<i>Brucella neotomae</i>Infection in Humans, Costa Rica

Suárez-Esquivel, Marcela; Ruíz-Villalobos, Nazareth; Jiménez-Rojas, César; Barquero-Calvo, Elías; Víquez-Ruiz, Eunice; Rojas‐Campos, Norman; Baker, Kate S.; Oviedo-Sánchez, Gerardo; Amuy, Ernesto; Chaves-Olarte, Esteban; Thomson, Nicholas R.; Moreno, Edgardo; Guzmán-Verri, Caterina

doi:10.3201/eid2306.162018

Cited by 42 publications

(30 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the current knowledge, the central questions of brucellosis, regarding host adaptation and virulence variation require a solution. The fact that Brucella from wood rats, hares, and cold-blooded animals can infect humans [ 78 , 114 , 173 ] depicts the zoonotic potential of all members of the genus and the ability of these bacteria to adapt from the preferred host to other animals and cause disease.…”

Section: Discussionmentioning

confidence: 99%

“…For this reason, many Brucella isolates recovered from various animal species had been misclassified. One relevant example is depicted by the discovery of B. neotomae, a parasite of wood rats, as a zoonotic agent of two cases of human brucellosis [ 78 , 79 ]. Initially, this bacterium was misclassified as B. abortus by classical bacteriological methods.…”

Section: Expanding the Number Of Brucella Specimentioning

confidence: 99%

“…Initially, this bacterium was misclassified as B. abortus by classical bacteriological methods. The correct identification of this species was only possible by detailed phenotypic and genotypic analysis, including WGS [ 78 ]. After these reports, investigators confirmed the infective potential of B. neotomae in mice [ 80 , 81 ].…”

Section: Expanding the Number Of Brucella Specimentioning

confidence: 99%

See 2 more Smart Citations

Brucella Genomics: Macro and Micro Evolution

Suárez-Esquivel

Chaves-Olarte

Moreno

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Brucella organisms are responsible for one of the most widespread bacterial zoonoses, named brucellosis. The disease affects several species of animals, including humans. One of the most intriguing aspects of the brucellae is that the various species show a ~97% similarity at the genome level. Still, the distinct Brucella species display different host preferences, zoonotic risk, and virulence. After 133 years of research, there are many aspects of the Brucella biology that remain poorly understood, such as host adaptation and virulence mechanisms. A strategy to understand these characteristics focuses on the relationship between the genomic diversity and host preference of the various Brucella species. Pseudogenization, genome reduction, single nucleotide polymorphism variation, number of tandem repeats, and mobile genetic elements are unveiled markers for host adaptation and virulence. Understanding the mechanisms of genome variability in the Brucella genus is relevant to comprehend the emergence of pathogens.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Expanding the Number Of Brucella Specimentioning

confidence: 99%

Section: Expanding the Number Of Brucella Specimentioning

confidence: 99%

See 1 more Smart Citation

Brucella Genomics: Macro and Micro Evolution

Suárez-Esquivel

Chaves-Olarte

Moreno

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although not previously thought capable of infecting humans, B. neotomae was recently isolated from the cerebrospinal fluid of two patients from Costa Rica (26). Potential epidemiological associations and patient comorbidities were not investigated.…”

Section: Discussionmentioning

confidence: 99%

Brucella neotomae Recapitulates Attributes of Zoonotic Human Disease in a Murine Infection Model

Kang

Brown

2019

Infect Immun

View full text Add to dashboard Cite

Members of the genus Brucella are Gram-negative pathogens that cause chronic systemic infection in farm animals and zoonotic infection in humans. Study of the genus Brucella has been hindered by the need for biosafety level 3 select agent containment. Brucella neotomae, originally isolated from the desert pack rat, presented an opportunity to develop an alternative, non-select agent experimental model. Our prior in vitro work indicated that the cell biology and type IV secretion system (T4SS) dependence of B. neotomae intracellular replication were similar to observations for humanpathogenic select agent Brucella species. Therefore, here, we investigated the pathobiology of B. neotomae infection in the BALB/c mouse. During a sustained infectious course, B. neotomae replicated and persisted in reticuloendothelial organs. Bioluminescent imaging and histopathological and PCR-based analysis demonstrated that the T4SS contributed to efficient early infection of the liver, spleen, and lymph nodes; granuloma formation and hepatosplenomegaly; and early induction of Th1-associated cytokine gene expression. The infectious course and pathologies in the murine model showed similarity to prior observations of primate and native host infection with zoonotic Brucella species. Therefore, the B. neotomae BALB/c infection model offers a promising system to accelerate and complement experimental work in the genus Brucella. KEYWORDS Brucella, Brucella neotomae, brucellosis, bioluminescent imaging, murine model, pathogenesis, pathology, type IV secretion system, undulant fever M embers of the genus Brucella, Gram-negative coccobacilli, cause systemic bacterial infections in domesticated farm animals and other vertebrates (1). Humans may be infected through ingestion of unpasteurized dairy products, inhalation of organisms from birthing farm animals, and during occupational exposure in abattoir and meat packing industries. Disease in humans is characterized by chronicity and a waxing and waning fever (so-called undulant fever). Lymph node infection and enlargement are common (2); endovascular and osteoarticular infection are unwelcome and not infrequent complications (3,4).Based on the low aerosol infectious dose and debilitating disease, the major zoonotic species, Brucella melitensis, Brucella abortus, and Brucella suis, are classified as biosafety level 3, category B overlap select agents (5). Biosafety containment and select agent regulations represent significant impediments to experimental investigation.Brucella organisms have been studied in several in vitro and in vivo model systems (6)(7)(8)(9). In vitro assays have demonstrated the capability of Brucella species to grow within specialized vacuoles inside of macrophages, endothelial cells, and other cell types. In particular, Brucella species alter the normal process of phagosomal maturation. Remodeling of the phagosome occurs in a temporal fashion in which organisms are first found within endocytic vacuoles that subsequently take on properties of the endoplasmic reticulum an...

show abstract

“…Brucellosis became a notifiable disease in CR in 1915, after the first isolation of "Bang´s bacillus" from the blood of a human patient [15,16]. More recently, and thanks to improved surveillance and diagnosis of the disease by public health authorities [8], an increasing number of human cases have been reported, with positive hemocultures and description of the first human cases caused by Brucella neotomae [17,18].…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Persistence of Brucella abortus lineages revealed by genomic characterization and phylodynamic analysis

et al. 2020

Self Cite

View full text Add to dashboard Cite

Brucellosis, caused by Brucella abortus, is a major disease of cattle and humans worldwide distributed. Eradication and control of the disease has been difficult in Central and South America, Central Asia, the Mediterranean and the Middle East. Epidemiological strategies combined with phylogenetic methods provide the high-resolution power needed to study relationships between surveillance data and pathogen population dynamics, using genetic diversity and spatiotemporal distributions. This information is crucial for prevention and control of disease spreading at a local and worldwide level. In Costa Rica (CR), the disease was first reported at the beginning of the 20 th century and has not been controlled despite many efforts. We characterized 188 B. abortus isolates from CR recovered from cattle, humans and water buffalo, from 2003 to 2018, and whole genome sequencing (WGS) was performed in 95 of them. They were also assessed based on geographic origin, date of introduction, and phylogenetic associations in a worldwide and national context. Our results show circulation of five B. abortus lineages (I to V) in CR, phylogenetically related to isolates from the United States, United Kingdom, and South America. Lineage I was dominant and probably introduced at the end of the 19 th century. Lineage II, represented by a single isolate from a water buffalo, clustered with a Colombian sample, and was likely introduced after 1845. Lineages III and IV were likely introduced during the early 2000s. Fourteen isolates from humans were found within the same lineage (lineage I) regardless of their geographic origin within the country. The main CR lineages, introduced more than 100 years ago, are widely spread throughout the country, in contrast to new introductions that seemed to be more geographically restricted. Following the brucellosis prevalence and the farming practices of several middle-and low-income countries, similar scenarios could be found in other regions worldwide.

show abstract

Brucella neotomaeInfection in Humans, Costa Rica

Cited by 42 publications

References 12 publications

Brucella Genomics: Macro and Micro Evolution

Brucella Genomics: Macro and Micro Evolution

Brucella neotomae Recapitulates Attributes of Zoonotic Human Disease in a Murine Infection Model

Persistence of Brucella abortus lineages revealed by genomic characterization and phylodynamic analysis

Contact Info

Product

Resources

About