Brassicaand Its Close Allies: Cytogenetics and Evolution

Prakash, Shyam; Bhat, S. R.; Quirós, Carlos F.; Kirti, P. B.; Chopra, V. L.

doi:10.1002/9780470593783.ch2

Cited by 86 publications

(112 citation statements)

References 569 publications

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“…GISH analysis identified 19 chromosome pairs, 10 from the AA genome of B. rapa and 9 from the RR genome of R. sativus (Figure 2). These results indicated that the BB#5 of baemoochae undergoes diploid-like division during meiosis [2].…”

Section: Cytologymentioning

confidence: 55%

“…Inter-generic allopolyploids have not evolved into stable crops despite numerous attempts [2]. Chen and Wu (2008) [7] reported stable progeny of ×Brassicora-phanus, an allopolyploid between R. sativus and B. oleracea, which were derived from mass selection for 10 generations, although they were not developed into cultivated crops.…”

Section: Discussionmentioning

confidence: 99%

“…Following Karpechenko's report in 1927 [1], numerous studies have focused on the allopolyploid, inter-generic hybrid between Brassica and Raphanus, desig-nated ×Brassicoraphanus [2] [3]. Most ×Brassicoraphanus hybrids were unstable; few have been used as breeding material [4] [5] [6].…”

Section: Introductionmentioning

confidence: 99%

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Developing Stable Cultivar through Microspore Mutagenesis in ×Brassicoraphanus koranhort, Inter-Generic Allopolyploid between Brassica rapa and Raphanus sativus

Lee¹,

Hwang²,

Kim³

et al. 2017

AJPS

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A stable progeny was developed through induced mutation, using microspore culture, of the hybrid (F 1 F 1 ) produced by crossing a newly synthesized, unstable allopolyploid (F 1 ) and a stable cultivar, BB#1(F 1 ) in xBrassicoraphanus. An F 1 F 1 plant was subjected to the induced mutation system established during production of BB#1. Morphological characteristics of the progeny such as color, and leaf number and length, differed from those of BB#1. The bolting time of the progeny in spring cropping was very late compared to BB#1, allowing it to be grown to an adult plant in spring. Genomic in situ hybridization analysis of pollen mother cells at prophase I identified 19 bivalents, 10 from Brassica rapa and 9 from Raphanus sativus. The glucoraphenin content was almost identical to that of BB#1. Two cultivars are available in the baemoochae crop now. These results indicate that induced mutation using microspore culture is a viable method of stabilizing inter-generic allopolyploids between B. rapa and R. sativus.

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Section: Cytologymentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

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Developing Stable Cultivar through Microspore Mutagenesis in ×Brassicoraphanus koranhort, Inter-Generic Allopolyploid between Brassica rapa and Raphanus sativus

Lee¹,

Hwang²,

Kim³

et al. 2017

AJPS

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“…Poor fertility is always an obstacle that must be overcome in allopolyploid breeding (Prakash et al, 2009). However, the intergeneric hybrid line BB#5 displays exceptionally high seed fertility and stable inheritance of morphological characters as demonstrated in another xBrassicoraphanus line (Lee et al, 2011).…”

Section: Meiosis Pairing Analysismentioning

confidence: 99%

“…Owing to their usefulness, many studies have been carried out to investigate breeding of these species (McNaughton, 1979;Namai et al, 1980;Olsson and Ellerstrom, 1980;Prakash et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

FISH Karyotype and GISH Meiotic Pairing Analyses of a Stable Intergeneric Hybrid xBrassicoraphanus Line BB#5

Belandres¹,

Waminal²,

Hwang³

et al. 2015

HST

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xBrassicoraphanus line BB#5, a new synthetic intergeneric hybrid between Brassica rapa L. ssp. pekinensis and Raphanus sativus L. var. rafiphera induced by N-methyl-N-nitroso-urethane mutagenesis in microspore culture, shows high seed fertility and morphological uniformity. Dual-color fluorescence in situ hybridization (FISH) using 5S and 45S rDNA probes and genomic in situ hybridization (GISH) using B. rapa genomic DNA probe were carried out to analyze the chromosome composition and the meiosis pairing pattern compared to its parental lines. The somatic chromosome complement is 2n = 38, which consists of 17 metacentric and two submetacentric chromosomes with lengths of 2.18 to 5.01 µm. FISH karyotype analysis showed five and eight pairs of 5S and 45S rDNA loci. GISH meiosis pairing analysis showed that 19 complete bivalents were most frequent and accounted for 42% of the 100 pollen mother cells examined. Based on chromosome number, size, morphology, rDNA distribution, and meiosis pairing pattern, both parental genomes of B. rapa and R. sativus appear to exist in xBrassicoraphanus line BB#5, demonstrating its genome integrity. Such stable chromosome constitutions and meiotic pairing patterns in somatic and meiotic cells are very rare in natural and synthetic intergeneric hybrids. Chromosomal studies and genetic and phenotypic changes in allopolyploids are discussed. The results presented herein will be useful for further genomic study of xBrassicoraphanus lines and their improvement as promising new breeding varieties.

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Plant Interspecific Hybridization: Outcomes and Issues at the Intersection of Species

Morgan

Timmerman-Vaughan

Conner

et al. 2010

Plant Breeding Reviews

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Brassicaand Its Close Allies: Cytogenetics and Evolution

Cited by 86 publications

References 569 publications

Developing Stable Cultivar through Microspore Mutagenesis in ×<i>Brassicoraphanus koranhort</i>, Inter-Generic Allopolyploid between <i>Brassica rapa</i> and <i>Raphanus sativus</i>

Developing Stable Cultivar through Microspore Mutagenesis in ×<i>Brassicoraphanus koranhort</i>, Inter-Generic Allopolyploid between <i>Brassica rapa</i> and <i>Raphanus sativus</i>

FISH Karyotype and GISH Meiotic Pairing Analyses of a Stable Intergeneric Hybrid xBrassicoraphanus Line BB#5

Plant Interspecific Hybridization: Outcomes and Issues at the Intersection of Species

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Brassicaand Its Close Allies: Cytogenetics and Evolution

Cited by 86 publications

References 569 publications

Developing Stable Cultivar through Microspore Mutagenesis in &#215;&lt;i&gt;Brassicoraphanus koranhort&lt;/i&gt;, Inter-Generic Allopolyploid between &lt;i&gt;Brassica rapa&lt;/i&gt; and &lt;i&gt;Raphanus sativus&lt;/i&gt;

Developing Stable Cultivar through Microspore Mutagenesis in &#215;&lt;i&gt;Brassicoraphanus koranhort&lt;/i&gt;, Inter-Generic Allopolyploid between &lt;i&gt;Brassica rapa&lt;/i&gt; and &lt;i&gt;Raphanus sativus&lt;/i&gt;

FISH Karyotype and GISH Meiotic Pairing Analyses of a Stable Intergeneric Hybrid xBrassicoraphanus Line BB#5

Plant Interspecific Hybridization: Outcomes and Issues at the Intersection of Species

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Developing Stable Cultivar through Microspore Mutagenesis in ×<i>Brassicoraphanus koranhort</i>, Inter-Generic Allopolyploid between <i>Brassica rapa</i> and <i>Raphanus sativus</i>

Developing Stable Cultivar through Microspore Mutagenesis in ×<i>Brassicoraphanus koranhort</i>, Inter-Generic Allopolyploid between <i>Brassica rapa</i> and <i>Raphanus sativus</i>