<i>Brucella</i>
            -Agglutinating Antibodies: Relation of Mercaptoethanol Stability to Complement Fixation

Anderson, Richard W.; Jenness, Robert; Brumfield, Helene P.; Gough, Patricia M.

doi:10.1126/science.143.3612.1334

Cited by 48 publications

(13 citation statements)

References 15 publications

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“…The former workers showed that three purified immunoglobulins, known as IgG, IgA, andIgM, obtained from the serum of cases of brucellosis contained brucella agglutinating antibodies, and Wilkinson, using mainly cattle sera, found that when each of these immunoglobulins was removed by specific precipitating rabbit anti-human IgG, IgA, and IgM sera this resulted in .he inhibition of their serological activity. Anderson et al '1964) and Reddin et al (1965) reported changes in serological activity during the course of the disease, and it seemed likely that analysis of a serum at different stages of the disease to letermine in which fraction its serological activity lies might give information of value in diagnosis, as has been indicated in a previous study (Kerr et al, 1966).…”

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confidence: 86%

Antibodies in human brucellosis.

Coghlan¹,

Weir²

1967

BMJ

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confidence: 86%

Antibodies in human brucellosis.

Coghlan¹,

Weir²

1967

BMJ

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“…Detection of specific IgM antibodies in ELISA is useful in diagnosing patients with acute illness, given the fact that the IgM antibody response tends to develop somewhat earlier than the IgG response. IgM detection also is used to discriminate between acute and chronic diseases, and for this SAT has been used as well (2,16,22). A significant reduction in titer in this assay when performed in the presence of reducing agent indicates the presence of specific IgM antibodies.…”

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confidence: 99%

ImmunochromatographicBrucella-Specific Immunoglobulin M and G Lateral Flow Assays for Rapid Serodiagnosis of Human Brucellosis

Smits

Abdoel

Solera

et al. 2003

Clin Vaccine Immunol

101

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To fulfill the need for a simple and rapid diagnostic test for human brucellosis, we used the immunochromatographic lateral flow assay format to develop two assays, one for the detection of Brucella-specific immunoglobulin M (IgM) antibodies and one for the detection of Brucella-specific IgG antibodies. The diagnostic values of these tests were examined. The tests are shown to detect acute, persistent, and relapsing disease and can be used to monitor treatment. The sensitivity of Brucella IgM and IgG flow assays calculated for the combined assay results is 96%, and specificity amounts to 99%. The flow assay requires neither specialized training nor equipment, the assay is very easy to perform and to read, and the components are stable without a requirement for refrigeration and well standardized. Together these characteristics indicate that the Brucella IgM and IgG flow assays are ideal for use in clinical settings in rural and suburban areas in which brucellosis is endemic.

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“…abortus infection. However, the agglutinin levels decline in the chronic phase of the disease, probably because of shift in the pattern of immunoglobulin response (Anderson et al, 1964 ;Rice, Tailyour and Cochrane, 1966). Nevertheless, precipitins persist for very long periods, possibly for the duration of infection (Corbel, to be published), suggesting that differences in immunodiffusion patterns might be used to distinguish between the antibody responses to 45/20 vaccine and active infection.…”

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confidence: 99%

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Evaluation of an Immunodiffusion Test for the Detection of Antibodies to Brucella Abortusin Bovine Serum

Corbel

1973

Journal of Medical Microbiology

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CONSIDERABLE attention has been paid to the selection of diagnostic procedures for differentiation of antibodies resulting from vaccination of cattle with Brucella abortus, strain 19, from those resulting from natural infection (Yuskovets, 1956;Anderson et al., 1964;Rose and Amerault, 1964;Schimmel and Erler, 1967). Relatively less attention has been given to the problems in diagnosis arising from the use of Br. abortus strain-45/20 adjuvant vaccine. This vaccine is weakly agglutinogenic but gives rise to significant titres of antibodies active in the complement fixation (CF) and antiglobulin tests. Although, in most cases, the antibody titres in all of these tests decline to insignificant levels within 1 yr of vaccination, some animals show persistent titres sufficiently high to cause difficulties in the interpretation of diagnostic tests. Usually in these cases the CF test shows inconclusive or weakly positive reactions and the titres in the antiglobulin test are also raised.These problems are accentuated in herds in which strain 19 and strain 45/20 vaccines have been used. In such cases, agglutinins active in the serum agglutination (SA) and Rose Bengal plate (RBP) tests may also persist at significant levels for prolonged periods.Clearly, to resolve this situation, a supplementary test is needed that would enable features characteristic of either the vaccinated or the naturally infected state to be identified. A procedure of potential value in this situation is the immunodiffusion test. This has the advantage of enabling the reaction of specific antigenic components with their respective antibodies to be identified. Thus, antibodies to components specific either to the infected or the vaccinated state could, hypothetically, be identified.In the present study, a comparison of an immunodiffusion test with standard diagnostic tests was made on serum samples from unvaccinated cattle, from cattle vaccinated with strain 19 or strain 45/20, and from cattle known to be infected with virulent Br. abortus strains. MATERIALS AND METHODS Brucella

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Brucella -Agglutinating Antibodies: Relation of Mercaptoethanol Stability to Complement Fixation

Cited by 48 publications

References 15 publications

Antibodies in human brucellosis.

Antibodies in human brucellosis.

ImmunochromatographicBrucella-Specific Immunoglobulin M and G Lateral Flow Assays for Rapid Serodiagnosis of Human Brucellosis

Evaluation of an Immunodiffusion Test for the Detection of Antibodies to Brucella Abortusin Bovine Serum

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