages and dendritic cells in relation to phagocytosis, cytokine production, and induction of T-lymphocyte proliferation. The strain administration increased bifidobacteria and reduced enterobacteria and the inflammatory properties of the gut content in HFD-fed mice. Conclusion: B. pseudocatenulatum CECT 7765 was shown to ameliorate both metabolic and immunological dysfunctions related to obesity in HFD-fed mice.

Gut microbiota and metabolic syndrome

“…The experimental studies [98][99][100][101][102][103][104] demonstrating the beneficial effects observed in HFD-induced metabolic syndrome, after the administration of probiotics containing Bifidobacterium strains, are reported in Table 1.…”

Yellow pea fiber improves glycemia and reduces Clostridium leptum in diet-induced obese rats

“…Although not unanimously shown [10][11][12], on the one hand, obesity appears to be characterized by a decrease in Bacteroidetes and a corresponding increase in Firmicutes, which has been associated with increased energy extraction during digestion [13]. On the other hand, certain strains of bifidobacteria have been linked with improvements in metabolic health, including improved glucose tolerance, reduced serum cholesterol and triglycerides, and lower serum levels of leptin and interleukin 6 [14,15]. Although prebiotics are known to increase bifidobacteria and reduce Firmicutes [16,17], examination of the effects of other nondigestible carbohydrates, such as resistant starches and their fermentation end products (ie, short chain fatty acids [SCFAs]), are increasingly being examined for their effects on gut microbiota [18].…”

Nonalcoholic Fatty Liver Disease, the Gut Microbiome, and Diet

“…and decreased Firmicutes (53). Cano et al (59) reported that Bifidobacterium pseudocatenulatum consumption improved glucose tolerance and inflammatory status while decreasing serum LPS and hepatic steatosis in HFD-fed mice. Another study showed that prebiotic oligofructose in combination with probiotic Bifidobacterium animalis subsp.…”